Draft Genome Sequences of Lawsonia intracellularis Swine Strains Causing Proliferative Enteropathy in Japan.

The draft genome sequences of three strains of Lawsonia intracellularis, an obligate intracellular animal pathogen responsible for causing proliferative enteropathy, obtained from swine in different prefectures in Japan revealed the absence of a genomic island previously reported to be linked to host adaptation and to high genomic diversity, despite geographical proximity.

ANNOUNCEMENT

Lawsonia intracellularis is a Gram-negative obligate intracellular bacterium that causes proliferative enteropathy (PE) in a wide range of animal species (1). Porcine PE, which is characterized by thickening of intestinal mucosa due to hyperplasia of crypt epithelia/enterocytes with inflammation in the jejunum, ileum, and colon, mainly affects growing/finishing pigs and results in substantial economic losses in the pig industry (1). So far, only three strains have been sequenced, two swine strains, PHE/MN1-00 and N343 (2), and an equine strain, E40504 (3). In this article, we report the draft genome sequences of three swine strains.

Strains Fu/JPN, Ni/JPN, and Ib2/JPN were all isolated from the thickened mucous membrane of the ileums of swine macroscopically diagnosed with PE at slaughterhouses in three different prefectures in Japan. The intestines were cut into approximately 15-cm lengths and opened longitudinally. The lumen was washed, disinfected with a 10% povidone-iodine solution, and rinsed with phosphate-buffered saline (PBS). The mucous membrane lesions were isolated, suspended in PBS, and incubated at 37°C for 30 min in PBS containing 0.05% trypsin and 0.02% EDTA. After incubation, cell debris was removed by centrifugation at 1,000 rpm for 8 min, and the supernatant was filtered through a filter paper and then centrifuged at 15,000 rpm for 15 min. The pellets were subjected to DNA extraction using the QIAamp DNA minikit (Qiagen, CA) and were confirmed to include L. intracellularis genomic DNA by PCR with the L. intracellularis-specific primers LI-No.6-F (5ʹ-TCTCAGTGAGATGGGATCATGGATGGATAG-3ʹ) and LI-No.6-R (5ʹ-GAACTTGGCTCATTAACTCGCTTACTCCAT-3ʹ), both of which were designed based on the reference genome sequence of strain PHE/MN1-00. DNA libraries were prepared using the TruSeq DNA PCR-free library prep kit (Illumina, CA) according to the manufacturer’s instructions. Sequencing was performed using an Illumina HiSeq X Ten sequencing platform (150-bp paired-end reads). Following trimming of low-quality reads with ConDeTri (4), mapping of the Illumina reads to the reference genome sequence and the single-nucleotide polymorphism (SNP) calling was performed as previously described (5).

The numbers of short reads of the Fu/JPN, Ni/JPN, and Ib2/JPN strains were 3,033,172 bp, 3,282,666 bp, and 13,115,606 bp, respectively, all of which were mapped at high sequence identity (>99.0%), resulting in 286-, 301-, and 1,172-fold sequence depths, respectively. The proportions of the short reads to the total sequence reads obtained with the pig samples were 3.70% (Fu/JPN), 3.55% (Ni/JPN), and 15.67% (Ib2/JPN). The mapped reads were extracted using SAMtools version 0.1.19 (6) and assembled using Platanus version 1.2.4 (7). In the three strains, the final assembly comprised 6 (Fu/JPN and Ni/JPN) or 7 contigs (Ib2/JPN) (>1,000 bp), with a total genome size of 1.69 Mb, a GC content of 32.9%, and an N50 value of 700 kb, as evaluated by QUAST (8). Compared to the reference genome sequence of strain PHE/MN1-00, the U.S. strain N343 has 24 SNPs, whereas strains Fu/JPN, Ni/JPN, and Ib2/JPN have 99, 456, and 552 SNPs, respectively, indicating the genetic diversity of the Japanese strains. The 18-kb genomic island previously reported to be linked to the host adaptation (9) was missing from the genomic assembly of these Japanese strains.

Data availability.

The draft genome sequences have been deposited at DDBJ/EMBL/GenBank under the accession numbers QNHO00000000 (Fu/JPN), QNHN00000000 (Ni/JPN), and QNHM00000000 (Ib2/JPN). Raw data are available in the NCBI Sequence Read Archive (SRA) database with accession numbers DRX133711 (Fu/JPN), DRX133712 (Ni/JPN), and DRX133713 (Ib2/JPN).