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Literature DB >> 2549509

Ubiquitin expression in Neurospora crassa: cloning and sequencing of a polyubiquitin gene.

G E Taccioli¹, E Grotewold, G O Aisemberg, N D Judewicz.

Abstract

We have cloned and sequenced a polyubiquitin gene from Neurospora crassa that is organized in a four repeat-tandem array. The first repeat contains a small intron and the last is fused to an extra glutamine codon. In Northern blots, two RNA species of 1.3 kb and 0.7 kb hybridize to the isolated clone. The larger ubiquitin (UBI) transcript accumulates after partial inhibition of protein synthesis with cycloheximide, and the smaller one preferentially accumulates in conidia after germination. Unexpectedly, constitutive expression of UBI transcripts in exponentially grown mycelia is not altered by heat-shock or exposure to arsenite.

Entities: Chemical Disease Species

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Year: 1989 PMID： 2549509 PMCID： PMC318268 DOI： 10.1093/nar/17.15.6153

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

54 in total

Review 1. Biochemical genetics of Neurospora crassa conidial germination.

Authors: J C Schmit; S Brody
Journal: Bacteriol Rev Date: 1976-03

2. A general method for isolation of high molecular weight DNA from eukaryotes.

Authors: N Blin; D W Stafford
Journal: Nucleic Acids Res Date: 1976-09 Impact factor: 16.971

3. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.

Authors: H Lehrach; D Diamond; J M Wozney; H Boedtker
Journal: Biochemistry Date: 1977-10-18 Impact factor: 3.162

4. Structure and activities of a variant ubiquitin sequence from bakers' yeast.

Authors: K D Wilkinson; M J Cox; L B O'Connor; R Shapira
Journal: Biochemistry Date: 1986-09-09 Impact factor: 3.162

5. Structure of the receptor for platelet-derived growth factor helps define a family of closely related growth factor receptors.

Authors: Y Yarden; J A Escobedo; W J Kuang; T L Yang-Feng; T O Daniel; P M Tremble; E Y Chen; M E Ando; R N Harkins; U Francke
Journal: Nature Date: 1986 Sep 18-24 Impact factor: 49.962

6. A Neurospora crassa morphological mutant showing reduced adenylate cyclase activity.

Authors: H F Terenzi; M M Flawiá; H N Torres
Journal: Biochem Biophys Res Commun Date: 1974-06-18 Impact factor: 3.575

7. Different intracellular distributions of heat-shock and arsenite-induced proteins in Drosophila Kc cells. Possible relation with the phosphorylation and translocation of a major cytoskeletal protein.

Authors: M Vincent; R M Tanguay
Journal: J Mol Biol Date: 1982-12-05 Impact factor: 5.469

8. Disappearance of a structural chromatin protein A24 in mitosis: implications for molecular basis of chromatin condensation.

Authors: S I Matsui; B K Seon; A A Sandberg
Journal: Proc Natl Acad Sci U S A Date: 1979-12 Impact factor: 11.205

5. The intron of Arabidopsis thaliana polyubiquitin genes is conserved in location and is a quantitative determinant of chimeric gene expression.

Authors: S R Norris; S E Meyer; J Callis
Journal: Plant Mol Biol Date: 1993-03 Impact factor: 4.076

6. New nucleotide sequence data on the EMBL File Server.

Authors:
Journal: Nucleic Acids Res Date: 1989-09-25 Impact factor: 16.971

6 in total

Ubiquitin expression in Neurospora crassa: cloning and sequencing of a polyubiquitin gene.

Review 1. Biochemical genetics of Neurospora crassa conidial germination.

2. A general method for isolation of high molecular weight DNA from eukaryotes.

3. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.

4. Structure and activities of a variant ubiquitin sequence from bakers' yeast.

5. Structure of the receptor for platelet-derived growth factor helps define a family of closely related growth factor receptors.

6. A Neurospora crassa morphological mutant showing reduced adenylate cyclase activity.

7. Different intracellular distributions of heat-shock and arsenite-induced proteins in Drosophila Kc cells. Possible relation with the phosphorylation and translocation of a major cytoskeletal protein.

8. Disappearance of a structural chromatin protein A24 in mitosis: implications for molecular basis of chromatin condensation.

9. Selective arrangement of ubiquitinated and D1 protein-containing nucleosomes within the Drosophila genome.

10. Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown.

1. A novel form of ubiquitin found in the basidiomycete fungus, Coprinus congregatus.

2. Expression of ubiquitin gene in Microsporum canis and Trichophyton mentagrophytes cultured with fluconazole.

3. Isolation and characterization of mutants defective in production of laccase in Neurospora crassa.

4. Repetitious structure and transcription control of a polyubiquitin gene in Volvox carteri.

5. The intron of Arabidopsis thaliana polyubiquitin genes is conserved in location and is a quantitative determinant of chimeric gene expression.

6. New nucleotide sequence data on the EMBL File Server.