Literature DB >> 30546166

Volumetric Investigations on Molecular Interactions of Glycine/l-alanine in Aqueous Citric Acid Solutions at Different Temperatures.

Poonam Patyar1, Gurpreet Kaur1, Tarnveer Kaur1.   

Abstract

Apparent molar volumes ( ϕ V ) of glycine/l-alanine in water and in aqueous citric acid (CA) solutions of varying concentrations, i.e. (0.05, 0.10, 0.20, 0.30, 0.40 and 0.50) mol·kg-1 were determined from density measurements at temperatures T = (288.15, 298.15, 308.15, 310.15 and 318.15) K and at atmospheric pressure. Limiting partial molar volumes ( ϕ V o ) and their corresponding partial molar volumes of transfer ( Δ tr ϕ V ) have been calculated from the ϕ V data. The negative Δ tr ϕ V values obtained for glycine/l-alanine from water to aqueous CA solutions indicate the dominance of hydrophilic-hydrophobic/hydrophobic-hydrophilic and hydrophobic-hydrophobic interactions over ion/hydrophilic-dipolar interactions. Further, pair and triplet interaction coefficients, i.e. ( V AB ) and ( V ABB ) along with hydration number ( n H ) have also been calculated. The effect of temperature on the volumetric properties of glycine/l-alanine in water and in aqueous CA solutions has been determined from the limiting partial molar expansibilities ( ∂ ϕ V o / ∂ T ) p and their second-order derivative ( ∂ 2 ϕ V o / ∂ T 2 ) P . The apparent specific volumes ( ν ϕ ) for glycine and l-alanine tend to approach sweet taste behavior both in the presence of water and in aqueous CA solutions. The ν ϕ values for glycine/l-alanine increase with increase in concentration of CA at all temperatures studied. This reveals that CA helps in enhancing the sweet taste behavior of glycine/l-alanine which also supports the dominance of hydrophobic-hydrophobic interactions.

Entities:  

Keywords:  Apparent specific volumes; Hydration number; Interaction coefficients; Partial molar expansibilities; Partial molar volumes; Partial molar volumes of transfer

Year:  2018        PMID： 30546166      PMCID： PMC6267150          DOI： 10.1007/s10953-018-0829-6

Source DB:  PubMed          Journal:  J Solution Chem        ISSN： 0095-9782            Impact factor:   1.677


Introduction

Amino acids are the building blocks of proteins, thus they are regarded as an ideal model for the study of protein functioning and their complex structure [1, 2]. The functional properties of protein molecules depend upon their three dimensional structure which arises due to a particular sequence of amino acids (hereafter written as AA) in a polypeptide chain. All AAs exists as zwitterionic species in aqueous solutions [3], thus their thermodynamic properties in a variety of media can provide valuable information about the stability and denaturation of proteins [4-16]. Recently, Lomesh and Kumar [17, 18] have reported the volumetric and acoustic properties of glycine, diglycine, l-alanine and l-phenylalanine in water and in 0.1 mol·kg−1 aqueous citric acid at different temperatures. Further, a literature survey reveals that not much systematic data is available regarding the thermodynamic and transport properties of AA as a function of concentration in citric acid solutions at different temperatures. Citric acid (CA) (2-hydroxy-1,2,3-propanetricarboxylic acid) is a tri-basic acid and common metabolite of plants and animals. It is an environmentally acceptable organic acid, used in food, beverages, pH adjustment in buffers, and pharmaceuticals (as an acidifier) [19, 20], and is known to increase the stability of proteins [13]. The presence of one hydroxyl and three carboxyl groups in CA provides effective chemical properties so that it can act as an important metabolite in the citric acid cycle (CAC) of all aerobic organisms [21]. It also acts as a precursor for the bio-synthesis of many compounds in CAC including AA [22]. Therefore, it is of great interest to investigate the molecular interactions of AA with CA which can influence the behavior and conformational stability of proteins. In light of the above facts, presently we report the apparent molar volumes of glycine/l-alanine (both are non-essential amino acids) in water and in aqueous CA solutions, (molality of aqueous CA) ≈ (0.05, 0.10, 0.20, 0.30, 0.40 and 0.50) mol·kg−1 at temperatures, T = (288.15, 298.15, 308.15, 310.15 and 318.15) K and at atmospheric pressure, obtained from experimental densities. Partial molar volumes calculated from data have been used to calculate partial molar volumes of transfer apparent specific volumes , pair and triplet interaction coefficients, partial molar expansibilities , their second order derivatives and hydration number . The volumetric behavior of glycine in aqueous CA solutions (present work) are compared with glycine in aqueous succinic acid (SA) solutions, reported earlier from our laboratory [23].

Experimental Section

Chemicals Used

Glycine (C2H5NO2), l-alanine (C3H7NO2) and citric acid (C6H8O7) of analytical grade with mass fraction purity ≥ 99% were procured from S. D. Fine Chemical Ltd. (SDFCL), India. Specifications of the chemicals used are given in Table 1. All the chemicals were used without any further purification; however, they were dried in a vacuum oven for 24 h at T = 318.15 K, and then kept in a vacuum desiccator over anhydrous CaCl2 prior to their use.
Table 1

Specifications of the chemicals used in present work

Chemical nameMolecular formulaStructureMolecular weight (\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$10^{ - 3}$$\end{document}10-3, kg·\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\text{mol}}^{ - 1}$$\end{document}mol-1)CAS no.SourceMass fraction purity (%)
Glycine \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\text{C}}_{2} {\text{H}}_{5} {\text{NO}}_{2}$$\end{document}C2H5NO2 75.0756-40-6SDFCL≥ 99
l-Alanine \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\text{C}}_{3} {\text{H}}_{7} {\text{NO}}_{2}$$\end{document}C3H7NO2 89.0956-41-7SDFCL≥ 99
Ctric acid, anhydrous \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\text{C}}_{6} {\text{H}}_{8} {\text{O}}_{7}$$\end{document}C6H8O7 192.1377-92-9SDFCL≥ 99
Specifications of the chemicals used in present work Deionized, double distilled and degassed water with specific conductance < 1 × 10−4 S·m−1 was used to prepare all the solutions. The pHs of the experimental solutions were checked using a pH meter (Systronics digital pH meter-335, India). The standard deviation obtained for the whole set of experimental data is ± 0.02 pH unit. Accuracy in pH measurements was checked by calibrating the pH meter using standard buffer solutions of pH 7.00 and pH 9.20. The pHs of the stock solutions, i.e. at all concentrations of aqueous citric acid solutions, lie between 2.08 and 2.44, in the case of glycine in aqueous citric acid solutions the pHs lie from 1.96 to 3.61 and for l-alanine in aqueous citric acid solutions it varies from 1.99 to 3.94. Solutions were prepared on the molality basis using a Citizen CY-204 balance having a precision of ± 0.1 mg. The overall uncertainty in molality was estimated to be < 5 × 10−3 mol·kg−1. Solution densities were measured using a vibrating-tube digital density meter (DMA 4500 M from Anton Paar, Austria). The sensitivity of the instrument corresponds to a precision in density measurements of ± 1 × 10−2 kg·m−3 and accuracy of ± 5 × 10−2 kg·m−3, respectively. The density meter has a built in thermostat to maintain the desired temperatures within ± 0.01 K and was calibrated with double distilled and degassed water before each series of experiments. The performance of the density meter was checked by measuring the densities of aqueous sodium chloride (NaCl) solutions, which agree well with the literature values [24] as shown in Fig. 1.
Fig. 1

