Fabian Frost1, Tim Kacprowski2, Malte Rühlemann3, Robin Bülow4, Jens-Peter Kühn5, Andre Franke3, Femke-Anouska Heinsen3, Maik Pietzner6, Matthias Nauck7, Uwe Völker8, Henry Völzke9, Ali A Aghdassi1, Matthias Sendler1, Julia Mayerle10, Frank U Weiss1, Georg Homuth8, Markus M Lerch11. 1. Department of Medicine A, University Medicine Greifswald, Greifswald, Germany. 2. Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany; Chair of Experimental Bioinformatics, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany; DZHK (German Centre for Cardiovascular Research), Partner Site Greifswald, University Medicine Greifswald, Greifswald, Germany. 3. Institute of Clinical Molecular Biology, Christian Albrechts University of Kiel, Kiel, Germany. 4. Department of Diagnostic Radiology and Neuroradiology, University Medicine Greifswald, Greifswald, Germany. 5. Department of Diagnostic Radiology and Neuroradiology, University Medicine Greifswald, Greifswald, Germany; Institute of Radiology, University Medicine, Carl-Gustav-Carus University, Dresden, Germany. 6. Institute of Clinical Chemistry and Laboratory Medicine, University Medicine Greifswald, Greifswald, Germany. 7. DZHK (German Centre for Cardiovascular Research), Partner Site Greifswald, University Medicine Greifswald, Greifswald, Germany; Institute of Clinical Chemistry and Laboratory Medicine, University Medicine Greifswald, Greifswald, Germany. 8. Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany. 9. Institute for Community Medicine, University Medicine Greifswald, Greifswald, Germany. 10. Department of Medicine A, University Medicine Greifswald, Greifswald, Germany; Department of Medicine II, University Hospital, LMU Munich, Germany. 11. Department of Medicine A, University Medicine Greifswald, Greifswald, Germany. Electronic address: lerch@uni-greifswald.de.
Abstract
BACKGROUND & AIMS: Changes in intestinal microbiome composition are associated with inflammatory, metabolic, and malignant disorders. We studied how exocrine pancreatic function affects intestinal microbiota. METHODS: We performed 16S ribosomal RNA gene sequencing analysis of stool samples from 1795 volunteers from the population-based Study of Health in Pomerania who had no history of pancreatic disease. We also measured fecal pancreatic elastase by enzyme-linked immunosorbent assay and performed quantitative imaging of secretin-stimulated pancreatic fluid secretion. Associations of exocrine pancreatic function with microbial diversity or individual genera were calculated by permutational analysis of variance or linear regression, respectively. RESULTS: Differences in pancreatic elastase levels associated with significantly (P < .0001) greater changes in microbiota diversity than with participant age, body mass index, sex, smoking, alcohol consumption, or dietary factors. Significant changes in the abundance of 30 taxa, such as an increase in Prevotella (q < .0001) and a decrease of Bacteroides (q < .0001), indicated a shift from a type-1 to a type-2 enterotype. Changes in pancreatic fluid secretion alone were also associated with changes in microbial diversity (P = .0002), although to a lesser degree. CONCLUSIONS: In an analysis of fecal samples from 1795 volunteers, pancreatic acinar cell, rather than duct cell, function is presently the single most significant host factor to be associated with changes in intestinal microbiota composition.
BACKGROUND & AIMS: Changes in intestinal microbiome composition are associated with inflammatory, metabolic, and malignant disorders. We studied how exocrine pancreatic function affects intestinal microbiota. METHODS: We performed 16S ribosomal RNA gene sequencing analysis of stool samples from 1795 volunteers from the population-based Study of Health in Pomerania who had no history of pancreatic disease. We also measured fecal pancreatic elastase by enzyme-linked immunosorbent assay and performed quantitative imaging of secretin-stimulated pancreatic fluid secretion. Associations of exocrine pancreatic function with microbial diversity or individual genera were calculated by permutational analysis of variance or linear regression, respectively. RESULTS: Differences in pancreatic elastase levels associated with significantly (P < .0001) greater changes in microbiota diversity than with participant age, body mass index, sex, smoking, alcohol consumption, or dietary factors. Significant changes in the abundance of 30 taxa, such as an increase in Prevotella (q < .0001) and a decrease of Bacteroides (q < .0001), indicated a shift from a type-1 to a type-2 enterotype. Changes in pancreatic fluid secretion alone were also associated with changes in microbial diversity (P = .0002), although to a lesser degree. CONCLUSIONS: In an analysis of fecal samples from 1795 volunteers, pancreatic acinar cell, rather than duct cell, function is presently the single most significant host factor to be associated with changes in intestinal microbiota composition.
Authors: Malte Christoph Rühlemann; Britt Marie Hermes; Corinna Bang; Shauni Doms; Lucas Moitinho-Silva; Louise Bruun Thingholm; Fabian Frost; Frauke Degenhardt; Michael Wittig; Jan Kässens; Frank Ulrich Weiss; Annette Peters; Klaus Neuhaus; Uwe Völker; Henry Völzke; Georg Homuth; Stefan Weiss; Harald Grallert; Matthias Laudes; Wolfgang Lieb; Dirk Haller; Markus M Lerch; John F Baines; Andre Franke Journal: Nat Genet Date: 2021-01-18 Impact factor: 38.330
Authors: Louise B Thingholm; Malte C Rühlemann; Manja Koch; Brie Fuqua; Guido Laucke; Ruwen Boehm; Corinna Bang; Eric A Franzosa; Matthias Hübenthal; Ali Rahnavard; Fabian Frost; Jason Lloyd-Price; Melanie Schirmer; Aldons J Lusis; Chris D Vulpe; Markus M Lerch; Georg Homuth; Tim Kacprowski; Carsten O Schmidt; Ute Nöthlings; Tom H Karlsen; Wolfgang Lieb; Matthias Laudes; Andre Franke; Curtis Huttenhower Journal: Cell Host Microbe Date: 2019-08-06 Impact factor: 21.023
Authors: Bara El Kurdi; Sumbal Babar; Mahmoud El Iskandarani; Adam Bataineh; Markus M Lerch; Mark Young; Vijay P Singh Journal: Clin Transl Gastroenterol Date: 2019-09 Impact factor: 4.488
Authors: C E Pope; A T Vo; H S Hayden; E J Weiss; S Durfey; S McNamara; A Ratjen; B Grogan; S Carter; L Nay; M R Parsek; P K Singh; E F McKone; M L Aitken; M R Rosenfeld; L R Hoffman Journal: J Cyst Fibros Date: 2020-12-31 Impact factor: 5.527
Authors: Fabian Frost; Frank U Weiss; Matthias Sendler; Tim Kacprowski; Malte Rühlemann; Corinna Bang; Andre Franke; Uwe Völker; Henry Völzke; Georg Lamprecht; Julia Mayerle; Ali A Aghdassi; Georg Homuth; Markus M Lerch Journal: Clin Transl Gastroenterol Date: 2020-09 Impact factor: 4.396