Literature DB >> 3040892

Inhibition of restriction in Streptomyces clavuligerus by heat treatment.

C R Bailey, D J Winstanley.   

Abstract

Inefficient transformation of Streptomyces clavuligerus protoplasts by DNA from the plasmid pIJ702, isolated from S. lividans, was attributed to restriction in view of the observation that efficient transformation was observed using modified pIJ702 (isolated from S. clavuligerus). The restriction system could be partially inhibited by treating protoplasts at 45 degrees C prior to transformation. This treatment increased the transformation frequencies of pIJ702 DNA by 100-fold and was used to introduce other plasmids into S. clavuligerus.

Entities:  

Mesh：

Substances：

Year:  1986        PMID： 3040892     DOI： 10.1099/00221287-132-10-2945

Source DB:  PubMed          Journal:  J Gen Microbiol        ISSN： 0022-1287


  17 in total

1.  Early cephamycin biosynthetic genes are expressed from a polycistronic transcript in Streptomyces clavuligerus.

Authors:  D C Alexander; M J Brumlik; L Lee; S E Jensen
Journal:  J Bacteriol       Date:  2000-01       Impact factor: 3.490

2.  High-frequency conjugal plasmid transfer from gram-negative Escherichia coli to various gram-positive coryneform bacteria.

Authors:  A Schäfer; J Kalinowski; R Simon; A H Seep-Feldhaus; A Pühler
Journal:  J Bacteriol       Date:  1990-03       Impact factor: 3.490

3.  Transduction of plasmid DNA in Streptomyces spp. and related genera by bacteriophage FP43.

Authors:  M A McHenney; R H Baltz
Journal:  J Bacteriol       Date:  1988-05       Impact factor: 3.490

4.  Molecular analysis of a beta-lactam resistance gene encoded within the cephamycin gene cluster of Streptomyces clavuligerus.

Authors:  A S Paradkar; K A Aidoo; A Wong; S E Jensen
Journal:  J Bacteriol       Date:  1996-11       Impact factor: 3.490

5.  Increased fertility of Corynebacterium glutamicum recipients in intergeneric matings with Escherichia coli after stress exposure.

Authors:  A Schäfer; J Kalinowski; A Pühler
Journal:  Appl Environ Microbiol       Date:  1994-02       Impact factor: 4.792

6.  Cloning and characterization of a DNA region encoding a stress-sensitive restriction system from Corynebacterium glutamicum ATCC 13032 and analysis of its role in intergeneric conjugation with Escherichia coli.

Authors:  A Schäfer; A Schwarzer; J Kalinowski; A Pühler
Journal:  J Bacteriol       Date:  1994-12       Impact factor: 3.490

7.  Molecular cloning with a pMEA300-derived shuttle vector and characterization of the Amycolatopsis methanolica prephenate dehydratase gene.

Authors:  J W Vrijbloed; J van Hylckama Vlieg; N M van der Put; G I Hessels; L Dijkhuizen
Journal:  J Bacteriol       Date:  1995-11       Impact factor: 3.490

8.  Streptomyces lipmanii expresses two restriction systems that inhibit plasmid transformation and bacteriophage plaque formation.

Authors:  P Matsushima; R H Baltz
Journal:  J Bacteriol       Date:  1989-06       Impact factor: 3.490

9.  Precursor flux control through targeted chromosomal insertion of the lysine epsilon-aminotransferase (lat) gene in cephamycin C biosynthesis.

Authors:  L H Malmberg; W S Hu; D H Sherman
Journal:  J Bacteriol       Date:  1993-11       Impact factor: 3.490

10.  Investigation of the Streptomyces clavuligerus cephamycin C gene cluster and its regulation by the CcaR protein.

Authors:  D C Alexander; S E Jensen
Journal:  J Bacteriol       Date:  1998-08       Impact factor: 3.490
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.