Representative plots of densities versus molality : (blue filled square, present work; red filled square, literature values [24] of NaCl solutions at 288.15 K (Color figure online)

Representative plots of densities versus molality : (blue filled square, present work; red filled square, literature values [24] of NaCl solutions at 288.15 K (Color figure online)

Results and Discussion

Volumetric Properties

The apparent molar volumes of glycine and l-alanine in water and in varying concentrations of aqueous CA solutions were calculated from experimental solution densities at temperatures T = (288.15, 298.15, 308.15, 310.15 and 318.15) K and at atmospheric pressure, by employing the following equation:where M (kg·mol−1) is the molar mass of the glycine/l-alanine, mA (mol·kg−1) is the molality of glycine/l-alanine, and are the densities of the solvent (water or water + CA) and solution (water + CA + glycine/l-alanine), respectively. The values of glycine/l-alanine along with and as a function of molality, in water and in aqueous CA solutions at different temperatures, are summarized in Table 2. The standard uncertainty in the apparent molar volume due to molality u(m) and density u(ρ) has been calculated and is (≤ 0.0960 and ≤ 1.611 × 10−6 m3·mol−1), respectively. Representative plots of values for the presently studied systems (i.e. glycine, l-alanine and citric acid in water) at T = 288.15 K agree well with earlier reported data [25-34] and are shown in Figs. 2, 3 and 4. The values reported by the authors [25, 33] are higher (at lower concentrations) than to our present results as well as with the other literature data. (The standard uncertainty in molality, u(m) and apparent molar volume, u() of (succinic acid + glycine) system are ≤ 2.21 × 10−4 mol·kg−1 and (0.03–0.65) × 10−6 m3·mol−1, respectively).
Table 2

Densities and apparent molar volumes of glycine and l-alanine in water and in aqueous CA solutions at T = (288.15, 298.15, 308.15, 310.15 and 318.15) K and p = 101.3 kPa

