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Literature DB >> 30376607

Identification of Specific and Nonspecific Inhibitors of Bacillus anthracis Type III Pantothenate Kinase (PanK).

Justin A Shapiro¹, John J Varga², Derek Parsonage³, William Walton⁴, Matthew R Redinbo⁴, Larry J Ross⁵, E Lucile White⁵, Robert Bostwick⁵, William M Wuest^1,6, Al Claiborne³, Joanna B Goldberg^2,6.

Abstract

Antibiotics with novel mechanisms of action are desperately needed to combat the increasing rates of multidrug-resistant infections. Bacterial pantothenate kinase (PanK) has emerged as a target of interest to cut off the biosynthesis of coenzyme A. Herein we report the results of an in vitro high-throughput screen of over 10 000 small molecules against Bacillus anthracis PanK, as well as a follow-up screen of hits against PanK isolated from Pseudomonas aeruginosa and Burkholderia cenocepacia. Nine hits are structurally categorized and analyzed to set the stage for future drug development.

Entities: CellLine Chemical Disease Gene Species

Keywords: Bacillus; Pseudomonas; antibiotic resistance; high-throughput screening; pantothenate kinase

Mesh：

Substances：

Year: 2018 PMID： 30376607 PMCID： PMC6456334 DOI： 10.1002/cmdc.201800652

Source DB: PubMed Journal: ChemMedChem ISSN： 1860-7179 Impact factor: 3.466

11 in total

1. A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site.

Authors: Lucy Stols; Minyi Gu; Lynda Dieckman; Rosemarie Raffen; Frank R Collart; Mark I Donnelly
Journal: Protein Expr Purif Date: 2002-06 Impact factor: 1.650

2. NIH Molecular Libraries Initiative.

Authors: Christopher P Austin; Linda S Brady; Thomas R Insel; Francis S Collins
Journal: Science Date: 2004-11-12 Impact factor: 47.728

3. Crystal structure of a type III pantothenate kinase: insight into the mechanism of an essential coenzyme A biosynthetic enzyme universally distributed in bacteria.

Authors: Kun Yang; Yvonne Eyobo; Leisl A Brand; Dariusz Martynowski; Diana Tomchick; Erick Strauss; Hong Zhang
Journal: J Bacteriol Date: 2006-08 Impact factor: 3.490

4. Prokaryotic type II and type III pantothenate kinases: The same monomer fold creates dimers with distinct catalytic properties.

Authors: Bum Soo Hong; Mi Kyung Yun; Yong-Mei Zhang; Shigeru Chohnan; Charles O Rock; Stephen W White; Suzanne Jackowski; Hee-Won Park; Roberta Leonardi
Journal: Structure Date: 2006-08 Impact factor: 5.006

5. Structure of the type III pantothenate kinase from Bacillus anthracis at 2.0 A resolution: implications for coenzyme A-dependent redox biology.

Authors: Nathan I Nicely; Derek Parsonage; Carleitta Paige; Gerald L Newton; Robert C Fahey; Roberta Leonardi; Suzanne Jackowski; T Conn Mallett; Al Claiborne
Journal: Biochemistry Date: 2007-02-27 Impact factor: 3.162

6. Protein production by auto-induction in high density shaking cultures.

Authors: F William Studier
Journal: Protein Expr Purif Date: 2005-05 Impact factor: 1.650

Review 7. Coenzyme A biosynthesis: an antimicrobial drug target.

Authors: Christina Spry; Kiaran Kirk; Kevin J Saliba
Journal: FEMS Microbiol Rev Date: 2008-01 Impact factor: 16.408

8. Inhibitors of pantothenate kinase: novel antibiotics for staphylococcal infections.

Authors: Anthony E Choudhry; Tracy L Mandichak; John P Broskey; Richard W Egolf; Cynthia Kinsland; Tadhg P Begley; Mark A Seefeld; Thomas W Ku; James R Brown; Magdalena Zalacain; Kapila Ratnam
Journal: Antimicrob Agents Chemother Date: 2003-06 Impact factor: 5.191