Andrey G Sacramento1, Miriam R Fernandes2, Fábio P Sellera3, Maria E Muñoz2, Roberto Vivas4, Silvio S Dolabella4, Nilton Lincopan5. 1. Department of Morphology, Federal University of Sergipe, São Cristóvão, Brazil; Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: sacramento@usp.br. 2. Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil. 3. Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil. 4. Department of Morphology, Federal University of Sergipe, São Cristóvão, Brazil. 5. Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: lincopan@usp.br.
Abstract
OBJECTIVES: Genomic surveillance studies monitoring the dissemination of multidrug-resistant Enterobacteriaceae in polluted aquatic ecosystems are urgently required. The aim of this study was to report the draft genome sequence of an MCR-1 and CTX-M-8 co-producing Escherichia coli isolated from a polluted mangrove ecosystem in Northeast Brazil. METHODS: Total genomic DNA was sequenced on an Illumina NextSeq platform and was assembled using CLC Genomics Workbench. The whole-genome sequence was evaluated through bioinformatics tools available from the Center of Genomic Epidemiology as well as additional in silico analysis. RESULTS: The genome size was calculated at 5089467bp, comprising a total of 5068 protein-coding sequences. The strain was assigned to sequence type 58 (ST58) and revealed the presence of mcr-1, blaCTX-M-8 and other clinically significant genes responsible for conferring resistance to colistin, β-lactams, trimethoprim and quinolones. In addition, genes conferring resistance to silver (silR) and quaternary ammonium compounds (sugE) were identified. CONCLUSION: These data provide valuable information for comparative genomic analysis regarding the dissemination of MCR-1-producing E. coli at the human-animal-environment interface.
OBJECTIVES: Genomic surveillance studies monitoring the dissemination of multidrug-resistant Enterobacteriaceae in polluted aquatic ecosystems are urgently required. The aim of this study was to report the draft genome sequence of an MCR-1 and CTX-M-8 co-producing Escherichia coli isolated from a polluted mangrove ecosystem in Northeast Brazil. METHODS: Total genomic DNA was sequenced on an Illumina NextSeq platform and was assembled using CLC Genomics Workbench. The whole-genome sequence was evaluated through bioinformatics tools available from the Center of Genomic Epidemiology as well as additional in silico analysis. RESULTS: The genome size was calculated at 5089467bp, comprising a total of 5068 protein-coding sequences. The strain was assigned to sequence type 58 (ST58) and revealed the presence of mcr-1, blaCTX-M-8 and other clinically significant genes responsible for conferring resistance to colistin, β-lactams, trimethoprim and quinolones. In addition, genes conferring resistance to silver (silR) and quaternary ammonium compounds (sugE) were identified. CONCLUSION: These data provide valuable information for comparative genomic analysis regarding the dissemination of MCR-1-producing E. coli at the human-animal-environment interface.
Authors: Camilla Smoglica; Simone Angelucci; Muhammad Farooq; Antonio Antonucci; Fulvio Marsilio; Cristina E Di Francesco Journal: One Health Date: 2022-05-21
Authors: Ethan R Wyrsch; Piklu Roy Chowdhury; Louise Wallis; Max L Cummins; Tiziana Zingali; Kate J Brandis; Steven P Djordjevic Journal: Microb Genom Date: 2020-06-10
Authors: Cameron J Reid; Max L Cummins; Stefan Börjesson; Michael S M Brouwer; Henrik Hasman; Anette M Hammerum; Louise Roer; Stefanie Hess; Thomas Berendonk; Kristina Nešporová; Marisa Haenni; Jean-Yves Madec; Astrid Bethe; Geovana B Michael; Anne-Kathrin Schink; Stefan Schwarz; Monika Dolejska; Steven P Djordjevic Journal: Nat Commun Date: 2022-02-03 Impact factor: 17.694