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Glycine in water
 0.00000.999090.997040.994030.993320.99021
 0.10781.0025942.461.0004643.220.9973843.910.9966743.920.9935344.25
 0.19911.0055142.561.0033143.341.0001843.990.9994744.000.9963144.29
 0.29111.0084142.671.0061443.451.0029644.081.0022444.130.9990744.38
 0.40341.0118942.811.0095443.581.0063244.141.0055944.201.0023944.47
 0.52821.0156942.941.0132643.691.0100044.211.0092644.281.0060244.57
 0.67911.0202243.061.0176943.801.0143744.301.0136244.371.0103544.66
 0.82441.0244843.181.0218643.911.0184944.391.0177344.461.0144344.75
 0.95041.0280743.331.0253944.041.0219944.501.0211944.601.0175644.89
Glycine in 0.05 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left( {{\text{mol}}{\cdot}{\text{kg}}^{ - 1} } \right)$$\end{document}mol·kg-1 CA
 0.00001.002371.000230.997150.996430.99328
 0.19811.0087642.521.0064743.301.0033043.791.0025543.950.9993544.25
 0.30221.0120542.621.0096743.421.0064643.891.0057044.041.0024744.35
 0.41961.0157042.701.0132243.541.0099743.991.0091944.141.0059344.45
 0.50621.0183542.791.0158143.611.0124844.151.0117244.231.0084444.54
 0.61031.0214942.891.0188643.731.0155044.231.0147344.331.0114244.64
 0.71791.0247042.981.0220043.791.0185944.301.0178344.361.0144844.69
 0.75991.0259143.051.0231943.851.0197644.361.0190044.421.0156644.72
 0.85381.0295643.161.0267743.931.0233044.421.0225144.511.0191244.82
Glycine in 0.10 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left( {{\text{mol}}{\cdot}{\text{kg}}^{ - 1} } \right)$$\end{document}mol·kg-1 CA
 0.00001.007291.005031.001861.001110.99790
 0.22581.0145842.391.0121743.241.0088643.731.0081043.791.0048444.05
 0.30801.0171942.431.0146543.361.0113643.821.0105843.891.0072844.23
 0.41541.0205342.561.0179143.441.0145443.941.0137844.001.0104644.31
 0.52211.0238042.661.0211043.531.0176944.031.0168944.131.0135744.39
 0.61591.0266542.731.0238343.661.0203844.161.0196044.221.0162444.51
 0.72951.0300542.811.0271543.711.0236244.251.0228344.321.0194344.63
 0.83881.0332442.931.0302743.811.0266944.341.0259144.391.0224644.73
 0.93711.0360943.011.0330343.891.0299444.391.0286044.491.0251544.80
Glycine in 0.20 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left( {{\text{mol}}{\cdot}{\text{kg}}^{ - 1} } \right)$$\end{document}mol·kg-1 CA
 0.00001.015231.012771.009431.008661.00533
 0.08591.0180342.191.0155242.781.0121443.281.0113743.291.0080343.44
 0.18911.0213642.241.0187842.891.0153543.401.0145743.461.0112143.60
 0.31021.0252042.341.0225143.101.0190343.581.0182543.621.0148743.83
 0.40661.0281942.481.0254043.301.0219143.701.0211243.811.0176944.05
 0.52071.0316942.581.0288043.421.0252243.921.0244144.011.0209744.26
 0.55061.0325642.681.0296843.461.0260843.971.0252744.051.0218244.31
 0.69851.0370042.781.0339343.671.0302644.181.0294544.251.0259844.50
 0.80411.0401142.871.0369543.761.0332244.291.0324044.361.0289244.60
 0.91821.0434242.961.0401643.851.0363944.371.0355944.441.0320244.72
Glycine in 0.30 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left( {{\text{mol}}{\cdot}{\text{kg}}^{ - 1} } \right)$$\end{document}mol·kg-1 CA
 0.00001.023291.020641.017101.016311.01289
 0.10081.0265842.061.0238842.571.0203042.991.0194943.191.0160543.43
 0.19341.0295642.151.0267642.721.0231743.221.0223543.381.0189043.57
 0.26061.0316942.231.0288242.891.0252143.381.0243843.541.0209243.73
 0.36471.0349542.341.0319643.061.0283543.511.0274943.701.0240143.91
 0.52811.0399942.471.0368043.211.0331643.721.0323243.821.0287144.22
 0.59441.0419942.541.0386643.321.0350343.881.0341943.971.0306144.28
 0.65691.0438642.601.0404243.461.0367744.011.0359244.111.0323444.40
 0.73371.0461442.671.0426043.581.0388744.181.0380544.231.0344544.52
 0.89011.0507342.771.0472343.741.0432244.301.0423844.371.0387344.68
Glycine in 0.40 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left( {{\text{mol}}{\cdot}{\text{kg}}^{ - 1} } \right)$$\end{document}mol·kg-1 CA
 0.00001.031791.028941.025251.024431.02089
 0.31181.0419241.831.0389842.141.0351942.481.0342442.891.0306243.18
 0.43411.0457442.011.0427942.261.0389842.561.0379842.971.0343243.28
 0.51221.0481442.111.0451842.341.0413842.591.0403243.051.0366443.36
 0.58201.0502342.241.0472742.451.0435042.631.0423943.121.0386943.43
 0.69921.0537342.381.0507942.541.0470342.681.0458443.201.0421243.50
 0.72541.0544742.451.0515542.581.0477942.721.0465943.241.0428543.55
 0.92711.0603542.641.0574142.771.0537242.821.0554143.341.0486543.62
Glycine in 0.50 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left( {{\text{mol}}{\cdot}{\text{kg}}^{ - 1} } \right)$$\end{document}mol·kg-1 CA
 0.00001.042931.039881.036011.035161.03150
 0.09811.0461741.501.0430941.811.0391842.331.0383042.531.0345943.05
 0.20341.0495941.611.0464741.961.0425142.421.0416042.611.0378443.22
 0.29781.0526141.711.0494542.121.0454742.481.0445642.681.0404643.39
 0.41301.0562241.861.0529742.351.0490942.531.0481042.751.0441143.55
 0.49881.0588741.961.0555442.521.0516442.621.0507042.801.0466243.65
 0.61041.0622542.101.0588542.671.0549942.711.0540242.901.0498243.79
 0.66781.0639742.161.0605242.761.0566942.761.0557142.951.0514443.87
l-Alanine in water
 0.00000.999090.997040.994030.993320.99021
 0.10781.0022459.711.0001260.430.9970760.900.9963561.020.9932361.22
 0.18181.0043659.811.0022060.480.9991260.970.9984061.050.9952761.27
 0.31141.0080259.911.0058060.521.0026761.021.0019461.100.9987761.41
 0.40771.0106959.971.0084160.611.0052561.081.0045261.161.0013261.49
 0.71491.0189260.191.0164960.801.0132361.261.0124761.361.0092461.64
 0.81301.0214460.281.0189660.891.0156761.351.0149161.441.0116661.73
 0.89861.0236360.331.0211260.921.0177961.401.0170461.471.0136961.85
l-Alanine in 0.05 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left( {{\text{mol}}{\cdot}{\text{kg}}^{ - 1} } \right)$$\end{document}mol·kg-1 CA
 0.00001.004841.002680.999580.998850.99569
 0.13301.0087159.611.0065459.761.0034459.851.0027059.950.9995360.12
 0.19731.0105459.711.0083759.831.0052759.931.0045360.001.0013560.20
 0.26311.0123959.801.0102159.941.0071160.031.0063760.101.0031860.31
 0.40411.0162859.941.0140760.131.0109860.211.0102360.281.0070560.43
 0.49401.0187160.021.0164860.231.0133960.311.0126360.401.0094560.54
 0.58001.0210060.101.0187460.341.0156660.411.0148960.501.0117360.60
 0.69711.0240660.201.0217760.461.0186760.561.0179160.631.0147660.72
 0.78331.0262660.291.0239660.541.0208860.621.0201160.701.0169360.83
 0.90421.0293360.371.0269660.671.0238760.761.0225560.821.0199460.94
l-Alanine in 0.10 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left( {{\text{mol}}{\cdot}{\text{kg}}^{ - 1} } \right)$$\end{document}mol·kg-1 CA
 0.00001.009781.007521.004331.003581.00036
 0.10731.0129059.531.0106459.601.0074559.701.0066959.811.0034759.91
 0.19241.0153359.611.0130759.681.0098759.831.0091159.911.0058860.06
 0.29941.0183359.721.0160659.831.0128859.891.0121060.021.0088660.19
 0.39701.0210159.821.0187559.891.0155560.021.0147860.091.0115160.32
 0.49551.0236659.931.0213960.021.0182060.131.0174260.211.0141460.44
 0.60091.0264560.031.0241860.121.0209660.271.0202060.311.0169260.52
 0.70621.0292060.101.0269260.211.0237160.341.0229360.401.0196560.60
 0.81291.0319060.221.0296360.311.0264360.421.0256260.521.0223460.70
 0.95391.0354260.331.0331560.421.0299360.561.0291560.611.0257760.89
l-Alanine in 0.20 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left( {{\text{mol}}{\cdot}{\text{kg}}^{ - 1} } \right)$$\end{document}mol·kg-1 CA
 0.00001.017811.015351.011971.011201.00789
 0.09901.0206859.391.0182159.561.0148359.671.0140659.691.0107559.80
 0.18441.0231059.531.0206359.661.0172559.761.0164859.791.0131659.94
 0.31281.0266959.611.0242059.781.0208359.851.0200559.911.0167060.14
 0.38731.0287259.701.0262359.851.0228659.931.0220760.011.0187160.24
 0.49351.0315759.811.0290959.931.0257160.041.0249160.121.0215560.33
 0.59621.0342859.901.0317760.061.0284160.131.0275860.261.0242460.42
 0.70441.0370859.991.0345860.121.0312160.221.0303760.341.0269960.55
 0.78441.0390660.121.0366060.211.0332160.331.0323960.421.0290060.63
 0.89321.0417760.221.0392960.321.0359160.431.0350660.551.0316960.73
l-Alanine in 0.30 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left( {{\text{mol}}{\cdot}{\text{kg}}^{ - 1} } \right)$$\end{document}mol·kg-1 CA
 0.00001.026901.024241.020721.019911.01647
 0.08581.0293759.301.0267159.381.0231959.491.0223759.631.0189359.74
 0.18641.0322259.391.0295559.521.0260359.631.0252159.711.0217759.82
 0.28571.0349859.471.0323059.621.0287959.701.0279559.821.0245259.90
 0.41471.0384959.581.0358059.731.0323059.801.0314659.901.0280260.01
 0.52901.0415359.681.0388359.841.0353459.901.0344960.001.0310460.13
 0.61501.0437759.761.0410659.931.0375859.981.0367160.101.0332560.25
 0.70231.0460059.851.0432860.021.0398060.081.0389260.211.0354660.35
 0.78841.0481759.931.0454460.111.0419560.181.0410760.301.0375960.46
 0.84301.0495259.991.0467660.191.0432960.251.0423960.381.0389260.53
l-Alanine in 0.40 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left( {{\text{mol}}{\cdot}{\text{kg}}^{ - 1} } \right)$$\end{document}mol·kg-1 CA
 0.00001.034791.031931.028251.027411.02385
 0.17111.0396559.291.0367959.441.0331159.551.0322659.641.0287059.75
 0.29591.0431059.391.0402259.551.0365559.661.0356959.761.0321359.84
 0.37111.0451359.481.0422559.631.0385759.771.0377159.851.0341659.91
 0.48461.0481559.581.0452659.741.0415759.871.0407159.941.0371660.03
 0.57121.0503959.691.0475159.821.0438259.951.0429460.051.0393760.18
 0.70491.0538159.801.0509359.921.0472460.051.0463560.151.0427660.31
 0.91621.0590559.931.0561560.101.0524660.231.0515760.311.0479760.48
l-Alanine in 0.50 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left( {{\text{mol}}{\cdot}{\text{kg}}^{ - 1} } \right)$$\end{document}mol·kg-1 CA
 0.00001.041641.038601.034751.033891.03024
 0.08251.0439959.141.0409559.231.0371059.361.0362459.381.0325959.50
 0.18081.0467459.241.0437059.341.0398559.461.0389859.541.0353359.66
 0.28481.0495959.341.0465659.411.0427059.561.0418359.621.0381859.74
 0.38011.0521559.441.0491159.541.0452759.641.0443959.721.0407459.84
 0.49731.0552559.531.0522159.631.0483759.731.0474859.821.0438459.92
 0.60301.0579759.641.0549359.741.0511059.831.0502159.911.0465760.01
 0.70181.0604559.751.0573859.891.0535959.931.0526960.021.0490560.13
 0.78891.0625959.851.0595259.991.0557460.031.0548460.111.0511760.25
 0.82161.0633759.901.0603160.031.0565260.091.0555960.201.0519760.28

