Yu-Shan Chen1, Rou Sun2, Wei-Lung Chen3,4, Yu-Chen Yau5, Chia-Ling Hsieh6,7, Ying-Ming Chiu8,9, Jiann-Hwa Chen10,11, Fei-Ting Hsu12, Jing-Gung Chung12, Chia-Jung Tsai13. 1. Department of Radiation Oncology, Show Chwan Memorial Hospital, Changhua, Taiwan, R.O.C. 2. Department of Medical Imaging, Taipei Medical University Hospital, Taipei, Taiwan, R.O.C. 3. Department of Emergency Medicine, Cathay General Hospital, Taipei, Taiwan, R.O.C. 4. School of Medicine, Fu Jen Catholic University, Taipei, Taiwan, R.O.C. 5. Department of Radiation Oncology, Chang Bing Show Chwan Memorial Hospital, Lukang, Taiwan, R.O.C. 6. The Ph.D. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan, R.O.C. chsieh2@tmu.edu.tw ymcgreen@yahoo.com.tw D7028@mail.cmuh.org.tw chenjiannhwa@yahoo.com.tw cgh08335@cgh.org.tw. 7. TMU Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei, Taiwan, R.O.C. 8. Rheumatology and Immunology Center, China Medical University Hospital, Taichung, Taiwan, R.O.C. chsieh2@tmu.edu.tw ymcgreen@yahoo.com.tw D7028@mail.cmuh.org.tw chenjiannhwa@yahoo.com.tw cgh08335@cgh.org.tw. 9. School of Medicine, China Medical University, Taichung, Taiwan, R.O.C. 10. Department of Emergency Medicine, Cathay General Hospital, Taipei, Taiwan, R.O.C. chsieh2@tmu.edu.tw ymcgreen@yahoo.com.tw D7028@mail.cmuh.org.tw chenjiannhwa@yahoo.com.tw cgh08335@cgh.org.tw. 11. School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan, R.O.C. 12. Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C. 13. Department of Medical Imaging and Radiological Sciences, I-Shou University, Kaohsiung, Taiwan, R.O.C.
Abstract
BACKGROUND/AIM: Radiation (RT) induced ERK/NF-κB in hepatocellular carcinoma (HCC) has been reported in our previous works; it weakens the toxicity of RT or triggers a radioresistance effect. Thus, combining RT with a suitable NF-κB inhibitor may sensitize HCC to RT. Magnolol, a bioactive compound, was known to have anti-inflammatory and anti-tumor functions. Here, we aimed to investigate whether magnolol may enhance anti-HCC efficacy of RT in vivo. MATERIALS AND METHODS: We established a Hep3B bearing mouse to evaluate the efficacy of the combination treatment of magnolol and RT. RESULTS: Most significantly, tumor volume and tumor weight inhibition was found in the combination group. Tumor immunohistochemistry staining also illustrated the suppression of RT-induced ERK/NF-κB-related proteins expression by magnolol. In addition, intrinsic apoptosis-related proteins, such as caspase-3 and -9, were markedly increased in the combination group. CONCLUSION: Magnolol may effectively enhance anti-HCC ability of RT by downregulating the expression of ERK/NF-κB-related proteins and increasing the expression of apoptosis-related proteins. Copyright
BACKGROUND/AIM: Radiation (RT) induced ERK/NF-κB in hepatocellular carcinoma (HCC) has been reported in our previous works; it weakens the toxicity of RT or triggers a radioresistance effect. Thus, combining RT with a suitable NF-κB inhibitor may sensitize HCC to RT. Magnolol, a bioactive compound, was known to have anti-inflammatory and anti-tumor functions. Here, we aimed to investigate whether magnolol may enhance anti-HCC efficacy of RT in vivo. MATERIALS AND METHODS: We established a Hep3B bearing mouse to evaluate the efficacy of the combination treatment of magnolol and RT. RESULTS: Most significantly, tumor volume and tumor weight inhibition was found in the combination group. Tumor immunohistochemistry staining also illustrated the suppression of RT-induced ERK/NF-κB-related proteins expression by magnolol. In addition, intrinsic apoptosis-related proteins, such as caspase-3 and -9, were markedly increased in the combination group. CONCLUSION:Magnolol may effectively enhance anti-HCC ability of RT by downregulating the expression of ERK/NF-κB-related proteins and increasing the expression of apoptosis-related proteins. Copyright
Authors: Nicolas Magné; Robert-Alain Toillon; Virginie Bottero; Céline Didelot; Paul Van Houtte; Jean-Pierre Gérard; Jean-François Peyron Journal: Cancer Lett Date: 2006-01-18 Impact factor: 8.679