| Literature DB >> 30519855 |
Nan Cheng1,2, Yongkun Zhao1,3, Qiuxue Han1,4, Weijiao Zhang1, Ji Xi5, Yongle Yu5, Hualei Wang1,3, Guohua Li1,6, Yuwei Gao1,3, Songtao Yang7,8, Weiquan Liu9,10, Xianzhu Xia11,12.
Abstract
Feline panleukopenia virus (FPV) infects cats and can be fatal to kittens. There is evidence that canine parvovirus originated from FPV, which makes FPV important in studies of the family Parvoviridae. In the present study, the entire genome of FPV strain HH-1/86 was converted into a full-length infectious clone (pFPV). The FPV strain HH-1/86 has a 5123-nt single stranded DNA genome with a Y-shaped inverted 3' terminal repeat (ITR) and a U-shaped inverted 5' ITR. Feline kidney cells (F81) were transfected with the pFPV clone which contained a genetic marker, and a rescued virus was obtained (rFPV). The rFPV was identified by its cytopathic effects, indirect immunofluorescence, growth curve analysis, western blot assay and hemagglutination, and was indistinguishable from the parent virus. The FPV infectious clone will facilitate the study of pathogenicity and viral replication of FPV and the inter-species transmission of parvoviruses.Entities:
Keywords: Biological properties; Feline panleukopenia virus; Infectious clone; Rescue virus
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Year: 2018 PMID： 30519855 DOI： 10.1007/s11262-018-1621-9
Source DB: PubMed Journal: Virus Genes ISSN： 0920-8569 Impact factor: 2.332