Manzhu Zhao1, Xiujie Wen2, Gang Li2, Yingxin Ju2, Yingying Wang2, Zhi Zhou1, Jinlin Song1. 1. College of Stomatology, Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing Medical University, Chongqing, China. 2. Department of Stomatology, Daping Hospital & Research Institute of Surgery, Third Military Medical University, Chongqing, China.
Abstract
OBJECTIVE: The aim of this study was to investigate the spatiotemporal expression and potential role of p75NTR in tooth morphogenesis and tissue mineralization. MATERIALS AND METHODS: The dynamic morphology of the four stages (from the beginning of E12.5 d, then E13.5 d and E15.5 d, to the end of E18.5 d) was observed, and the expressions of p75NTR and Runx2 were traced. The ectomesenchymal stem cells (EMSCs) were harvested in vitro, and the biological characteristics were observed. Moreover, the mineralization capability of EMSCs was evaluated. The relations between p75NTR and ALP, Col-1 and Runx2 were investigated. RESULTS: The morphologic results showed that the dental lamina appeared at E12.5 d, the bud stage at E13.5 d, the cap stage at E15.5 d and the bell stage at E18.5 d. p75NTR and Runx2 showed the similar expression pattern. EMSCs from the four stages showed no significant difference in proliferation. But the positive rate of p75NTR in the E12.5 d cells was significantly lower than that in the other three stages (P < 0.05). Moreover, the higher positive rate of p75NTR the cells were, the stronger mineralization capability they showed. p75NTR was well positively correlated with the mineralization-related markers ALP, Col-1 and Runx2, which increased gradually with the mature of dental germs. CONCLUSION: p75NTR might play an important role in the regulation of tooth morphogenesis, especially dental hard tissue formation.
OBJECTIVE: The aim of this study was to investigate the spatiotemporal expression and potential role of p75NTR in tooth morphogenesis and tissue mineralization. MATERIALS AND METHODS: The dynamic morphology of the four stages (from the beginning of E12.5 d, then E13.5 d and E15.5 d, to the end of E18.5 d) was observed, and the expressions of p75NTR and Runx2 were traced. The ectomesenchymal stem cells (EMSCs) were harvested in vitro, and the biological characteristics were observed. Moreover, the mineralization capability of EMSCs was evaluated. The relations between p75NTR and ALP, Col-1 and Runx2 were investigated. RESULTS: The morphologic results showed that the dental lamina appeared at E12.5 d, the bud stage at E13.5 d, the cap stage at E15.5 d and the bell stage at E18.5 d. p75NTR and Runx2 showed the similar expression pattern. EMSCs from the four stages showed no significant difference in proliferation. But the positive rate of p75NTR in the E12.5 d cells was significantly lower than that in the other three stages (P < 0.05). Moreover, the higher positive rate of p75NTR the cells were, the stronger mineralization capability they showed. p75NTR was well positively correlated with the mineralization-related markers ALP, Col-1 and Runx2, which increased gradually with the mature of dental germs. CONCLUSION: p75NTR might play an important role in the regulation of tooth morphogenesis, especially dental hard tissue formation.
Authors: Stefan Hauser; Darius Widera; Firas Qunneis; Janine Müller; Christin Zander; Johannes Greiner; Christina Strauss; Patrick Lüningschrör; Peter Heimann; Hartmut Schwarze; Jörg Ebmeyer; Holger Sudhoff; Marcos J Araúzo-Bravo; Boris Greber; Holm Zaehres; Hans Schöler; Christian Kaltschmidt; Barbara Kaltschmidt Journal: Stem Cells Dev Date: 2012-01-26 Impact factor: 3.272
Authors: Y Chai; X Jiang; Y Ito; P Bringas; J Han; D H Rowitch; P Soriano; A P McMahon; H M Sucov Journal: Development Date: 2000-04 Impact factor: 6.868