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Literature DB >> 8379663

Assays of cyclic nucleotides. A review of current techniques.

Abstract

Cyclic nucleotides are recognized as important second messenger molecules, and many assay formats exist for their quantification. This article critically reviews these different approaches. For measurement of cAMP or cGMP in biological fluids or tissue extracts, immunoassay is effective. For other purposes, such as measurement of cyclic nucleotide phosphodiesterase activity, methods that separate nucleotides from their cyclic counterparts are best, and offer a variety of means of detection.

Entities: Chemical

Mesh：

Substances：
Nucleotides, Cyclic

Year: 1993 PMID： 8379663 DOI： 10.1007/bf02916422

Source DB: PubMed Journal: Appl Biochem Biotechnol ISSN： 0273-2289 Impact factor: 2.926

44 in total

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Authors: U B Kaupp
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Authors: S R Adams; A T Harootunian; Y J Buechler; S S Taylor; R Y Tsien
Journal: Nature Date: 1991-02-21 Impact factor: 49.962

3 in total

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2. Signaling-sensitive amino acids surround the allosteric ligand binding site of the thyrotropin receptor.

Authors: Gunnar Kleinau; Ann-Karin Haas; Susanne Neumann; Catherine L Worth; Inna Hoyer; Jens Furkert; Claudia Rutz; Marvin C Gershengorn; Ralf Schülein; Gerd Krause
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3. From molecular details of the interplay between transmembrane helices of the thyrotropin receptor to general aspects of signal transduction in family a G-protein-coupled receptors (GPCRs).

Authors: Gunnar Kleinau; Inna Hoyer; Annika Kreuchwig; Ann-Karin Haas; Claudia Rutz; Jens Furkert; Catherine L Worth; Gerd Krause; Ralf Schülein
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3 in total