Literature DB >> 3056929

EnvZ, a transmembrane environmental sensor of Escherichia coli K-12, is phosphorylated in vitro.

M M Igo1, T J Silhavy.   

Abstract

By fusing the transcriptional and translational start signals of lacZ to envZ, we have obtained high-level synthesis of a truncated EnvZ protein (EnvZ115) in which the first 38 amino acids of EnvZ are replaced with the first 8 amino acids of LacZ. Using this construct, we have partially purified the EnvZ115 protein and demonstrated that this protein can be phosphorylated in vitro. We suggest that phosphorylation may be an important feature of EnvZ function.

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Year:  1988        PMID： 3056929      PMCID： PMC211718          DOI： 10.1128/jb.170.12.5971-5973.1988

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  18 in total

1.  Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands.

Authors:  B Lugtenberg; J Meijers; R Peters; P van der Hoek; L van Alphen
Journal:  FEBS Lett       Date:  1975-10-15       Impact factor: 4.124

2.  Homologies between the Salmonella typhimurium CheY protein and proteins involved in the regulation of chemotaxis, membrane protein synthesis, and sporulation.

Authors:  A Stock; D E Koshland; J Stock
Journal:  Proc Natl Acad Sci U S A       Date:  1985-12       Impact factor: 11.205

3.  Influence of osmolarity of the growth medium on the outer membrane protein pattern of Escherichia coli.

Authors:  W V Alphen; B Lugtenberg
Journal:  J Bacteriol       Date:  1977-08       Impact factor: 3.490

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Purification and characterization of the OmpR protein, a positive regulator involved in osmoregulatory expression of the ompF and ompC genes in Escherichia coli.

Authors:  Y L Jo; F Nara; S Ichihara; T Mizuno; S Mizushima
Journal:  J Biol Chem       Date:  1986-11-15       Impact factor: 5.157

6.  Kinetic analysis of lamB mutants suggests the signal sequence plays multiple roles in protein export.

Authors:  J Stader; S A Benson; T J Silhavy
Journal:  J Biol Chem       Date:  1986-11-15       Impact factor: 5.157

7.  Osmoregulation of gene expression. II. DNA sequence of the envZ gene of the ompB operon of Escherichia coli and characterization of its gene product.

Authors:  T Mizuno; E T Wurtzel; M Inouye
Journal:  J Biol Chem       Date:  1982-11-25       Impact factor: 5.157

8.  Genetic analysis of the ompB locus in Escherichia coli K-12.

Authors:  M N Hall; T J Silhavy
Journal:  J Mol Biol       Date:  1981-09-05       Impact factor: 5.469

9.  Influence of molecular size and osmolarity of sugars and dextrans on the synthesis of outer membrane proteins O-8 and O-9 of Escherichia coli K-12.

Authors:  H Kawaji; T Mizuno; S Mizushima
Journal:  J Bacteriol       Date:  1979-12       Impact factor: 3.490

10.  Covalent modification of the glnG product, NRI, by the glnL product, NRII, regulates the transcription of the glnALG operon in Escherichia coli.

Authors:  A J Ninfa; B Magasanik
Journal:  Proc Natl Acad Sci U S A       Date:  1986-08       Impact factor: 11.205
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  62 in total

1.  C-terminal DNA binding stimulates N-terminal phosphorylation of the outer membrane protein regulator OmpR from Escherichia coli.

Authors:  S K Ames; N Frankema; L J Kenney
Journal:  Proc Natl Acad Sci U S A       Date:  1999-10-12       Impact factor: 11.205

2.  The histidine kinase domain of UhpB inhibits UhpA action at the Escherichia coli uhpT promoter.

Authors:  J S Wright; I N Olekhnovich; G Touchie; R J Kadner
Journal:  J Bacteriol       Date:  2000-11       Impact factor: 3.490

3.  Transcriptional activation of the Rhodobacter sphaeroides cytochrome c(2) gene P2 promoter by the response regulator PrrA.

Authors:  James C Comolli; Audrey J Carl; Christine Hall; Timothy Donohue
Journal:  J Bacteriol       Date:  2002-01       Impact factor: 3.490

4.  Interdomain linkers of homologous response regulators determine their mechanism of action.

Authors:  Don Walthers; Van K Tran; Linda J Kenney
Journal:  J Bacteriol       Date:  2003-01       Impact factor: 3.490

5.  Genetic and biochemical studies of phosphatase activity of PhoR.

Authors:  Daniel O Carmany; Kristine Hollingsworth; William R McCleary
Journal:  J Bacteriol       Date:  2003-02       Impact factor: 3.490

6.  KdpD and KdpE, proteins that control expression of the kdpABC operon, are members of the two-component sensor-effector class of regulators.

Authors:  M O Walderhaug; J W Polarek; P Voelkner; J M Daniel; J E Hesse; K Altendorf; W Epstein
Journal:  J Bacteriol       Date:  1992-04       Impact factor: 3.490

7.  Cross talk to the phosphate regulon of Escherichia coli by PhoM protein: PhoM is a histidine protein kinase and catalyzes phosphorylation of PhoB and PhoM-open reading frame 2.

Authors:  M Amemura; K Makino; H Shinagawa; A Nakata
Journal:  J Bacteriol       Date:  1990-11       Impact factor: 3.490

8.  The inner membrane histidine kinase EnvZ senses osmolality via helix-coil transitions in the cytoplasm.

Authors:  Loo Chien Wang; Leslie K Morgan; Pahan Godakumbura; Linda J Kenney; Ganesh S Anand
Journal:  EMBO J       Date:  2012-04-27       Impact factor: 11.598

9.  The spoIIJ gene, which regulates early developmental steps in Bacillus subtilis, belongs to a class of environmentally responsive genes.

Authors:  C Antoniewski; B Savelli; P Stragier
Journal:  J Bacteriol       Date:  1990-01       Impact factor: 3.490

Review 10.  Physiological and genetic responses of bacteria to osmotic stress.

Authors:  L N Csonka
Journal:  Microbiol Rev       Date:  1989-03
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