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Literature DB >> 3035498

1H NMR study of the exchangeable protons of the duplex d(GCGTTGCG).d(CGCAACGC) containing a thymine photodimer.

J Kemmink, R Boelens, T Koning, G A van der Marel, J H van Boom, R Kaptein.

Abstract

A comparison is presented of the imino proton NMR spectra of the double stranded octamer d(GCGTTGCG).d(CGCAACGC) and the same octamer in which the two central thymine residues occur as a cis-syn thymine dimer. Except for the terminal base pairs all imino protons were detected and assigned in the NMR spectrum. The spectra show that in the thymine dimer duplex, contrary to common belief, all base pairs occur in a hydrogen bonded form, although the hydrogen bonds of the two central AT base pairs are substantially weakened. The melting temperature decreases about 13 degrees C on thymine dimer formation.

Entities: Chemical Gene

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Year: 1987 PMID： 3035498 PMCID： PMC340886 DOI： 10.1093/nar/15.11.4645

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

6 in total

1. Disappearance of thymine photodimer in ultraviolet irradiated DNA upon treatment with a photoreactivating enzyme from baker's yeast.

Authors: D L WULFF; C S RUPERT
Journal: Biochem Biophys Res Commun Date: 1962-04-20 Impact factor: 3.575

2. Effect of a single thymine photodimer on the oligodeoxythymidylate-polydeoxyadenylate interaction.

Authors: F N Hayes; D L Williams; R L Ratlift; A J Varghese; C S Rupert
Journal: J Am Chem Soc Date: 1971-09 Impact factor: 15.419

3. Molecular models for DNA damaged by photoreaction.

Authors: D A Pearlman; S R Holbrook; D H Pirkle; S H Kim
Journal: Science Date: 1985-03-15 Impact factor: 47.728

4. The structure of d(CGCGAAT[]TCGCG) . d(CGCGAATTCGCG); the incorporation of a thymine photodimer into a B-DNA helix.

Authors: S N Rao; J W Keepers; P Kollman
Journal: Nucleic Acids Res Date: 1984-06-11 Impact factor: 16.971

5. Base-base mismatches. Thermodynamics of double helix formation for dCA3XA3G + dCT3YT3G (X, Y = A,C,G,T).

Authors: F Aboul-ela; D Koh; I Tinoco; F H Martin
Journal: Nucleic Acids Res Date: 1985-07-11 Impact factor: 16.971

6. Hairpin formation in synthetic oligonucleotides.

Authors: C W Hilbers; C A Haasnoot; S H de Bruin; J J Joordens; G A van der Marel; J H van Boom
Journal: Biochimie Date: 1985 Jul-Aug Impact factor: 4.079

6 in total

32 in total

1. Escherichia coli DNA polymerase III can replicate efficiently past a T-T cis-syn cyclobutane dimer if DNA polymerase V and the 3' to 5' exonuclease proofreading function encoded by dnaQ are inactivated.

Authors: Angela Borden; Paul I O'Grady; Dominique Vandewiele; Antonio R Fernández de Henestrosa; Christopher W Lawrence; Roger Woodgate
Journal: J Bacteriol Date: 2002-05 Impact factor: 3.490

1H NMR study of the exchangeable protons of the duplex d(GCGTTGCG).d(CGCAACGC) containing a thymine photodimer.

1. Disappearance of thymine photodimer in ultraviolet irradiated DNA upon treatment with a photoreactivating enzyme from baker's yeast.

2. Effect of a single thymine photodimer on the oligodeoxythymidylate-polydeoxyadenylate interaction.

3. Molecular models for DNA damaged by photoreaction.

4. The structure of d(CGCGAAT[]TCGCG) . d(CGCGAATTCGCG); the incorporation of a thymine photodimer into a B-DNA helix.

5. Base-base mismatches. Thermodynamics of double helix formation for dCA3XA3G + dCT3YT3G (X, Y = A,C,G,T).

6. Hairpin formation in synthetic oligonucleotides.

1. Escherichia coli DNA polymerase III can replicate efficiently past a T-T cis-syn cyclobutane dimer if DNA polymerase V and the 3' to 5' exonuclease proofreading function encoded by dnaQ are inactivated.

2. Crystal structure of a DNA decamer containing a cis-syn thymine dimer.

3. Replication past O(6)-methylguanine by yeast and human DNA polymerase eta.

4. T-T cyclobutane dimers are misinstructive, rather than non-instructive, mutagenic lesions.

5. Mechanism of nucleotide incorporation opposite a thymine-thymine dimer by yeast DNA polymerase eta.

6. Requirement of Watson-Crick hydrogen bonding for DNA synthesis by yeast DNA polymerase eta.

7. Distinct mechanisms of cis-syn thymine dimer bypass by Dpo4 and DNA polymerase eta.

8. Base pair opening in a deoxynucleotide duplex containing a cis-syn thymine cyclobutane dimer lesion.

9. Thymine photodimer formation in DNA hairpins. Unusual conformations favor (6 - 4) vs. (2 + 2) adducts.

10. Error-free replicative bypass of (6-4) photoproducts by DNA polymerase zeta in mouse and human cells.