Literature DB >> 30377366

The hit-and-return system enables efficient time-resolved serial synchrotron crystallography.

Eike C Schulz1, Pedram Mehrabi1, Henrike M Müller-Werkmeister1,2, Friedjof Tellkamp3, Ajay Jha1, William Stuart1, Elke Persch4, Raoul De Gasparo4, François Diederich4, Emil F Pai5,6,7,8, R J Dwayne Miller9,10,11.   

Abstract

We present a 'hit-and-return' (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.

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Year:  2018        PMID： 30377366     DOI： 10.1038/s41592-018-0180-2

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  17 in total

1.  The crystal structures of a chloride-pumping microbial rhodopsin and its proton-pumping mutant illuminate proton transfer determinants.

Authors:  Jessica E Besaw; Wei-Lin Ou; Takefumi Morizumi; Bryan T Eger; Juan D Sanchez Vasquez; Jessica H Y Chu; Andrew Harris; Leonid S Brown; R J Dwayne Miller; Oliver P Ernst
Journal:  J Biol Chem       Date:  2020-07-23       Impact factor: 5.157

2.  Serial macromolecular crystallography at ALBA Synchrotron Light Source.

Authors:  Jose M Martin-Garcia; Sabine Botha; Hao Hu; Rebecca Jernigan; Albert Castellví; Stella Lisova; Fernando Gil; Barbara Calisto; Isidro Crespo; Shatabdi Roy-Chowdhury; Alice Grieco; Gihan Ketawala; Uwe Weierstall; John Spence; Petra Fromme; Nadia Zatsepin; Dirk Roeland Boer; Xavi Carpena
Journal:  J Synchrotron Radiat       Date:  2022-04-04       Impact factor: 2.557

3.  High-throughput structures of protein-ligand complexes at room temperature using serial femtosecond crystallography.

Authors:  Tadeo Moreno-Chicano; Ali Ebrahim; Danny Axford; Martin V Appleby; John H Beale; Amanda K Chaplin; Helen M E Duyvesteyn; Reza A Ghiladi; Shigeki Owada; Darren A Sherrell; Richard W Strange; Hiroshi Sugimoto; Kensuke Tono; Jonathan A R Worrall; Robin L Owen; Michael A Hough
Journal:  IUCrJ       Date:  2019-10-10       Impact factor: 4.769

4.  Serial femtosecond and serial synchrotron crystallography can yield data of equivalent quality: A systematic comparison.

Authors:  P Mehrabi; R Bücker; G Bourenkov; H M Ginn; D von Stetten; H M Müller-Werkmeister; A Kuo; T Morizumi; B T Eger; W-L Ou; S Oghbaey; A Sarracini; J E Besaw; O Pare-Labrosse; S Meier; H Schikora; F Tellkamp; A Marx; D A Sherrell; D Axford; R L Owen; O P Ernst; E F Pai; E C Schulz; R J D Miller
Journal:  Sci Adv       Date:  2021-03-17       Impact factor: 14.136

5.  Evaluation of the data-collection strategy for room-temperature micro-crystallography studied by serial synchrotron rotation crystallography combined with the humid air and glue-coating method.

Authors:  Kazuya Hasegawa; Seiki Baba; Takashi Kawamura; Masaki Yamamoto; Takashi Kumasaka
Journal:  Acta Crystallogr D Struct Biol       Date:  2021-02-25       Impact factor: 7.652

6.  Fixed-target serial femtosecond crystallography using in cellulo grown microcrystals.

Authors:  J Mia Lahey-Rudolph; Robert Schönherr; Miriam Barthelmess; Pontus Fischer; Carolin Seuring; Armin Wagner; Alke Meents; Lars Redecke
Journal:  IUCrJ       Date:  2021-06-18       Impact factor: 4.769

7.  High-throughput in situ experimental phasing.

Authors:  Joshua M Lawrence; Julien Orlans; Gwyndaf Evans; Allen M Orville; James Foadi; Pierre Aller
Journal:  Acta Crystallogr D Struct Biol       Date:  2020-07-28       Impact factor: 7.652

8.  The HARE chip for efficient time-resolved serial synchrotron crystallography.

Authors:  Pedram Mehrabi; Henrike M Müller-Werkmeister; Jan Philipp Leimkohl; Hendrik Schikora; Jelena Ninkovic; Silvia Krivokuca; Ladislav Andriček; Sascha W Epp; Darren Sherrell; Robin L Owen; Arwen R Pearson; Friedjof Tellkamp; Eike C Schulz; R J Dwayne Miller
Journal:  J Synchrotron Radiat       Date:  2020-02-27       Impact factor: 2.616

9.  Radiation damage and dose limits in serial synchrotron crystallography at cryo- and room temperatures.

Authors:  Eugenio de la Mora; Nicolas Coquelle; Charles S Bury; Martin Rosenthal; James M Holton; Ian Carmichael; Elspeth F Garman; Manfred Burghammer; Jacques-Philippe Colletier; Martin Weik
Journal:  Proc Natl Acad Sci U S A       Date:  2020-02-11       Impact factor: 11.205

10.  A fixed-target platform for serial femtosecond crystallography in a hydrated environment.

Authors:  M L Shelby; D Gilbile; T D Grant; C Seuring; B W Segelke; W He; A C Evans; T Pakendorf; P Fischer; M S Hunter; A Batyuk; M Barthelmess; A Meents; M A Coleman; T L Kuhl; M Frank
Journal:  IUCrJ       Date:  2020-01-01       Impact factor: 4.769
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