Grégoire B Morand1, Kristian Ikenberg2, Domenic G Vital1, Isabel Cardona3, Holger Moch2, Sandro J Stoeckli4, Gerhard F Huber1. 1. Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Zurich, Zurich, Switzerland. 2. Institute of Pathology and Molecular Pathology, University Hospital Zurich, Zurich, Switzerland. 3. Department of Otolaryngology, Head and Neck Surgery, McGill University, Montreal, Québec, Canada. 4. Department of Otorhinolaryngology, Head and Neck Surgery, Kantonsspital St. Gallen, St. Gallen, Switzerland.
Abstract
BACKGROUND: Epithelial-mesenchymal transition and cancer stem-like cells (CSC) have been linked to increased metastatic potential. We evaluated the prognostic impact of CD44, a CSC biomarker, on depth of invasion (DOI) and outcome in oral squamous cell carcinoma (OSCC). METHODS: Using a multivariable logistic regression model, we evaluated in early OSCCs the relationship between CD44 expression at the invasive tumor front, DOI, sentinel lymph node biopsy, extension of nodal involvement, and survival. We also assessed whether CT and/or MRI could predict DOI preoperatively. RESULTS: CD44 expression was associated with increased DOI (P = .018), worse disease-specific survival (P = .041) but not with positive sentinel lymph node biopsy (P > .05). Each millimeter increase in DOI was associated with a 31.1% higher risk for positive sentinel lymph node biopsy (95% CI: 5.8%-62.4%, P = .013) and with higher metastatic ratio (P = .015). Preoperative estimation of DOI by CT and/or MRI and histopathological DOI showed a strong correlation (P < .0001). CONCLUSIONS: CD44 expression correlates with DOI, which predicts occult lymph node metastasis. Preoperative CT and/or MRI provides an accurate estimation of histopathological DOI. Both pieces of information gained preoperatively can help surgeons tailor their operation in regard to the surgical management of the neck.
BACKGROUND: Epithelial-mesenchymal transition and cancer stem-like cells (CSC) have been linked to increased metastatic potential. We evaluated the prognostic impact of CD44, a CSC biomarker, on depth of invasion (DOI) and outcome in oral squamous cell carcinoma (OSCC). METHODS: Using a multivariable logistic regression model, we evaluated in early OSCCs the relationship between CD44 expression at the invasive tumor front, DOI, sentinel lymph node biopsy, extension of nodal involvement, and survival. We also assessed whether CT and/or MRI could predict DOI preoperatively. RESULTS:CD44 expression was associated with increased DOI (P = .018), worse disease-specific survival (P = .041) but not with positive sentinel lymph node biopsy (P > .05). Each millimeter increase in DOI was associated with a 31.1% higher risk for positive sentinel lymph node biopsy (95% CI: 5.8%-62.4%, P = .013) and with higher metastatic ratio (P = .015). Preoperative estimation of DOI by CT and/or MRI and histopathological DOI showed a strong correlation (P < .0001). CONCLUSIONS:CD44 expression correlates with DOI, which predicts occult lymph node metastasis. Preoperative CT and/or MRI provides an accurate estimation of histopathological DOI. Both pieces of information gained preoperatively can help surgeons tailor their operation in regard to the surgical management of the neck.
Authors: Grégoire B Morand; Martina A Broglie; Paul Schumann; Martin W Huellner; Niels J Rupp Journal: Front Oncol Date: 2020-08-27 Impact factor: 6.244
Authors: Jonas Werner; Martin W Hüllner; Niels J Rupp; Alexander M Huber; Martina A Broglie; Gerhard F Huber; Grégoire B Morand Journal: Sci Rep Date: 2019-06-20 Impact factor: 4.379
Authors: Stephanie A Stalder; Paul Schumann; Martin Lanzer; Martin W Hüllner; Niels J Rupp; Martina A Broglie; Grégoire B Morand Journal: Biology (Basel) Date: 2020-02-02
Authors: Joshua Lubov; Mathilde Labbé; Krystelle Sioufi; Grégoire B Morand; Michael P Hier; Manish Khanna; Khalil Sultanem; Alex M Mlynarek Journal: J Otolaryngol Head Neck Surg Date: 2021-09-07
Authors: Koos Boeve; Mirjam F Mastik; Lorian Slagter-Menkema; Boukje A C van Dijk; Jan L N Roodenburg; Bernard F A M van der Laan; Max J H Witjes; Bert van der Vegt; Ed Schuuring Journal: Head Neck Date: 2021-05-19 Impact factor: 3.147