Literature DB >> 17401334

High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method.

R Daniel Gietz1, Robert H Schiestl.   

Abstract

Here we describe a high-efficiency version of the lithium acetate/single-stranded carrier DNA/PEG method of transformation of Saccharomyces cerevisiae. This method currently gives the highest efficiency and yield of transformants, although a faster protocol is available for small number of transformations. The procedure takes up to 1.5 h, depending on the length of heat shock, once the yeast culture has been grown. This method is useful for most transformation requirements.

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Year:  2007        PMID： 17401334     DOI： 10.1038/nprot.2007.13

Source DB:  PubMed          Journal:  Nat Protoc        ISSN： 1750-2799            Impact factor:   13.491


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