Yao Dong1, Wei Wu2. 1. Department of stomatology, NingBo No.2 Hospital, No.41 Xibei Street, Haishu district, Ningbo City, 315010, Zhejiang Province, People's Republic of China. gvbiln4@163.com. 2. Department of stomatology, NingBo No.2 Hospital, No.41 Xibei Street, Haishu district, Ningbo City, 315010, Zhejiang Province, People's Republic of China.
Abstract
PURPOSE: LncRNA CASC2 plays a role as tumor suppressor gene in different types of human malignancies, while its involvement in oral squamous cell carcinoma (OSCC) is unknown. The present study aimed to investigate the involvement of lncRNA CASC2 in OSCC. METHODS: In this study, the expression of lncRNA CASC2 in tumor tissues, adjacent healthy tissues, and plasma of 122 OSCC patients as well as in plasma of 52 healthy controls was detected by RT-qPCR. Diagnostic value of lncRNA CASC2 for OSCC was evaluated by ROC curve analysis. Patients were followed up for 5 years to record recurrence. LncRNA CASC2 expression vectors were transfected into cells of human OSCC cell lines, and the effects on cancer cell proliferation and miRNA-21 expression were analyzed by CCK-8 assay and RT-qPCR, respectively. RESULTS: We found that CASC2 was significantly downregulated in OSCC patients than in healthy controls. Downregulation of CASC2 distinguished OSCC patients from healthy controls. Local recurrence was observed in 26 out of 122 patients and no distant recurrence was observed during follow-up. Compared with pretreatment levels, plasma levels of CASC2 were significantly increased in patients with local recurrence than in patients without recurrence. Transfection of CASC2 expression vectors led to significantly inhibited tumor cell proliferation and reduced miRNA-21 expression levels. CONCLUSIONS: We, therefore, conclude that downregulation of lncRNA CASC2 may participate in the postoperative local recurrence of early OSCC through miRNA-21.
PURPOSE: LncRNA CASC2 plays a role as tumor suppressor gene in different types of humanmalignancies, while its involvement in oral squamous cell carcinoma (OSCC) is unknown. The present study aimed to investigate the involvement of lncRNA CASC2 in OSCC. METHODS: In this study, the expression of lncRNA CASC2 in tumor tissues, adjacent healthy tissues, and plasma of 122 OSCC patients as well as in plasma of 52 healthy controls was detected by RT-qPCR. Diagnostic value of lncRNA CASC2 for OSCC was evaluated by ROC curve analysis. Patients were followed up for 5 years to record recurrence. LncRNA CASC2expression vectors were transfected into cells of human OSCC cell lines, and the effects on cancer cell proliferation and miRNA-21expression were analyzed by CCK-8 assay and RT-qPCR, respectively. RESULTS: We found that CASC2 was significantly downregulated in OSCC patients than in healthy controls. Downregulation of CASC2 distinguished OSCC patients from healthy controls. Local recurrence was observed in 26 out of 122 patients and no distant recurrence was observed during follow-up. Compared with pretreatment levels, plasma levels of CASC2 were significantly increased in patients with local recurrence than in patients without recurrence. Transfection of CASC2expression vectors led to significantly inhibited tumor cell proliferation and reduced miRNA-21expression levels. CONCLUSIONS: We, therefore, conclude that downregulation of lncRNA CASC2 may participate in the postoperative local recurrence of early OSCC through miRNA-21.
Entities:
Keywords:
LncRNA CASC2; Local recurrence, miRNA; Oral squamous cell carcinoma