Standard uncertainties: u  = ≤ 5.86 ×  ,  ≤ 1.16 × ,  = 0.03 K,  = 0.5 kPa

Fig. 2

Representative plots of apparent molar volumes versus molality of glycine in water at 288.15 K: (red filled square, present work and blue filled square, literature values [23, 25–32]) (Color figure online)

Fig. 3

Representative plots of apparent molar volumes versus molality of l-alanine in water at 288.15 K: red filled square, present work and blue filled square, literature values [26, 29, 32, 33] (Color figure online)

Fig. 4

Representative plots of apparent molar volumes versus molality of citric acid in water at 288.15 K: red filled square, Present work and blue filled square literature values [34] (Color figure online)

Densities and apparent molar volumes of glycine and l-alanine in water and in aqueous CA solutions at T = (288.15, 298.15, 308.15, 310.15 and 318.15) K and p = 101.3 kPa Standard uncertainties: u  = ≤ 5.86 ×  ,  ≤ 1.16 × ,  = 0.03 K,  = 0.5 kPa Representative plots of apparent molar volumes versus molality of glycine in water at 288.15 K: (red filled square, present work and blue filled square, literature values [23, 25–32]) (Color figure online) Representative plots of apparent molar volumes versus molality of l-alanine in water at 288.15 K: red filled square, present work and blue filled square, literature values [26, 29, 32, 33] (Color figure online) Representative plots of apparent molar volumes versus molality of citric acid in water at 288.15 K: red filled square, Present work and blue filled square literature values [34] (Color figure online) The variation of versus for glycine/l-alanine in water and in different concentrations of aqueous CA solutions at 288.15 K are shown in Figs. 5 and 6, respectively (representative plots only). It is observed from Figs. 5 and 6 that the values of glycine/l-alanine in aqueous CA solutions vary almost linearly with increases in AA concentration as well as with temperature, whereas these values decrease with increases in the concentration of aqueous CA in these solutions. The higher values obtained for glycine/l-alanine in water indicate that strong solute–solvent interactions exist between glycine/l-alanine and water. In fact, strong interactions of the three carboxyl groups and one hydroxyl group of CA with water via hydrogen bonds leads to the higher values, however the hydrophobic group in SA [23] reduces its ability to form strong hydrogen bonds with water, thus resulting in smaller values (Fig. 7a). The basic structures of citric acid (CA), succinic acid (SA), glycine and l-alanine are given in Scheme 1.
Fig. 5

Representative plots of apparent molar volumes versus molality of glycine in water and in different concentrations of aqueous CA solutions at 288.15 K: (mc) = blue filled diamond, water; red filled square, 0.05; Green filled triangle, 0.10; Violet multiply symbol, 0.20; blue open diamond, 0.30; orange filled circle, 0.40; blue open triangle, 0.50 (Color figure online)

Fig. 6

Representative plots of apparent molar volumes versus molality of l-alanine in water and in different concentrations of aqueous CA solutions at 288.15 K:  = blue filled diamond, water; red filled square, 0.05; Green filled triangle, 0.10; Violet multiply symbol, 0.20; blue open diamond, 0.30; orange filled circle, 0.40; blue open triangle, 0.50 (Color figure online)

Fig. 7

(a) Representative plots of apparent molar volumes versus molality of glycine in: blue filled diamond, 0.10 aqueous CA solutions and red filled square, 0.10 in aqueous succinic acid (abbreviated as SA) solutions at 288.15 K. b Plots of apparent molar volumes of glycine versus molality in 0.10 aqueous succinic acid solutions (abbreviated as SA) at different temperatures, T = (288.15–328.15) K (Color figure online)

Scheme 1

Basic structures of (a) citric acid, (b) succinic acid, (c) glycine and (d) l-alanine

