Zerihun A Demissie1,2, Mike Tarnowycz3, Ayelign M Adal3, Lukman S Sarker3, Soheil S Mahmoud4. 1. Department of Biology, University of British Columbia, Kelowna, BC, V1V 1V7, Canada. Zerihun.demissie@nrc.ca. 2. National Research Council of Canada, Ottawa, ON, K1A 0R6, Canada. Zerihun.demissie@nrc.ca. 3. Department of Biology, University of British Columbia, Kelowna, BC, V1V 1V7, Canada. 4. Department of Biology, University of British Columbia, Kelowna, BC, V1V 1V7, Canada. soheil.mahmoud@ubc.ca.
Abstract
MAIN CONCLUSION: Functional expression of a multidrug resistance-type ABC transporter from Lavandulaangustifolia improved yeast resistance to geraniol, a monoterpene constituent of lavender essential oil. Plant ATP-binding cassette (ABC) transporters are a large family of membrane proteins involved in active and selective transport of structurally diverse compounds. In this study, we functionally evaluated LaABCB1, a multidrug resistance (MDR)-type ABC transporter strongly expressed in the secretory cells of lavender glandular trichomes, where monoterpene essential oil constituents are synthesized and secreted. We used LaABCB1 to complement a yeast knockout mutant in which 16 ABC transporters were deleted. Expression of LaABCB1 enhanced tolerance of yeast mutants to geraniol, a key constituent of essential oils in lavenders and numerous other plants. Our findings suggest a role for the MDR-type ABC transporters in the toxicity tolerance of at least certain essential oil constituents in lavender oil glands.
MAIN CONCLUSION: Functional expression of a multidrug resistance-type ABC transporter from Lavandulaangustifolia improved yeast resistance to geraniol, a monoterpene constituent of lavender essential oil. Plant ATP-binding cassette (ABC) transporters are a large family of membrane proteins involved in active and selective transport of structurally diverse compounds. In this study, we functionally evaluated LaABCB1, a multidrug resistance (MDR)-type ABC transporter strongly expressed in the secretory cells of lavender glandular trichomes, where monoterpene essential oil constituents are synthesized and secreted. We used LaABCB1 to complement a yeast knockout mutant in which 16 ABC transporters were deleted. Expression of LaABCB1 enhanced tolerance of yeast mutants to geraniol, a key constituent of essential oils in lavenders and numerous other plants. Our findings suggest a role for the MDR-type ABC transporters in the toxicity tolerance of at least certain essential oil constituents in lavender oil glands.
Entities:
Keywords:
ATP-binding cassette; Lavandula; Monoterpene trafficking; Multidrug resistance; Transmembrane protein
Authors: Eloisa Vendemiatti; Rodrigo Therezan; Mateus H Vicente; Maísa de Siqueira Pinto; Nick Bergau; Lina Yang; Walter Fernando Bernardi; Severino M de Alencar; Agustin Zsögön; Alain Tissier; Vagner A Benedito; Lázaro E P Peres Journal: Plants (Basel) Date: 2022-05-14