Marta Martínez-Pérez1, Ana Conde2, María-Angeles Arenas3, Ignacio Mahíllo-Fernandez4, Juan-José de-Damborenea5, Ramón Pérez-Tanoira6, Concepción Pérez-Jorge7, Jaime Esteban8. 1. Department of Clinical Microbiology, IIS-Fundación Jiménez Díaz, UAM. Av. Reyes Católicos 2, 28040 Madrid, Spain. Electronic address: martamp8@gmail.com. 2. Department of Surface Engineering Corrosion and Durability, National Center for Metallurgical Research, CENIM-CSIC, Avda. Gregorio del Amo, 8, 28040 Madrid, Spain. Electronic address: a.conde@cenim.csic.es. 3. Department of Surface Engineering Corrosion and Durability, National Center for Metallurgical Research, CENIM-CSIC, Avda. Gregorio del Amo, 8, 28040 Madrid, Spain. Electronic address: geles@cenim.csic.es. 4. Department of Statistics, IIS-Fundación Jiménez Díaz, UAM. Av. Reyes Católicos 2, 28040 Madrid, Spain. Electronic address: imahillo@fjd.es. 5. Department of Surface Engineering Corrosion and Durability, National Center for Metallurgical Research, CENIM-CSIC, Avda. Gregorio del Amo, 8, 28040 Madrid, Spain. Electronic address: jdambo@cenim.csic.es. 6. Department of Clinical Microbiology, IIS-Fundación Jiménez Díaz, UAM. Av. Reyes Católicos 2, 28040 Madrid, Spain. Electronic address: ramontanoira@hotmail.com. 7. Department of Clinical Microbiology, IIS-Fundación Jiménez Díaz, UAM. Av. Reyes Católicos 2, 28040 Madrid, Spain. Electronic address: cperemarch@fjd.es. 8. Department of Clinical Microbiology, IIS-Fundación Jiménez Díaz, UAM. Av. Reyes Católicos 2, 28040 Madrid, Spain. Electronic address: jestebanmoreno@gmail.com.
Abstract
OBJECTIVE: Implant-related infection is a devastating complication in orthopedic surgery. Aiming to minimize this problem, many material modifications have been developed. Here we report a study of a surface modification of Ti-6 Al-4 V alloy using a methodology that enables the study of interactions between bacteria and the material in the presence of eukaryotic cells. METHODS: We mixed different concentrations of collection or clinical strains of staphylococci isolated from implant-related infections with preosteoblastic cells using a previously published methodology, analyzing the minimal concentration of bacteria able to colonize the surface of the material through image analysis. Ti-6 Al-4 V alloy was modified by anodization to obtain two F-doped nanostructured surfaces that have been previously described to have antibacterial properties. RESULTS: Our results show similar bacterial adhesion results to nanoporous and nanotubular F-doped surfaces. The presence of preosteoblastic cells increases the adherence of all bacterial strains to both structures. No effect of the surface on eukaryotic cells adherence was detected. CONCLUSION: To our knowledge, this is the first time that anin vitro study emulating the race for the surface evaluates and compares the osseointegration and antibacterial properties between two nanostructured- modified titanium alloy surfaces. Clinical strains show different behavior from collection ones in bacterial adherence. The presence of cells increased bacterial adherence. NP and NT surface modifications didn´t show significant differences in bacterial adhesion and preosteoblastic cells integration.
OBJECTIVE: Implant-related infection is a devastating complication in orthopedic surgery. Aiming to minimize this problem, many material modifications have been developed. Here we report a study of a surface modification of Ti-6 Al-4 V alloy using a methodology that enables the study of interactions between bacteria and the material in the presence of eukaryotic cells. METHODS: We mixed different concentrations of collection or clinical strains of staphylococci isolated from implant-related infections with preosteoblastic cells using a previously published methodology, analyzing the minimal concentration of bacteria able to colonize the surface of the material through image analysis. Ti-6 Al-4 V alloy was modified by anodization to obtain two F-doped nanostructured surfaces that have been previously described to have antibacterial properties. RESULTS: Our results show similar bacterial adhesion results to nanoporous and nanotubular F-doped surfaces. The presence of preosteoblastic cells increases the adherence of all bacterial strains to both structures. No effect of the surface on eukaryotic cells adherence was detected. CONCLUSION: To our knowledge, this is the first time that anin vitro study emulating the race for the surface evaluates and compares the osseointegration and antibacterial properties between two nanostructured- modified titanium alloy surfaces. Clinical strains show different behavior from collection ones in bacterial adherence. The presence of cells increased bacterial adherence. NP and NT surface modifications didn´t show significant differences in bacterial adhesion and preosteoblastic cells integration.