Y Lin1, C-S Zhang, S-J Li, Z Li, F-B Sun. 1. Department of General Surgery, the Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China. sunfubodoc@outlook.com.
Abstract
OBJECTIVE: To explore whether long noncoding RNA (lncRNA) LOC554202 could regulate proliferative and migratory abilities of gastric cancer (GC) cells. MATERIALS AND METHODS: Expression level of LOC554202 in GC cell lines HGC-27 and MGC-803, as well as normal gastric mucosal cell line GES-1 was detected by quantitative real-time polymerase chain reaction (qRT-PCR). LOC554202 knockdown or overexpression in HGC-27 and MGC-803 cells was achieved by transfection of LOC554202-siRNA or pcDNA-LOC554202, respectively. Cell cycle in GC cells was accessed by flow cytometry. Migratory ability of GC cells was determined by wound healing assay and transwell assay. Finally, protein expressions of p21 and E-cadherin in GC cells were detected by Western blot. RESULTS: LOC554202 expression was higher in GC cells than that of gastric mucosal cells (p<0.01). Overexpression of LOC554202 in MGC-803 cells enhanced proliferative and migratory abilities, but decreased protein expressions of p21 and E-cadherin (p<0.01). On the contrary, LOC554202 overexpression in HGC-27 cells decreased proliferative and migratory abilities, but increased protein expressions of p21 and E-cadherin (p<0.01). CONCLUSIONS: LncRNA LOC554202 is highly expressed in GC cells. It could promote proliferative and migratory abilities by downregulating expression levels of p21 and E-cadherin in GC cells.
OBJECTIVE: To explore whether long noncoding RNA (lncRNA) LOC554202 could regulate proliferative and migratory abilities of gastric cancer (GC) cells. MATERIALS AND METHODS: Expression level of LOC554202 in GC cell lines HGC-27 and MGC-803, as well as normal gastric mucosal cell line GES-1 was detected by quantitative real-time polymerase chain reaction (qRT-PCR). LOC554202 knockdown or overexpression in HGC-27 and MGC-803 cells was achieved by transfection of LOC554202-siRNA or pcDNA-LOC554202, respectively. Cell cycle in GC cells was accessed by flow cytometry. Migratory ability of GC cells was determined by wound healing assay and transwell assay. Finally, protein expressions of p21 and E-cadherin in GC cells were detected by Western blot. RESULTS: LOC554202 expression was higher in GC cells than that of gastric mucosal cells (p<0.01). Overexpression of LOC554202 in MGC-803 cells enhanced proliferative and migratory abilities, but decreased protein expressions of p21 and E-cadherin (p<0.01). On the contrary, LOC554202 overexpression in HGC-27 cells decreased proliferative and migratory abilities, but increased protein expressions of p21 and E-cadherin (p<0.01). CONCLUSIONS: LncRNA LOC554202 is highly expressed in GC cells. It could promote proliferative and migratory abilities by downregulating expression levels of p21 and E-cadherin in GC cells.