Literature DB >> 3037940

A continuous fluorescence assay for cyclic nucleotide phosphodiesterase hydrolysis of cyclic GMP.

J D Johnson, J D Walters, J S Mills.   

Abstract

The fluorescent 2'-methylanthraniloyl derivative of cyclic GMP undergoes a 45% decrease in fluorescence when it is cleaved by brain phosphodiesterase in the presence of calmodulin. This fluorescence decrease is dependent upon calcium, calmodulin, and phosphodiesterase, and correlates well (r = 0.996) with the disappearance of substrate as monitored by high-performance liquid chromatography. The Kd values determined by this fluorescence method and HPLC suggest that cyclic GMP and its fluorescent derivative exhibit similar kinetic parameters in their hydrolysis.

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Year:  1987        PMID： 3037940     DOI： 10.1016/0003-2697(87)90039-x

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  8 in total

1.  Activation of Trp3 by inositol 1,4,5-trisphosphate receptors through displacement of inhibitory calmodulin from a common binding domain.

Authors:  Z Zhang; J Tang; S Tikunova; J D Johnson; Z Chen; N Qin; A Dietrich; E Stefani; L Birnbaumer; M X Zhu
Journal:  Proc Natl Acad Sci U S A       Date:  2001-02-27       Impact factor: 11.205

2.  Effect of MANT-nucleotides on L-type calcium currents in murine cardiomyocytes.

Authors:  Melanie Hübner; Sara Dizayee; Jan Matthes; Roland Seifert; Stefan Herzig
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  2011-04-12       Impact factor: 3.000

3.  Characterization of a novel cAMP-binding, cAMP-specific cyclic nucleotide phosphodiesterase (TcrPDEB1) from Trypanosoma cruzi.

Authors:  Rocío Díaz-Benjumea; Sunil Laxman; Thomas R Hinds; Joseph A Beavo; Ana Rascón
Journal:  Biochem J       Date:  2006-10-15       Impact factor: 3.857

4.  Differential regulation of Ca2+/calmodulin-dependent enzymes by plant calmodulin isoforms and free Ca2+ concentration.

Authors:  S H Lee; J D Johnson; M P Walsh; J E Van Lierop; C Sutherland; A Xu; W A Snedden; D Kosk-Kosicka; H Fromm; N Narayanan; M J Cho
Journal:  Biochem J       Date:  2000-08-15       Impact factor: 3.857

5.  Expression in bacteria of functional inhibitory subunit of retinal rod cGMP phosphodiesterase.

Authors:  R L Brown; L Stryer
Journal:  Proc Natl Acad Sci U S A       Date:  1989-07       Impact factor: 11.205

Review 6.  Assays of cyclic nucleotides. A review of current techniques.

Authors:  B J Smith; M R Wales; M J Perry
Journal:  Appl Biochem Biotechnol       Date:  1993-06       Impact factor: 2.926

7.  Phosphorylation by casein kinase II alters the biological activity of calmodulin.

Authors:  D B Sacks; H W Davis; J P Williams; E L Sheehan; J G Garcia; J M McDonald
Journal:  Biochem J       Date:  1992-04-01       Impact factor: 3.857

8.  Analysis of substrate specificity and kinetics of cyclic nucleotide phosphodiesterases with N'-methylanthraniloyl-substituted purine and pyrimidine 3',5'-cyclic nucleotides by fluorescence spectrometry.

Authors:  Daniel Reinecke; Frank Schwede; Hans-Gottfried Genieser; Roland Seifert
Journal:  PLoS One       Date:  2013-01-14       Impact factor: 3.240
  8 in total

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