| Literature DB >> 30535700 |
Noha A S Al-Otaibi1,2, Nigel K H Slater1, Hassan Rahmoune3.
Abstract
Glycerol and trehalose are widely used protective agents in the cryopreservation of red blood cells (RBCs). This chapter presents a protocol for use of these agents as cryoprotectants of RBCs, with post-thaw assessment of cell survival and cellular oxidative-reductive status. The main aim is to provide a framework for further studies aimed at improving RBC survival and function and to supply improved biomaterials for studies on RBC biochemistry, major operations, as well as those for urgent use in emergency room situations.Entities:
Keywords: Cell proliferation; Cell survival; Cryopreservation; Glycerol; Oxidative–reductive status; Red blood cell; Trehalose
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Year: 2019 PMID： 30535700 DOI： 10.1007/978-1-4939-8994-2_22
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745