Literature DB >> 30394128

Capture and storage of plant genomic DNA on a readily available cellulose matrix.

Megan M Thompson1, Estelle M Hrabak1.   

Abstract

Whatman FTA® Cards are a fast and efficient method for capturing and storing nucleic acids but can be cost-prohibitive for large numbers of samples. We developed a method that substitutes a readily-available cellulose matrix and homemade washing buffer for commercial FTA® Cards and FTA® Purification Reagent. This method is suitable for long-term storage of DNA from many plant species prior to PCR.

Entities:  

Keywords:  DNA; FTA purification reagent; FTA® Cards; PCR; polymerase chain reaction

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Year:  2018        PMID: 30394128     DOI: 10.2144/btn-2018-0109

Source DB:  PubMed          Journal:  Biotechniques        ISSN: 0736-6205            Impact factor:   1.993


  2 in total

1.  FTA-Sodium hydroxide-based polymerase chain reaction (PCR): An efficient and cheaper option for Theileria parva detection in dairy cattle in Mbarara, Uganda.

Authors:  Leo Uchida; Joseph Byaruhanga; Ikuo Okamura; Takeshi Miyama; Yasukazu Muramatsu; Patrick Vudriko; Kohei Makita
Journal:  J Vet Med Sci       Date:  2019-12-25       Impact factor: 1.267

2.  Multiplexed colorimetric detection of SARS-CoV-2 and other pathogens in wastewater on a 3D printed integrated microfluidic chip.

Authors:  Kun Yin; Xiong Ding; Zhiheng Xu; Ziyue Li; Xingyu Wang; Hui Zhao; Clifford Otis; Baikun Li; Changchun Liu
Journal:  Sens Actuators B Chem       Date:  2021-06-07       Impact factor: 7.460

  2 in total

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