Representative plots of apparent molar volumes versus molality of glycine in water and in different concentrations of aqueous CA solutions at 288.15 K: (mc) = blue filled diamond, water; red filled square, 0.05; Green filled triangle, 0.10; Violet multiply symbol, 0.20; blue open diamond, 0.30; orange filled circle, 0.40; blue open triangle, 0.50 (Color figure online) Representative plots of apparent molar volumes versus molality of l-alanine in water and in different concentrations of aqueous CA solutions at 288.15 K:  = blue filled diamond, water; red filled square, 0.05; Green filled triangle, 0.10; Violet multiply symbol, 0.20; blue open diamond, 0.30; orange filled circle, 0.40; blue open triangle, 0.50 (Color figure online) (a) Representative plots of apparent molar volumes versus molality of glycine in: blue filled diamond, 0.10 aqueous CA solutions and red filled square, 0.10 in aqueous succinic acid (abbreviated as SA) solutions at 288.15 K. b Plots of apparent molar volumes of glycine versus molality in 0.10 aqueous succinic acid solutions (abbreviated as SA) at different temperatures, T = (288.15–328.15) K (Color figure online) Basic structures of (a) citric acid, (b) succinic acid, (c) glycine and (d) l-alanine Solute–solute interactions are eliminated at infinite dilution and hence the apparent molar volume becomes equal to the limiting partial molar volume . The values have been evaluated by least-squares fitting of the following equation to the corresponding data:where provides a measure of solute–solvent interactions, and the experimental slope provides information regarding solute–solute interactions [28]. and values of glycine/l-alanine in water and in aqueous CA solutions at different temperatures are listed in Table 3. The standard uncertainty of the limiting partial molar volumes has been found to be ≤ 0.07 × 10−6 m3·mol−1. The values of glycine/l-alanine in water agree well with the literature values at different temperatures and are shown in Figs. 8 and 9 [8, 23, 26–28, 30, 35, 36]. Table 3 shows that the values are higher for l-alanine in water and in aqueous CA solutions compared to glycine, as expected, which is due to the increase in the molar mass of l-alanine. The magnitude of the values is higher than the Sv values, which suggests that the extent of solute–solvent interactions is greater compared to solute–solute interactions. Moreover, the values decrease with increase in aqueous CA concentration whereas they increase with increasing temperature. The decrease in values with increasing concentration of CA may be attributed to the disruption of side group hydration by that of the charged end groups (a similar explanation has been given by Wang et al. [37], which supports our results for the values), whereas the increase in values of glycine/l-alanine in CA solutions with increase in temperature may lead to reduction of the electrostriction around the zwitterions [38]. Also, at higher temperatures, solvent from the secondary solvation layer of glycine/l-alanine is released into the bulk of solvent which results in the expansion of the solution and leads to higher values [39].
Table 3

Limiting partial molar volumes of glycine/l-alanine in water and in aqueous CA solutions at T = (288.15, 298.15, 308.15, 310.15 and 318.15) K

\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$m_{\text{c}}$$\end{document}mc (mol·kg−1) \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$10^{6} \times \phi _{V}^{{\text{o}}} \,({\text{m}}^{3} {\cdot}{\text{mol}}^{{ - 1}} )$$\end{document}106×ϕVo(m3·mol-1)
288.15, T (K)298.15, T (K)308.15, T (K)310.15, T (K)318.15, T (K)
Glycine
 0.0042.37a ± 0.02(1.01)b[0.99]c42.69d,42.37e, 42.38f43.16a ± 0.03(0.93)[0.99]43.51d, 43.27e, 42.28f 43.16g,43.86a ± 0.02(0.66)[0.99]43.77d, 43.98e, 43.76f 43.87h43.87a ± 0.03(0.76)[0.99]44.10d44.16a ± 0.02(0.75)[0.99]44.43d, 44.16f 44.17i, 44.14j
 0.0542.32 ± 0.01(0.92)[0.99]43.14 ± 0.02(0.92)[0.99]43.61 ± 0.03(0.96)[0.99]43.80 ± 0.02(0.81)[0.99]44.10 ± 0.02(0.82)[0.99]
 0.1042.18 ± 0.01(0.82)[0.99]43.07 ± 0.02(0.89)[0.99]43.54 ± 0.02(0.95)[0.98]43.60 ± 0.02(0.97)[0.99]43.87 ± 0.03(1.02)[0.99]
 0.2042.08 ± 0.03[0.99][0.99]42.69 ± 0.05(1.34)[0.99]43.16 ± 0.04(1.39)[0.99]43.20 ± 0.05(1.44)[0.99]43.35 ± 0.07(1.59)[0.99]
 0.3041.98 ± 0.02(0.92)[0.99]42.46 ± 0.04(1.48)[0.99]42.89 ± 0.05(1.66)[0.99]43.10 ± 0.05(1.48)[0.99]43.29 ± 0.04(1.65)[0.99]
 0.4041.43 ± 0.03(1.35)[0.99]41.82 ± 0.02(1.04)[0.99]42.31 ± 0.01(0.54)[0.99]42.66 ± 0.02(0.77)[0.99]42.97 ± 0.03(0.74)[0.99]
 0.5041.37 ± 0.01(1.18)[0.99]41.63 ± 0.02(1.72)[0.99]42.26 ± 0.02(0.73)[0.99]42.46 ± 0.01(0.72)[0.99]42.94 ± 0.02(1.42)[0.99]
l-Alanine
 0.0059.66a ± 0.02(0.76)[0.99]59.67e60.35a ± 0.02(0.64)[0.99]60.42e60.84a ± 0.02(0.62)[0.99]60.88e60.94a ± 0.02(0.59)[0.99]61.15a ± 0.03(0.74)[0.99]
 0.0559.53 ± 0.02(0.97)[0.99]59.62 ± 0.02(1.19)[0.99]59.71 ± 0.02(1.18)[0.99]59.80 ± 0.02(1.16)[0.99]60.00 ± 0.02(1.04)[0.99]
 0.1059.44 ± 0.01(0.96)[0.99]59.51 ± 0.02(0.98)[0.99]59.62 ± 0.03(1.01)[0.99]59.72 ± 0.02(0.96)[0.99]59.85 ± 0.03(1.09)[0.99]
 0.2059.31 ± 0.02(1.01)[0.99]59.48 ± 0.01(0.94)[0.99]59.57 ± 0.01(0.95)[0.99]59.59 ± 0.01(1.08)[0.99]59.74 ± 0.03(1.14)[0.99]
 0.3059.21 ± 0.01(0.90)[0.99]59.31 ± 0.02(1.02)[0.99]59.42 ± 0.02(0.95)[0.99]59.52 ± 0.03(0.97)[0.99]59.61 ± 0.03(1.05)[0.99]
 0.4059.13 ± 0.02(0.92)[0.99]59.26 ± 0.02(0.94)[0.99]59.36 ± 0.03(0.98)[0.99]59.44 ± 0.03(0.99)[0.99]59.52 ± 0.03(1.09)[0.99]
 0.5059.05 ± 0.01(1.00)[0.99]59.12 ± 0.02(1.08)[0.99]59.28 ± 0.02(0.95)[0.99]59.32 ± 0.03(1.02)[0.99]59.44 ± 0.03(1.00)[0.99]

aPresent work

b 106 values in parenthesis ()

cRegression coefficient values in square brackets []

dReference [23]

eReference [26]

fReference [8]

gReference [27]

hReference [35]

iReference [36]

jReference [28]

The standard uncertainties in molality, u is ≤ 5.86

The standard uncertainty in temperature, is 0.03 K

The standard uncertainty in pressure, is 0.5 kPa

The standard uncertainty in partial molar volume, u is ≤ 0.07 × 

± Respective errors in values

Fig. 8

Representative plots of limiting partial molar volumes versus molality of glycine in: blue filled diamond, aqueous CA solutions and red filled square, in aqueous SA solutions at 288.15 K (Color figure online)

