Hui Gu1, Lizhu Chen1, Jia Xue1, Tianchu Huang1, Xiaowei Wei1, Dan Liu1, Wei Ma1, Songying Cao2, Zhengwei Yuan3. 1. Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital, China Medical University, Shenyang, China. 2. Department of Obstetrics, Gynecology, and Reproductive Sceinces, University of Maryland School of Medicine, Baltimore, MD, USA. 3. Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital, China Medical University, Shenyang, China. Electronic address: yuanzw@hotmail.com.
Abstract
OBJECTIVE: The discovery of cell free fetal microRNAs (miRNAs) in maternal circulation has opened up novel probabilities for non-invasive prenatal diagnosis. This study aims to investigate circulating miRNAs as potential biomarkers in the serum of pregnant women with congenital heart defect (CHD) fetuses. METHOD: A total of 110 pregnant women including 50 CHD cases and 60 healthy controls were included in this study. miRNA microarray followed by real-time PCR was used to explore miRNA expression. Receiver operating characteristic (ROC) curves were calculated to assess the diagnostic capability of miRNAs for fetal CHDs. RESULTS: 38 Serum miRNAs were revealed to be differentially expressed in the CHD group as compared to control group via microarray. Among these, nine down-regulated and three up-regulated miRNAs were validated by real-time PCR. Ten of these miRNAs were rapidly reduced in the normal maternal serum after delivery as compared to before delivery. In particular, we identified a biomarker panel consisting of four miRNAs (miR-142-5p, miR-1275, miR-4666a-3p and miR-3664-3p) capable of distinguishing CHDs from controls (area under the ROC curve (AUC), 0.920; p < 0.0001). CONCLUSION: The discovery of these dysregulated pregnancy-associated miRNAs in maternal serum may be potential biomarkers for non-invasive prenatal diagnosis of fetal CHDs.
OBJECTIVE: The discovery of cell free fetal microRNAs (miRNAs) in maternal circulation has opened up novel probabilities for non-invasive prenatal diagnosis. This study aims to investigate circulating miRNAs as potential biomarkers in the serum of pregnant women with congenital heart defect (CHD) fetuses. METHOD: A total of 110 pregnant women including 50 CHD cases and 60 healthy controls were included in this study. miRNA microarray followed by real-time PCR was used to explore miRNA expression. Receiver operating characteristic (ROC) curves were calculated to assess the diagnostic capability of miRNAs for fetal CHDs. RESULTS: 38 Serum miRNAs were revealed to be differentially expressed in the CHD group as compared to control group via microarray. Among these, nine down-regulated and three up-regulated miRNAs were validated by real-time PCR. Ten of these miRNAs were rapidly reduced in the normal maternal serum after delivery as compared to before delivery. In particular, we identified a biomarker panel consisting of four miRNAs (miR-142-5p, miR-1275, miR-4666a-3p and miR-3664-3p) capable of distinguishing CHDs from controls (area under the ROC curve (AUC), 0.920; p < 0.0001). CONCLUSION: The discovery of these dysregulated pregnancy-associated miRNAs in maternal serum may be potential biomarkers for non-invasive prenatal diagnosis of fetal CHDs.
Authors: Carmen Elena Condrat; Valentin Nicolae Varlas; Florentina Duică; Panagiotis Antoniadis; Cezara Alina Danila; Dragos Cretoiu; Nicolae Suciu; Sanda Maria Crețoiu; Silviu Cristian Voinea Journal: Int J Mol Sci Date: 2021-04-09 Impact factor: 5.923
Authors: Fulya Ekiz Kanik; Iris Celebi; Derin Sevenler; Kahraman Tanriverdi; Nese Lortlar Ünlü; Jane E Freedman; M Selim Ünlü Journal: Sci Rep Date: 2022-09-28 Impact factor: 4.996