Literature DB >> 30520916

Posttranscriptional spin labeling of RNA by tetrazine-based cycloaddition.

Christof Domnick1, Gregor Hagelueken, Frank Eggert, Olav Schiemann, Stephanie Kath-Schorr.   

Abstract

The site-specific introduction of spin labels into RNA for distance measurements by EPR gives insight into its solution structure. We here present a method for spin labeling of in vitro transcribed RNA. Distance distributions between two nitroxide spin labels are determined by PELDOR in a self-complementary RNA duplex.

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Year:  2019        PMID： 30520916     DOI： 10.1039/c8ob02597e

Source DB:  PubMed          Journal:  Org Biomol Chem        ISSN： 1477-0520            Impact factor:   3.876


  7 in total

1.  Preparation of Site-Specifically Spin-Labeled RNA by in Vitro Transcription Using an Expanded Genetic Alphabet.

Authors:  Lisa Bornewasser; Stephanie Kath-Schorr
Journal:  Methods Mol Biol       Date:  2022

Review 2.  Paramagnetic Chemical Probes for Studying Biological Macromolecules.

Authors:  Qing Miao; Christoph Nitsche; Henry Orton; Mark Overhand; Gottfried Otting; Marcellus Ubbink
Journal:  Chem Rev       Date:  2022-01-27       Impact factor: 72.087

3.  Stronger together for in-cell translation: natural and unnatural base modified mRNA.

Authors:  Lisa Bornewasser; Christof Domnick; Stephanie Kath-Schorr
Journal:  Chem Sci       Date:  2022-03-03       Impact factor: 9.969

4.  EPR Distance Measurements on Long Non-coding RNAs Empowered by Genetic Alphabet Expansion Transcription.

Authors:  Christof Domnick; Frank Eggert; Christine Wuebben; Lisa Bornewasser; Gregor Hagelueken; Olav Schiemann; Stephanie Kath-Schorr
Journal:  Angew Chem Int Ed Engl       Date:  2020-03-13       Impact factor: 15.336

5.  Protein Spin Labeling with a Photocaged Nitroxide Using Diels-Alder Chemistry.

Authors:  Anandi Kugele; Bjarne Silkenath; Jakob Langer; Valentin Wittmann; Malte Drescher
Journal:  Chembiochem       Date:  2019-08-14       Impact factor: 3.164

6.  Genetic Code Expansion Facilitates Position-Selective Labeling of RNA for Biophysical Studies.

Authors:  Andreas Hegelein; Diana Müller; Sylvester Größl; Michael Göbel; Martin Hengesbach; Harald Schwalbe
Journal:  Chemistry       Date:  2020-01-21       Impact factor: 5.236

Review 7.  Strategies for Covalent Labeling of Long RNAs.

Authors:  Hannah Depmeier; Eva Hoffmann; Lisa Bornewasser; Stephanie Kath-Schorr
Journal:  Chembiochem       Date:  2021-06-17       Impact factor: 3.164
  7 in total

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