Fig. 9

Comparative plots of limiting partial molar volumes of glycine in water versus temperature, T = (288.15–318.15) K

Limiting partial molar volumes of glycine/l-alanine in water and in aqueous CA solutions at T = (288.15, 298.15, 308.15, 310.15 and 318.15) K aPresent work b 106 values in parenthesis () cRegression coefficient values in square brackets [] dReference [23] eReference [26] fReference [8] gReference [27] hReference [35] iReference [36] jReference [28] The standard uncertainties in molality, u is ≤ 5.86 The standard uncertainty in temperature, is 0.03 K The standard uncertainty in pressure, is 0.5 kPa The standard uncertainty in partial molar volume, u is ≤ 0.07 × ± Respective errors in values Representative plots of limiting partial molar volumes versus molality of glycine in: blue filled diamond, aqueous CA solutions and red filled square, in aqueous SA solutions at 288.15 K (Color figure online) Comparative plots of limiting partial molar volumes of glycine in water versus temperature, T = (288.15–318.15) K Comparison of and values of glycine in CA (present work) with previously reported data of glycine in SA [23] reveals that the magnitudes of and values for glycine–CA are higher than for glycine–SA (Figs. 7(a), (b), 10). As discussed earlier, the higher magnitudes for glycine in CA solutions are again attributed to the presence of additional hydrophilic groups (–OH and –COOH in CA) which leads to stronger interactions between glycine/l-alanine and CA, i.e. it is due to the formation of hydrogen bonds (Scheme 2) [40]. Further, the effect of pH on speciation and charge distribution of these systems involves the stronger interaction among citrate ion and the ionized ammonium group. The amino and carboxyl groups of glycine/l-alanine dissociate in aqueous citric acid solutions and form negatively and positively charged ions (i.e., +NH3-(CH(H/CH3)-COO–). Dissociation of citric acid in aqueous solutions [19], i.e. the negatively and positively charged ions, results in the formation of new species in aqueous solutions as:
Fig. 10

Comparative plots of limiting partial molar volumes of l-alanine in water versus temperature, T = (288.15–318.15) K

Scheme 2

Different types of possible interactions (i) glycine–CA and (ii) l-alanine–CA at pH 2.12 and at T = 310.15 K

Comparative plots of limiting partial molar volumes of l-alanine in water versus temperature, T = (288.15–318.15) K Different types of possible interactions (i) glycine–CA and (ii) l-alanine–CA at pH 2.12 and at T = 310.15 K Partial molar volumes of transfer of glycine/l-alanine from water to aqueous CA solutions at infinite dilution have been calculated by using the following equation: The values reported in Table S1 (supplementary material) are negative and decrease with increase in the concentration of aqueous CA solutions at the studied temperatures (Figs. 11 and 12). The standard uncertainty in values has been found to be ≤ 0.07 × 10−6 m3·mol−1. The possible interactions which may exist between ternary system (i.e. glycine/l-alanine + aqueous CA) (Scheme 2) can be categorized as: (1) ion/hydrophilic–dipolar interactions between (3COO–, -OH) of CA and (NH3+, COO–) zwitterions of glycine/l-alanine, (2) hydrophilic–hydrophobic interactions between the (3COO–, -OH) groups of CA with the non-ionic group of glycine/l-alanine, (3) hydrophobic–hydrophilic interactions between the non-ionic group of CA and zwitterionic groups of glycine/l-alanine, and (4) hydrophobic–hydrophobic group interactions between the alkyl chains of glycine/l-alanine and CA. According to the co-sphere overlap model [41], the overlap of the hydration co-spheres of hydrophilic and ionic parts (type 1) results in positive values, whereas interactions of type 2, 3 and 4 result in negative transfer volumes. The presently observed negative values for glycine/l-alanine in aqueous CA solutions at different temperatures suggest the dominance of hydrophilic–hydrophobic and hydrophobic–hydrophobic interactions over ion/hydrophilic–dipolar interactions. The greater magnitude of observed in the case of l-alanine in CA may be attributed to the presence of an alkyl group in l-alanine which further strengthens the view that hydrophobic–hydrophobic interactions are dominating in the case of l-alanine–CA (i.e. type 4 interactions).
Fig. 11

Plots of partial molar volumes of transfer versus molalities of CA of glycine at different temperatures: T = blue filled diamond, 288.15, red filled square, 298.15; Green filled triangle, 308.15; Violet open diamond, 310.15; blue open square, 318.15 K (Color figure online)

Fig. 12

Plots of partial molar volumes of transfer versus molalities of CA of l-alanine at different temperatures: T = blue filled diamond, 288.15; red filled square, 298.15; Green filled triangle, 308.15; Violet open diamond, 310.15; blue open square, 318.15 K (Color figure online)

Plots of partial molar volumes of transfer versus molalities of CA of glycine at different temperatures: T = blue filled diamond, 288.15, red filled square, 298.15; Green filled triangle, 308.15; Violet open diamond, 310.15; blue open square, 318.15 K (Color figure online) Plots of partial molar volumes of transfer versus molalities of CA of l-alanine at different temperatures: T = blue filled diamond, 288.15; red filled square, 298.15; Green filled triangle, 308.15; Violet open diamond, 310.15; blue open square, 318.15 K (Color figure online) The magnitude of values of glycine/l-alanine in CA can also be explained by considering the modified equation of Shahidi and Farrell [42],where is the van der Waal’s volume, is the volume associated with voids, and is the volume due to shrinkage that arises from electrostriction of solvent molecules caused by hydrophilic groups present in the solute. Assuming that and are not significantly affected by the presence of CA, then the negative values may be attributed to enhanced electrostriction in the vicinity of charged centers of zwitterions which results in an increase of . Further, it is observed from Table S1 that the values for glycine in CA are higher in contrast to glycine in SA solutions [23], which is attributed to the presence of additional hydrophilic groups in CA that leads to the formation of strong hydrogen bonding with glycine (Scheme 2). The McMillan–Mayer theory of solutions [43, 44] permits the formal separation of the effects due to the interactions between two or more solutes. According to this theory, the pair and triplet interaction coefficients (VAB) and (VABB) can be calculated from the partial molar volumes of transfer by using the following equation:where A denotes glycine/l-alanine, B denotes CA and is the molality of CA. The VAB and VABB values of glycine/l-alanine in aqueous CA solutions are given in Table 4. The VAB values for glycine (except at 298.15 K) and l-alanine in aqueous CA solutions are found to be negative at different temperatures. On the other hand, the VABB values are negative for glycine and positive for l-alanine over the entire temperature range. From Table 4 it is also observed that the magnitude of VAB and VABB values for l-alanine are greater than for glycine in CA, which suggest that l-alanine interacts more strongly with CA. Overall, the higher magnitude of VABB values for l-alanine in CA at the studied temperatures indicates the dominance of triplet interactions. Pair interactions dominate for glycine in the presence of CA at lower temperatures (i.e. 288.15 and 298.15 K) whereas at higher temperatures (i.e. 308.15, 310.15 and 318.15 K) triplet interactions dominate. The reverse trend of VAB and VABB values has been observed for glycine in SA, i.e. VAB dominates at high temperature whereas VABB dominates at low temperature (no specific reason mentioned). The observed behavior of VAB and VABB values for glycine in CA may be attributed to the presence of some cooperativity in the interaction of the alkyl group (hydrocarbon part), i.e. when two hydrocarbon groups come in contact with each other then it is easier for the third group to join the other two [45].
Table 4

