Mahdieh Nazari-Robati1,2, Mahboobe Akbari1, Mohammad Khaksari3, Moghaddameh Mirzaee4. 1. a Department of Clinical Biochemistry, Afzalipour School of Medicine , Kerman University of Medical Sciences , Kerman , Iran. 2. b Neuroscience Research Center , Institute of Neuropharmacology, Kerman University of Medical Sciences , Kerman , Iran. 3. c Endocrinology and Metabolism Research Center , Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences , Kerman , Iran. 4. d Department of Biostatistics and Epidemiology , School of Public Health, Kerman University of Medical Sciences , Kerman , Iran.
Abstract
OBJECTIVE: Inflammation and oxidative stress are implicated in pathogenesis of spinal cord injury (SCI). Trehalose, a nonreducing disaccharide, exhibits anti-inflammatory and antioxidant effects. The present study investigated the therapeutic efficacy of trehalose in the SCI model. DESIGN AND SETTING: An experimental study was designed using 120 male Wistar rats which were randomly divided into three groups including SCI, SCI + phosphate buffer saline (vehicle) and SCI + trehalose. All rats were subjected to SCI. Immediately after SCI, vehicle and trehalose groups received intrathecal injection of buffer and trehalose, respectively. OUTCOME MEASURES: The level of tissue TNFα, IL-1β, nitric oxide, malondialdehyde, myeloperoxidase, glial fibrillary acidic protein (GFAP) as well as hindlimb function were assessed at 4 hours, 1, 3 and 7 days post-SCI. RESULTS: Data indicated an early significant decrease in inflammatory and oxidative responses following SCI in trehalose treated group. Moreover, trehalose reduced GFAP expression as soon as 1-day post-trauma. Furthermore, trehalose treatment increased the score of hindlimb function. CONCLUSION: Our results indicated that treatment with trehalose reduces the development of secondary injury associated with SCI. This effect likely underlies improved neurological function.
OBJECTIVE:Inflammation and oxidative stress are implicated in pathogenesis of spinal cord injury (SCI). Trehalose, a nonreducing disaccharide, exhibits anti-inflammatory and antioxidant effects. The present study investigated the therapeutic efficacy of trehalose in the SCI model. DESIGN AND SETTING: An experimental study was designed using 120 male Wistar rats which were randomly divided into three groups including SCI, SCI + phosphate buffer saline (vehicle) and SCI + trehalose. All rats were subjected to SCI. Immediately after SCI, vehicle and trehalose groups received intrathecal injection of buffer and trehalose, respectively. OUTCOME MEASURES: The level of tissue TNFα, IL-1β, nitric oxide, malondialdehyde, myeloperoxidase, glial fibrillary acidic protein (GFAP) as well as hindlimb function were assessed at 4 hours, 1, 3 and 7 days post-SCI. RESULTS: Data indicated an early significant decrease in inflammatory and oxidative responses following SCI in trehalose treated group. Moreover, trehalose reduced GFAP expression as soon as 1-day post-trauma. Furthermore, trehalose treatment increased the score of hindlimb function. CONCLUSION: Our results indicated that treatment with trehalose reduces the development of secondary injury associated with SCI. This effect likely underlies improved neurological function.
Authors: H B Demopoulos; E S Flamm; M L Seligman; D D Pietronigro; J Tomasula; V DeCrescito Journal: Can J Physiol Pharmacol Date: 1982-11 Impact factor: 2.273
Authors: Bentolhoda Shafiei; Mohammad Shabani; Ali Afgar; Mohammad Amin Rajizadeh; Mahdieh Nazari-Robati Journal: Neurochem Res Date: 2022-07-29 Impact factor: 4.414