| Literature DB >> 3048354 |
L Ottestad1, K M Tveit, H K Høifødt, J M Nesland, S Vaage, J Høie, E Lund, A Pihl.
Abstract
A total of 237 breast carcinomas have been studied with the Courtenay-Mills (C-M) soft agar method. Cell yields and plating efficiencies (PE) were recorded after various enzyme treatments. The highest cell yields and PEs were obtained with the combination of collagenase 0.5%, hyaluronidase 1000 IE ml-1 and DNase 0.1% and an incubation time of 2 h. Eighty percent of the specimens gave greater than 10 colonies, and 60% formed greater than 30 colonies permitting chemosensitivity studies. The C-M method gave significantly higher PEs than the Hamburger-Salmon (H-S) method. Hormone supplements (insulin, oestradiol, progesterone, hydrocortisone) and also reduced agar concentrations (less than 0.3%) gave marginal stimulation of colony formation. In chemosensitivity studies involving doxorubicin, vincristine and 4-OOH-cyclophosphamide, the C-M method gave dose-response relationships without plateaus.Entities:
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Year: 1988 PMID： 3048354 PMCID： PMC2246502 DOI： 10.1038/bjc.1988.151
Source DB: PubMed Journal: Br J Cancer ISSN： 0007-0920 Impact factor: 7.640