Jie Gong1, Mingrui Zhang2, Yu Wang3, Ruoyu Li4,5,6, Lihua He1, Zhe Wan4,5,6, Fuqiu Li2, Jianzhong Zhang1. 1. 1State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, PR China. 2. 2Department of Dermatology, The Second Hospital of Jilin University, ChangChun, 130041, PR China. 3. 3Key Laboratory of Wildlife Biotechnology, Conservation and Utilization of Zhejiang Province, Zhejiang Normal University, Jinhua, 321004, PR China. 4. 4Department of Dermatology, Peking University First Hospital, Beijing, 100034, PR China. 5. 5Beijing Key Laboratory of Molecular Diagnosis on Dermatoses, Beijing, 100034, PR China. 6. 6Research Center for Medical Mycology, Peking University, Beijing, 100034, PR China.
Abstract
PURPOSE: Sporothrix globosa is the most important agent of sporotrichosis in China. The aim of this study is to investigate the population parameters of S. globosa. METHODOLOGY: In the present study, we developed a set of microsatellite markers that have a cumulative discriminatory power of 1.000. Using these microsatellite loci, 120 strains of S. globosa that had clear sampling information were analysed. RESULTS: Population structure analyses revealed that S. globosa can be separated into three clusters. Analysis of molecular variance (AMOVA) results indicated that genetic variation was more significant among these three clusters than between the two clinical types analysed. In addition, cluster II might have the widest range of distribution and contain higher genetic diversity than the other clusters. CONCLUSIONS: Our work is the first to develop a suite of highly discriminatory microsatellite markers and reveal the population parameters of S. globosa, and our results suggest that different lineages can coexist in two different clinical types. In addition, it was hypothesised that lineages with higher genetic diversity might have a wider distribution range.
PURPOSE:Sporothrix globosa is the most important agent of sporotrichosis in China. The aim of this study is to investigate the population parameters of S. globosa. METHODOLOGY: In the present study, we developed a set of microsatellite markers that have a cumulative discriminatory power of 1.000. Using these microsatellite loci, 120 strains of S. globosa that had clear sampling information were analysed. RESULTS: Population structure analyses revealed that S. globosa can be separated into three clusters. Analysis of molecular variance (AMOVA) results indicated that genetic variation was more significant among these three clusters than between the two clinical types analysed. In addition, cluster II might have the widest range of distribution and contain higher genetic diversity than the other clusters. CONCLUSIONS: Our work is the first to develop a suite of highly discriminatory microsatellite markers and reveal the population parameters of S. globosa, and our results suggest that different lineages can coexist in two different clinical types. In addition, it was hypothesised that lineages with higher genetic diversity might have a wider distribution range.
Entities:
Keywords:
Sporothrix globosa; genetic diversity; microsatellites; population structure; sporotrichosis
Authors: J A de Carvalho; M A Beale; F Hagen; M C Fisher; R Kano; A Bonifaz; C Toriello; R Negroni; R S de M Rego; I D F Gremião; S A Pereira; Z P de Camargo; A M Rodrigues Journal: Stud Mycol Date: 2021-12-17 Impact factor: 16.097