Hércules Bezerra Dias1, Maria Inês Basso Bernardi2, Taís Maria Bauab3, Antônio Carlos Hernandes2, Alessandra Nara de Souza Rastelli4. 1. Department of Restorative Dentistry, Araraquara School of Dentistry, University of São Paulo State - UNESP, Araraquara, SP 14801-903, Brazil. 2. Department of Physics and Materials Science, Physics Institute of São Carlos - IFSC, University of São Paulo - USP, São Carlos, SP 13566-590, Brazil. 3. Department of Biological Sciences, Pharmaceutical Sciences School, University of São Paulo State - UNESP, Araraquara, SP 14801-902, Brazil. 4. Department of Restorative Dentistry, Araraquara School of Dentistry, University of São Paulo State - UNESP, Araraquara, SP 14801-903, Brazil. Electronic address: alrastelli@foar.unesp.br.
Abstract
OBJECTIVE: The aim of this study was to evaluate the antibacterial activity of a composite resin modified by TiO2 and TiO2/Ag nanoparticles and their influence over different properties. METHODS: TiO2 and TiO2/Ag NPs were synthesized by polymeric precursor and microwave-assisted hydrothermal methods and then, characterized by different techniques. Direct contact test was performed using Filtek™ Z350XT blended with 0.5; 1 and 2% (wt.) of NPs against Streptococcus mutans to determine the best concentration to the other tests. After that, the modified composite resin was tested against S. mutans 7-day biofilm (CFU/mL). Also, compressive and diametral tensile strength (n=40), degree of conversion (n=25) and surface roughness (n=50) was performed. The data were analyzed by ANOVA and Tukey's test for multiple comparison at 5% significance level. RESULTS: The direct contact test demonstrates that by increasing the nanoparticle content, the bacterial growth is significantly reduceed (p<0.05). The inclusion of 2% of TiO2/Ag NPs significantly decreased (p<0.05) the biofilm accumulation of S. mutans on the composite resin surface compared to the control Group. The TiO2 NPs treated with an organosilane increased compressive strength of composite resin (p<0.05). Degree of conversion remained unchanged (p>0.05) and the surface roughness increased with the NPs (p<0.05), except for the TiO2 by polymeric precursor Group (p>0.05). SIGNIFICANCE: The development of an antibacterial dental restorative material that hinder S. mutans biofilm without sacrificing the mechanical and physical properties is desirable in dental material science.
OBJECTIVE: The aim of this study was to evaluate the antibacterial activity of a composite resin modified by TiO2 and TiO2/Ag nanoparticles and their influence over different properties. METHODS:TiO2 and TiO2/Ag NPs were synthesized by polymeric precursor and microwave-assisted hydrothermal methods and then, characterized by different techniques. Direct contact test was performed using Filtek™ Z350XT blended with 0.5; 1 and 2% (wt.) of NPs against Streptococcus mutans to determine the best concentration to the other tests. After that, the modified composite resin was tested against S. mutans 7-day biofilm (CFU/mL). Also, compressive and diametral tensile strength (n=40), degree of conversion (n=25) and surface roughness (n=50) was performed. The data were analyzed by ANOVA and Tukey's test for multiple comparison at 5% significance level. RESULTS: The direct contact test demonstrates that by increasing the nanoparticle content, the bacterial growth is significantly reduceed (p<0.05). The inclusion of 2% of TiO2/Ag NPs significantly decreased (p<0.05) the biofilm accumulation of S. mutans on the composite resin surface compared to the control Group. The TiO2 NPs treated with an organosilane increased compressive strength of composite resin (p<0.05). Degree of conversion remained unchanged (p>0.05) and the surface roughness increased with the NPs (p<0.05), except for the TiO2 by polymeric precursor Group (p>0.05). SIGNIFICANCE: The development of an antibacterial dental restorative material that hinder S. mutans biofilm without sacrificing the mechanical and physical properties is desirable in dental material science.
Authors: José Marcos Dos Santos Oliveira; Théo Fortes Silveira Cavalcanti; Ingrid Ferreira Leite; Dávida Maria Ribeiro Cardoso Dos Santos; Isabel Cristina Celerino de Moraes Porto; Fernanda Lima Torres de Aquino; Artur Falqueto Sonsin; Renata Matos Lamenha Lins; Rafael Pino Vitti; Johnnatan Duarte de Freitas; Emiliano de Oliveira Barreto; Samuel Teixeira de Souza; Regianne Umeko Kamiya; Ticiano Gomes do Nascimento; Josealdo Tonholo Journal: Front Pharmacol Date: 2021-11-29 Impact factor: 5.810