Pair and triplet interaction coefficients of glycine/l-alanine in aqueous CA solutions at T = (288.15 to 318.15) K

T(K) \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ 10^{6} \times V_{{{\text{AB}}}} \,({\text{m}}^{3}{ \cdot}{\text{mol}}^{{ - 2}} {\cdot}{\text{kg)}} $$\end{document}106×VAB(m3·mol-2·kg) \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ 10^{6} \times V_{{{\text{ABB}}}} $$\end{document}106×VABB \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ ({\text{m}}^{3} {\cdot}{\text{mol}}^{ - 3} {\cdot}{\text{kg}}^{2} ) $$\end{document}(m3·mol-3·kg2)
Glycine
 288.15− 0.33 (− 12.28)SA− 1.62 (12.69)SA
 298.150.19 (− 11.68)SA− 5.07 (3.82)SA
 308.15− 1.40 (0.64)SA− 1.69 (− 33.95)SA
 310.15− 1.40 (0.91)SA− 0.22 (− 35.34)SA
 318.15− 1.64 (1.22)SA− 0.59 (− 39.19)SA
l-Alanine
 288.15− 1.321.75
 298.15− 5.8213.51
 308.15− 8.3519.42
 310.15− 8.9921.48
 318.15− 9.2521.58

()SA are the and values of glycine in aqueous SA solutions [23]

Pair and triplet interaction coefficients of glycine/l-alanine in aqueous CA solutions at T = (288.15 to 318.15) K ()SA are the and values of glycine in aqueous SA solutions [23] To study the effect of temperature on , the limiting partial molar expansibilities and their second-order derivatives have been calculated by fitting the following equation to the corresponding data:where a, b and c are constants and T is the absolute temperature. The and values of glycine/l-alanine in water are (0.097 m3·mol−1·K−1, − 0.0023 m3·mol−1·K−2) and (0.079 m3·mol−1·K−1, − 0.0019 m3·mol−1·K−2), respectively, which agree well with the literature values [27, 28, 34] and are summarized in Table 5. The values of glycine in aqueous CA solutions decrease with increasing temperature except at  = (0.4 and 0.5) mol·kg−1 whereas the values for l-alanine increase with increasing temperature.
Table 5

Limiting partial molar expansibilities and their second-order derivatives for glycine/l-alanine in water and in aqueous CA solutions at T = (288.15 to 318.15) K

\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ m_{\text{c}} $$\end{document}mc (mol·kg−1)\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ (\partial \phi _{V}^{{\text{o}}} /\partial T)_{p} $$\end{document}(∂ϕVo/∂T)p (m3·mol−1·K−1)\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ (\partial ^{2} \phi _{V}^{{\text{o}}} /\partial T^{2} )_{p} $$\end{document}(∂2ϕVo/∂T2)p (m3·mol−1·K−2)
T(K): 288.15298.15308.15310.15318.15
Glycine
 0.000.096a(0.097)b0.072a(0.072)b(0.071)c0.049a(0.047)b0.044a0.025a(0.022)b− 0.0024a(− 0.0025)b
 0.050.0840.0670.0490.0460.032− 0.0018
 0.100.097(0.125)SA0.071(0.093)SA0.044(0.061)SA0.039(0.054)SA0.018(0.029)SA− 0.0026(− 0.0032)SA
 0.200.0750.0540.0330.0290.012− 0.0021
 0.300.056(0.486)SA0.048(0.416)SA0.041(0.346)SA0.040(0.332)SA0.034(0.276)SA− 0.0007(− 0.0074)SA
 0.400.0410.0490.0570.0580.0650.0008
 0.500.027(− 0.219)SA0.045(− 0.149)SA0.063(− 0.079)SA0.067(− 0.065)SA0.081(− 0.009)SA0.0018(0.0075)SA
l-Alanine
 0.000.0790.060(0.062)d0.0400.0360.021− 0.0019
 0.050.0080.0100.0200.0220.029− 0.0010
 0.100.0040.0100.0170.0180.0240.0007
 0.200.0120.0130.0140.0140.0150.0001
 0.300.0090.0120.0150.0160.0180.0003
 0.400.0100.0130.0150.0150.0170.0002
 0.500.0070.0110.0150.0160.0190.0004

()SA are the and values of glycine in aqueous SA solutions [23]

aPresent work

bReference [27]

cReference [28]

dReference [34]

Limiting partial molar expansibilities and their second-order derivatives for glycine/l-alanine in water and in aqueous CA solutions at T = (288.15 to 318.15) K ()SA are the and values of glycine in aqueous SA solutions [23] aPresent work bReference [27] cReference [28] dReference [34] Hepler [46] used the following thermodynamic relation by which qualitative information regarding hydration of a solute can be evaluated from the thermal expansion: where is the partial molar heat capacity. The sign of and its temperature dependence should provide a distinction between the structure making or breaking ability of solutes in solution. According to Eq. 7, a structure-breaking solute should have negative values whereas positive values suggest that the solute behaves as a structure maker. It can be seen from Table 5 that glycine predominantly acts as a structure breaker whereas l-alanine acts as a structure maker. It is concluded that the structure-breaking and structure-making behavior of glycine and l-alanine in CA may be attributed to the absence of the caging effect [47]. Similar behavior for glycine in SA is observed for the values. Overall, the structure breaking tendency of glycine in the presence of CA is higher than for glycine in SA [23]. This may be attributed due to partial dissociation of CA which tends to destruct the hydrogen bonded structure of water, and then water behaves as a normal (i.e. non associated) liquid. The equilibrium of different water species (i.e., a hydrogen bonded structure associated with a normal liquid) is temperature dependent. Elevation in temperature leads to the expansion of volume with increase in the fraction of non-associated water molecules. In addition, the water structure changes from the combined effects of increased temperature and with increase in the CA concentration [19].

