Anna Mäkinen1, Ali Nawaz2, Antti Mäkitie3, Jukka H Meurman4. 1. PhD Student, Department of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, Helsinki, Finland. Electronic address: anna.i.makinen@helsinki.fi. 2. PhD Student, Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland. 3. Professor, Department of Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital, Helsinki, Finland; and Division of Ear, Nose and Throat Diseases, Department of Clinical Sciences, Intervention and Technology, Karolinska Institutet and Karolinska Hospital, Stockholm, Sweden. 4. Professor, Department of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Abstract
PURPOSE: This study examined the prevalence of Candida species in the saliva of oral squamous cell carcinoma patients and its effect on the mortality rate. MATERIALS AND METHODS: One hundred patients with oral cavity squamous cell carcinoma undergoing protocol treatment at Helsinki University Hospital were recruited into the study from March 2011 through 2014. For comparison, 75 age-matched controls with no current or previously treated oral cancer were recruited. Paraffin-stimulated whole saliva samples were collected and cultivated on CHROMagar Candida medium (CHROMagar, Paris, France) to establish possible Candida growth. The API ID 32C yeast identification kit (bioMérieux, Lyon, France) and Bichro-Dubli Fumouze latex agglutination test (Fumouze Diagnostics, Levallois-Perret, France) were used for further identification of different Candida species. Patients' medical records were studied for information on their health habits and general health status, as well as tumor-related data. The patients' status regarding being alive and cancer free was checked at a follow-up point in December 2017. Descriptive statistics and cross tabulation were carried out, and the P value was set at .05. RESULTS: Candida species were detected in 74% of the oral cancer patients' samples, with C. albicans being the most common species (84%). Other species identified were C. dubliniensis (8%), C. tropicalis (4%), C. glabrata (3%), C. parapsilosis (3%), C. sake (3%), C. krusei (1%), and C. guilliermondii (1%). After the follow-up period, 63% of the patients were alive and 86% of them were cancer free. Harboring Candida species in the saliva was not associated with any increase in the mortality rate. CONCLUSIONS: C. albicans was common in the oral cavity of the oral squamous cell carcinoma patients. However, in this patient population, we did not observe a statistically significant effect of the yeast on the mortality rate.
PURPOSE: This study examined the prevalence of Candida species in the saliva of oral squamous cell carcinomapatients and its effect on the mortality rate. MATERIALS AND METHODS: One hundred patients with oral cavity squamous cell carcinoma undergoing protocol treatment at Helsinki University Hospital were recruited into the study from March 2011 through 2014. For comparison, 75 age-matched controls with no current or previously treated oral cancer were recruited. Paraffin-stimulated whole saliva samples were collected and cultivated on CHROMagar Candida medium (CHROMagar, Paris, France) to establish possible Candida growth. The API ID 32C yeast identification kit (bioMérieux, Lyon, France) and Bichro-Dubli Fumouze latex agglutination test (Fumouze Diagnostics, Levallois-Perret, France) were used for further identification of different Candida species. Patients' medical records were studied for information on their health habits and general health status, as well as tumor-related data. The patients' status regarding being alive and cancer free was checked at a follow-up point in December 2017. Descriptive statistics and cross tabulation were carried out, and the P value was set at .05. RESULTS:Candida species were detected in 74% of the oral cancerpatients' samples, with C. albicans being the most common species (84%). Other species identified were C. dubliniensis (8%), C. tropicalis (4%), C. glabrata (3%), C. parapsilosis (3%), C. sake (3%), C. krusei (1%), and C. guilliermondii (1%). After the follow-up period, 63% of the patients were alive and 86% of them were cancer free. Harboring Candida species in the saliva was not associated with any increase in the mortality rate. CONCLUSIONS:C. albicans was common in the oral cavity of the oral squamous cell carcinomapatients. However, in this patient population, we did not observe a statistically significant effect of the yeast on the mortality rate.
Authors: Ahmed Gamal; Mohammed Elshaer; Mayyadah Alabdely; Ahmed Kadry; Thomas S McCormick; Mahmoud Ghannoum Journal: Cancers (Basel) Date: 2022-06-10 Impact factor: 6.575
Authors: Máté Vadovics; Jemima Ho; Nóra Igaz; Róbert Alföldi; Dávid Rakk; Éva Veres; Balázs Szücs; Márton Horváth; Renáta Tóth; Attila Szücs; Andrea Csibi; Péter Horváth; László Tiszlavicz; Csaba Vágvölgyi; Joshua D Nosanchuk; András Szekeres; Mónika Kiricsi; Rhonda Henley-Smith; David L Moyes; Selvam Thavaraj; Rhys Brown; László G Puskás; Julian R Naglik; Attila Gácser Journal: mBio Date: 2022-01-04 Impact factor: 7.867