Literature DB >> 30548893

Isolation and culture of human primary keratinocytes-a methods review.

Aneta Ścieżyńska1,2, Aleksandra Nogowska1, Maria Sikorska3,4, Justyna Konys1, Anita Karpińska2, Michał Komorowski2, Monika Ołdak1,5, Jacek Malejczyk1,2.   

Abstract

Keratinocyte culture is a necessary and widely used tool in a variety of experimental dermatological and biomedical studies. Literature search has revealed a variety of different protocols of human primary keratinocyte isolation and culture. Therefore, the aim of this paper was to review and summarize current trends in human primary keratinocyte culture techniques. We present data on the most popular and effective methods of human keratinocyte isolation and cultivation obtained from screening of 945 papers published during the last 10 years.
© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Entities:  

Keywords:  cell culture methods; cell isolation; cell proliferation; culture media; humans

Mesh：

Substances：

Year:  2019        PMID： 30548893     DOI： 10.1111/exd.13860

Source DB:  PubMed          Journal:  Exp Dermatol        ISSN： 0906-6705            Impact factor:   3.960


  10 in total

1.  Generating iPSCs with a High-Efficient, Non-Invasive Method-An Improved Way to Cultivate Keratinocytes from Plucked Hair for Reprogramming.

Authors:  Lisa S Wüstner; Moritz Klingenstein; Karl G Frey; Mohammad R Nikbin; Alfio Milazzo; Alexander Kleger; Stefan Liebau; Stefanie Klingenstein
Journal:  Cells       Date:  2022-06-17       Impact factor: 7.666

2.  Molecular mapping of interstitial lung disease reveals a phenotypically distinct senescent basal epithelial cell population.

Authors:  Daryle J DePianto; Jason A Vander Heiden; Katrina B Morshead; Kai-Hui Sun; Zora Modrusan; Grace Teng; Paul J Wolters; Joseph R Arron
Journal:  JCI Insight       Date:  2021-04-22

3.  Long-term expansion of directly reprogrammed keratinocyte-like cells and in vitro reconstitution of human skin.

Authors:  Jie Zheng; Wonjin Yun; Junghyun Park; Phil Jun Kang; Gilju Lee; Gwonhwa Song; In Yong Kim; Seungkwon You
Journal:  J Biomed Sci       Date:  2020-04-20       Impact factor: 8.410

4.  Cytotoxicity and Epidermal Barrier Function Evaluation of Common Antiseptics for Clinical Use in an Artificial Autologous Skin Model.

Authors:  María I Quiñones-Vico; Ana Fernández-González; Elena Pérez-Castejón; Trinidad Montero-Vílchez; Salvador Arias-Santiago
Journal:  J Clin Med       Date:  2021-02-08       Impact factor: 4.241

5.  Xeno-free workflow exhibits comparable efficiency and quality of keratinocytes isolated from human skin biopsies.

Authors:  Cathrine Lagerwall; Hady Shahin; Sallam Abdallah; Ingrid Steinvall; Moustafa Elmasry; Folke Sjöberg; Ahmed T El-Serafi
Journal:  Regen Ther       Date:  2021-09-29       Impact factor: 3.419

6.  Upregulated Guanine Deaminase Is Involved in Hyperpigmentation of Seborrheic Keratosis via Uric Acid Release.

Authors:  Kyung Ah Cheong; In Sup Kil; Hyuk Wan Ko; Ai-Young Lee
Journal:  Int J Mol Sci       Date:  2021-11-19       Impact factor: 5.923

7.  Fostering a healthy culture: Biological relevance of in vitro and ex vivo skin models.

Authors:  Scott X Atwood; Maksim V Plikus
Journal:  Exp Dermatol       Date:  2021-02-10       Impact factor: 3.960

8.  Molecular Analysis of the ABCA4 Gene Mutations in Patients with Stargardt Disease Using Human Hair Follicles.

Authors:  Aneta Ścieżyńska; Marta Soszyńska; Michał Komorowski; Anna Podgórska; Natalia Krześniak; Aleksandra Nogowska; Martyna Smolińska; Kamil Szulborski; Jacek P Szaflik; Bartłomiej Noszczyk; Monika Ołdak; Jacek Malejczyk
Journal:  Int J Mol Sci       Date:  2020-05-13       Impact factor: 5.923

9.  Immortalization and Characterization of Rat Lingual Keratinocytes in a High-Calcium and Feeder-Free Culture System Using ROCK Inhibitor Y-27632.

Authors:  Zixing Chen; Wenmeng He; Thomas Chun Ning Leung; Hau Yin Chung
Journal:  Int J Mol Sci       Date:  2021-06-24       Impact factor: 5.923

10.  A Novel and Effective Method for Human Primary Skin Melanocytes and Metastatic Melanoma Cell Isolation.

Authors:  Aneta Ścieżyńska; Anna Sobiepanek; Patrycja D Kowalska; Marta Soszyńska; Krzysztof Łuszczyński; Tomasz M Grzywa; Natalia Krześniak; Agata Góźdź; Paweł K Włodarski; Ryszard Galus; Tomasz Kobiela; Jacek Malejczyk
Journal:  Cancers (Basel)       Date:  2021-12-13       Impact factor: 6.639
  10 in total

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