Haotian Wu1, Alexandra M Huffman1, Brian W Whitcomb2, Srinihaari Josyula1, Suzanne Labrie3, Ellen Tougias3, Tayyab Rahil3, Cynthia K Sites3, Jonathan Richard Pilsner4. 1. Department of Environmental Health Sciences, School of Public Health and Health Sciences, University of Massachusetts, 173A Goessmann, 686 North Pleasant Street, Amherst MA 01003, USA. 2. Department of Biostatistics and Epidemiology, School of Public Health and Health Sciences, University of Massachusetts, 715 North Pleasant Street, Amherst MA 01003, USA. 3. Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Baystate Medical Center, 759 Chestnut Street, Springfield MA 01199, USA. 4. Department of Environmental Health Sciences, School of Public Health and Health Sciences, University of Massachusetts, 173A Goessmann, 686 North Pleasant Street, Amherst MA 01003, USA.. Electronic address: rpilsner@umass.edu.
Abstract
RESEARCH QUESTION: To examine associations between sperm mitochondrial DNA copy number (mtDNAcn), sperm mitochondrial DNA deletions (mtDNAdel), semen parameters and clinical infertility in an IVF setting. DESIGN: A total of 125 sperm samples were collected from men undergoing assisted reproductive procedures in an IVF clinic in Western Massachusetts, USA. Sperm mtDNAcn and mtDNAdel were measured by probe-based quantitative polymerase chain reaction. Semen parameters, clinical diagnoses of infertility, and infertility based on consecutive semen parameters, were fitted with mtDNAcn and mtDNAdel in linear models. The utility of sperm mtDNAcn and mtDNAdel to predict infertility was assessed by receiver operating characteristic curves. RESULTS: Adjusting for relevant covariates, both sperm mtDNAcn and mtDNAdel were associated with lower sperm concentration, count, motility and morphology (P ≤ 0.03). Sperm mtDNAcn and mtDNAdel were also associated with increased risks of clinical infertility based on current and consecutive semen samples. Sperm mtDNAcn had high predictive accuracy for consecutive diagnoses of clinical infertility (C-statistic: 0.91), whereas sperm mtDNAdel had moderate predictive accuracy (C-statistic: 0.75). CONCLUSIONS: Sperm mtDNAcn is a measure of consecutive abnormal semen parameters and has promise as a diagnostic test.
RESEARCH QUESTION: To examine associations between sperm mitochondrial DNA copy number (mtDNAcn), sperm mitochondrial DNA deletions (mtDNAdel), semen parameters and clinical infertility in an IVF setting. DESIGN: A total of 125 sperm samples were collected from men undergoing assisted reproductive procedures in an IVF clinic in Western Massachusetts, USA. Sperm mtDNAcn and mtDNAdel were measured by probe-based quantitative polymerase chain reaction. Semen parameters, clinical diagnoses of infertility, and infertility based on consecutive semen parameters, were fitted with mtDNAcn and mtDNAdel in linear models. The utility of sperm mtDNAcn and mtDNAdel to predict infertility was assessed by receiver operating characteristic curves. RESULTS: Adjusting for relevant covariates, both sperm mtDNAcn and mtDNAdel were associated with lower sperm concentration, count, motility and morphology (P ≤ 0.03). Sperm mtDNAcn and mtDNAdel were also associated with increased risks of clinical infertility based on current and consecutive semen samples. Sperm mtDNAcn had high predictive accuracy for consecutive diagnoses of clinical infertility (C-statistic: 0.91), whereas sperm mtDNAdel had moderate predictive accuracy (C-statistic: 0.75). CONCLUSIONS: Sperm mtDNAcn is a measure of consecutive abnormal semen parameters and has promise as a diagnostic test.
Authors: E Ruiz-Pesini; A C Lapeña; C Díez-Sánchez; A Pérez-Martos; J Montoya; E Alvarez; M Díaz; A Urriés; L Montoro; M J López-Pérez; J A Enríquez Journal: Am J Hum Genet Date: 2000-08-09 Impact factor: 11.025
Authors: Allyson J Rosati; Brian W Whitcomb; Nicole Brandon; Germaine M Buck Louis; Sunni L Mumford; Enrique F Schisterman; J Richard Pilsner Journal: Hum Reprod Date: 2020-11-01 Impact factor: 6.918