Meng-Feng Wu1,2,3, Yun-Chi Wang1, Hsin-Ting Li1,4, Wei-Chun Chen1,5,6, Cheng-Hsi Liao1, Tzu-Ching Shih4, Wen-Shin Chang1, Chia-Wen Tsai7, Te-Chun Hsia7,5,6, DA-Tian Bau7,8,9. 1. Terry Fox Cancer Research Laboratory, Translational Medicine Research Center, China Medical University Hospital, Taichung, Taiwan, R.O.C. 2. Division of Chest Surgery, Department of Surgery, Taoyuan Armed Forces General Hospital, Taoyuan, Taiwan, R.O.C. 3. National Defense Medical Center, Taipei, Taiwan, R.O.C. 4. Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung, Taiwan, R.O.C. 5. Department of Respiratory Therapy, China Medical University, Taichung, Taiwan, R.O.C. 6. Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, China Medical University Hospital, Taichung, Taiwan, R.O.C. 7. Terry Fox Cancer Research Laboratory, Translational Medicine Research Center, China Medical University Hospital, Taichung, Taiwan, R.O.C. datian@mail.cmuh.org.tw artbau2@gmail.com. 8. Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, R.O.C. 9. Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan, R.O.C.
Abstract
BACKGROUND/AIM: Lung cancer is the leading cause of cancer-related death and a better marker for advanced personalized therapeutic approaches, such as immunotherapies, is in urgent need. Interleukin-12 (IL-12) is a cytokine that has been reported to exhibit potent tumoricidal effects, however, the contribution of IL-12 genotypes to lung cancer is still largely unrevealed. The aim of this study was to investigate whether single nucleotide polymorphisms (SNPs) in IL-12A and IL-12B are associated with lung cancer in a Taiwanese population. MATERIALS AND METHODS: Genotypes of 358 lung cancer patients and 716 controls were determined by the polymerase chain reaction-restriction fragment length polymorphism method. RESULTS: The distributions of genotypic (p=0.0036) and allelic (p=0.0005) frequencies of IL-12A rs568408 demonstrated significant differences between cases and controls. In detail, the AA genotype of IL-12A rs568408 was associated with a significantly elevated risk of lung cancer compared with the GG genotype (odds ratio(OR)=2.41, 95% confidence interval(CI)=1.36-4.29, p=0.0021). No difference was observed regarding IL-12A rs2243115 and IL-12B rs3212227 genotypes between the case and control groups. In addition, the results of interaction analysis showed that the AA genotype of IL-12A rs568408 was associated with elevated lung cancer risk, especially among those with smoking habits (p=0.0043). CONCLUSION: IL-12A rs568404 AA genotype may contribute to the etiology and serve as a genomic determinant of lung cancer in Taiwanese, especially smokers. Copyright
BACKGROUND/AIM: Lung cancer is the leading cause of cancer-related death and a better marker for advanced personalized therapeutic approaches, such as immunotherapies, is in urgent need. Interleukin-12 (IL-12) is a cytokine that has been reported to exhibit potent tumoricidal effects, however, the contribution of IL-12 genotypes to lung cancer is still largely unrevealed. The aim of this study was to investigate whether single nucleotide polymorphisms (SNPs) in IL-12A and IL-12B are associated with lung cancer in a Taiwanese population. MATERIALS AND METHODS: Genotypes of 358 lung cancerpatients and 716 controls were determined by the polymerase chain reaction-restriction fragment length polymorphism method. RESULTS: The distributions of genotypic (p=0.0036) and allelic (p=0.0005) frequencies of IL-12Ars568408 demonstrated significant differences between cases and controls. In detail, the AA genotype of IL-12Ars568408 was associated with a significantly elevated risk of lung cancer compared with the GG genotype (odds ratio(OR)=2.41, 95% confidence interval(CI)=1.36-4.29, p=0.0021). No difference was observed regarding IL-12Ars2243115 and IL-12Brs3212227 genotypes between the case and control groups. In addition, the results of interaction analysis showed that the AA genotype of IL-12Ars568408 was associated with elevated lung cancer risk, especially among those with smoking habits (p=0.0043). CONCLUSION:IL-12Ars568404 AA genotype may contribute to the etiology and serve as a genomic determinant of lung cancer in Taiwanese, especially smokers. Copyright