Lin-Yen Kuan1,2, Wei-Lung Chen1,2, Jiann-Hwa Chen1,2, Fei-Ting Hsu3, Tsu-Te Liu4, Wei-Ting Chen5, Kai-Lee Wang6, Wen-Chang Chen7,8, Yu-Chang Liu9,10,11, Wei-Shu Wang12,13. 1. Department of Emergency Medicine, Cathay General Hospital, Taipei, Taiwan, R.O.C. 2. School of Medicine, Fu Jen Catholic University, Taipei, Taiwan, R.O.C. 3. Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C. 4. Division of Gastroenterology, Department of Internal Medicine, National Yang-Ming University Hospital, Yilan, Taiwan, R.O.C. 5. Department of Psychiatry, Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan, R.O.C. 6. Department of Nursing, Ching Kuo Institute of Management and Health, Keelung, Taiwan, R.O.C. 7. Department of Diagnostic Radiology, Chang Gung Memorial Hospital, Chiayi Branch, Chang Gung University of Science and Technology, Chiayi, Taiwan, R.O.C. 8. Department of Medical Imaging and Radiological Science, Central Taiwan University of Science and Technology, Taichung, Taiwan, R.O.C. 9. Department of Medical Imaging and Radiological Science, Central Taiwan University of Science and Technology, Taichung, Taiwan, R.O.C. kevinyc.liu@gmail.com 11313@ymuh.ym.edu.tw. 10. Department of Radiation Oncology, National Yang-Ming University Hospital, Yilan, Taiwan, R.O.C. 11. Department of Radiation Oncology, Chang Bing Show-Chwan Memorial Hospital, Changhua, Taiwan, R.O.C. 12. Department of Medicine, National Yang-Ming University Hospital, Yilan, Taiwan, R.O.C. kevinyc.liu@gmail.com 11313@ymuh.ym.edu.tw. 13. School of Medicine, National Yang-Ming University, Taipei, Taiwan, R.O.C.
Abstract
BACKGROUND/AIM: The aim of the present study was to evaluate the anti-cancer effect of magnolol in hepatocellular carcinoma (HCC) cells in vitro. MATERIALS AND METHODS: HCC SK-Hep1 cells were treated with different concentrations of magnolol or PD98059 [extracellular-signal-regulated kinase (ERK) inhibitor] for 48 h, and then cell viability, apoptosis, signal transduction, expression of anti-apoptotic and metastasis-related proteins, and cell invasion were investigated by [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) assay, flow cytometry, nuclear factor kappa B (NF-ĸB) reporter gene, western blotting, and cell invasion assays. RESULTS: Magnolol significantly induced accumulation of sub-G1 phase and caspase-3 activation and inhibited NF-ĸB activation, cell invasion, expression of phosphorylated ERK (pERK), anti-apoptotic and metastatic-related proteins. ERK inactivation was required for magnolol-induced inhibition of metastatic potential of SK-Hep1 cells. CONCLUSION: Taken together, these results indicated that magnolol not only induced apoptosis, but also inhibited ERK-modulated metastatic potential of HCC SK-Hep1 cells. Copyright
BACKGROUND/AIM: The aim of the present study was to evaluate the anti-cancer effect of magnolol in hepatocellular carcinoma (HCC) cells in vitro. MATERIALS AND METHODS: HCC SK-Hep1 cells were treated with different concentrations of magnolol or PD98059 [extracellular-signal-regulated kinase (ERK) inhibitor] for 48 h, and then cell viability, apoptosis, signal transduction, expression of anti-apoptotic and metastasis-related proteins, and cell invasion were investigated by [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) assay, flow cytometry, nuclear factor kappa B (NF-ĸB) reporter gene, western blotting, and cell invasion assays. RESULTS:Magnolol significantly induced accumulation of sub-G1 phase and caspase-3 activation and inhibited NF-ĸB activation, cell invasion, expression of phosphorylated ERK (pERK), anti-apoptotic and metastatic-related proteins. ERK inactivation was required for magnolol-induced inhibition of metastatic potential of SK-Hep1 cells. CONCLUSION: Taken together, these results indicated that magnolol not only induced apoptosis, but also inhibited ERK-modulated metastatic potential of HCC SK-Hep1 cells. Copyright
Authors: Shuo Chen; Jiaqi Shen; Jing Zhao; Jiazhong Wang; Tao Shan; Junhui Li; Meng Xu; Xi Chen; Yang Liu; Gang Cao Journal: Front Oncol Date: 2020-12-02 Impact factor: 6.244