Hydration Number

The hydration number reflects the electrostriction effect of the charge centers of amino acids on nearby water molecules. Millero et al. [48] reported a relationship between the limiting electrostriction contribution to the partial molar volume and hydration number of the non-electrolytes as:where is the limiting molar volume of electrostricted water and is the molar volume of bulk water. For every water molecule taken from the bulk phase to the region near an AA, the values are − 2.9, − 3.3 and − 4.0) × 10−2 m3·mol−1 at T = (288.15, 298.15 and 308.15) K, respectively [48]. The values of glycine/l-alanine in water and in aqueous CA solutions are summarized in Table 6. The values of glycine/l-alanine in water are in good agreement with the literature values and are shown in Figs. 13 and 14 [26–28, 49]. The values of glycine and l-alanine increase with increase in the concentration of CA, which further suggests that water in the immediate vicinity of charged centers of glycine/l-alanine in aqueous CA solutions is highly electrostricted leading to higher values. The decrease in values with increases in temperature may be attributed to the weakening of the electrostriction effect of charged centers, which then leads to stronger interactions between glycine–CA and l-alanine–CA. It also shows that CA exerts a dehydration effect in the present systems.
Table 6

Hydration number of glycine/l-alanine in water and in aqueous CA solutions at T = (288.15, 298.15 and 308.15) K

mc (mol·kg−1) \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$n_{\text{H}}$$\end{document}nH
T(K): 288.15298.15308.15
Glycine
 0.003.27a(3.26b, 3.27c, 3.28d)2.64a(2.58b, 2.60c, 2.61d)2.00a a(2.02b,c, 1.97d)
 0.053.292.642.06
 0.103.34 (3.97)SA2.66 (3.20) SA2.08 (2.55) SA
 0.203.372.782.18
 0.303.41 (5.83) SA2.85 (4.37) SA2.24 (2.38) SA
 0.403.603.042.39
 0.503.62 (6.53) SA3.10 (5.27) SA2.40 (4.32) SA
l-Alanine
 0.004.17a(4.17d)3.45a(3.45b)2.73a(2.68b, 2.72d)
 0.054.213.683.01
 0.104.243.713.03
 0.204.293.723.05
 0.304.323.773.08
 0.404.353.783.10
 0.504.383.833.12

()SA are the values of glycine in aqueous SA solutions [23]

aPresent work

bReference [28]

cReference [27]

dReference [49]

Fig. 13

Comparison plots of hydration number ( of glycine in water at different temperatures, T = (288.15, 298.15 and 308.15) K

Fig. 14

Comparison plots of hydration number ( of l-alanine in water at different temperatures, T = (288.15, 298.15 and 308.15) K

Hydration number of glycine/l-alanine in water and in aqueous CA solutions at T = (288.15, 298.15 and 308.15) K ()SA are the values of glycine in aqueous SA solutions [23] aPresent work bReference [28] cReference [27] dReference [49] Comparison plots of hydration number ( of glycine in water at different temperatures, T = (288.15, 298.15 and 308.15) K Comparison plots of hydration number ( of l-alanine in water at different temperatures, T = (288.15, 298.15 and 308.15) K Lower values for glycine/l-alanine in water than in CA indicate that glycine/l-alanine are more hydrated in the presence of CA. Also, the higher values for glycine in SA than in CA further suggests that SA has a strong dehydrating effect on glycine. This may be due to the presence of hydrophilic groups (i.e., –OH and –COOH in CA) which leads to the formation of hydrogen bonding.

Apparent Specific Volumes and Taste Quality

CA and inorganic citrates are active ingredients in many dosage forms. It is a natural preservative which is used to add an acidic or sour taste to foods and drinks. The taste behavior can be verified on the basis of the apparent specific volumes () which gives a direct measure of the dislocation of water molecule by the solute and reflects its compatibility with water. has been calculated by using the following equation [50]:where is the apparent molar volume and M is the molar mass of glycine/l-alanine and bears a relationship to taste quality in the order salt < ∼ 0.33, sour ∼ 0.33 to ~ 0.52, sweet ∼ 0.52 to ~ 0.71, and bitter ∼ 0.71 to ∼ 0.93 [51]. The values for CA in water, glycine/l-alanine in water, in aqueous CA and glycine in aqueous SA solutions are given in Table S2. It is observed from Table S2 that the values of glycine/l-alanine in water and in aqueous CA solutions (range from 0.55 to 0.69) fall in the sweet taste behavior range (except for l-alanine at  = 0.05 ), which may be due to hydrophobic interactions occurring in these ternary systems. The result obtained from the values also supports data, which further strengthens the view that hydrophobic interactions are dominating in these systems. In the case of SA, the values of glycine tend to show sour-to-sweet taste behavior (ranges from 0.47 to 0.61) with increase in concentration and temperature. The obtained trend in SA may due to the displacement of a large number of water molecules by hydrophobic groups [52].

Conclusions

The negative values obtained for glycine/l-alanine in aqueous CA solutions suggest the dominance of hydrophilic–hydrophobic and hydrophobic–hydrophobic interactions in these systems. Triplet interactions dominate over pair interactions in the case of l-alanine compared to glycine in CA, which indicates that l-alanine interacts more strongly with CA. At low concentrations of CA, glycine/l-alanine act as structure breakers whereas at high concentrations of CA, glycine/l-alanine act as structure makers. Also, it is evident that CA has a dehydration effect on amino acids. The results obtained for values suggest that CA enhances the sweet taste behavior of glycine/l-alanine with rise in temperatures. Comparative studies of glycine in aqueous CA and aqueous SA solutions show that the stronger interactions exist between glycine–CA than for glycine–SA, which is attributed due to the presence of additional hydrophilic groups in CA that leads to the formation of hydrogen bonds. Below is the link to the electronic supplementary material. Supplementary material 1 (DOCX 61 kb)
  6 in total

1.  Some taste molecules and their solution properties.

Authors:  S A Parke; G G Birch; R Dijk
Journal:  Chem Senses       Date:  1999-06       Impact factor: 3.160

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Authors:  Hua Zhao
Journal:  Biophys Chem       Date:  2006-05-09       Impact factor: 2.352

Review 3.  Amino acids: metabolism, functions, and nutrition.

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Journal:  Amino Acids       Date:  2009-03-20       Impact factor: 3.520

Review 4.  Thermodynamic analysis of biomolecules: a volumetric approach.

Authors:  T V Chalikian; K J Breslauer
Journal:  Curr Opin Struct Biol       Date:  1998-10       Impact factor: 6.809

5.  Natural deep eutectic solvents as new potential media for green technology.

Authors:  Yuntao Dai; Jaap van Spronsen; Geert-Jan Witkamp; Robert Verpoorte; Young Hae Choi
Journal:  Anal Chim Acta       Date:  2013-01-09       Impact factor: 6.558

6.  Calculation of the aqueous thermodynamic properties of citric acid cycle intermediates and precursors and the estimation of high temperature and pressure equation of state parameters.

Authors:  Peter Dalla-Betta; Mitchell Schulte
Journal:  Int J Mol Sci       Date:  2009-06-22       Impact factor: 6.208
  6 in total

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