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Literature DB >> 3037345

Structure of two developmentally regulated Dictyostelium discoideum ubiquitin genes.

Abstract

A previously isolated cDNA clone, pLK229, that is specific for mRNA developmentally expressed during Dictyostelium discoideum spore germination and multicellular development, was used to screen two genomic libraries. Two genomic sequences homologous to pLK229 were isolated and sequenced. Genomic clone p229 is identical to the cDNA clone pLK229 and codes for a polypeptide of 381 amino acids. This polypeptide is composed of five tandem repeats of the same 76-amino-acid sequence. Clone lambda 229 codes for a protein of 229 amino acids, containing three tandem repeats of the identical 76-amino-acid sequence. A computer search for homology to known proteins revealed that the 76-amino-acid repeat was identical to human and bovine ubiquitin except for two amino acid differences.

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Year: 1987 PMID： 3037345 PMCID： PMC365330 DOI： 10.1128/mcb.7.6.2097-2103.1987

Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 4.272

30 in total

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15 in total

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Journal: Nucleic Acids Res Date: 1989-08-11 Impact factor: 16.971

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Journal: Mol Gen Genet Date: 1988-08

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Journal: Mol Cell Biol Date: 1988-11 Impact factor: 4.272

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Journal: Nucleic Acids Res Date: 1995-08-11 Impact factor: 16.971

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Journal: Nucleic Acids Res Date: 1991-03-11 Impact factor: 16.971