Ahmad Tavakoli1, Angila Ataei-Pirkooh1, Gity Mm Sadeghi2, Farah Bokharaei-Salim1, Peyman Sahrapour3, Seyed J Kiani1, Mohsen Moghoofei4, Mohammad Farahmand5, Davod Javanmard1, Seyed H Monavari1. 1. Department of Medical Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran 449614535, Iran. 2. Department of Polymer Engineering & Color Technology, Amirkabir University of Technology, Tehran 1591634311, Iran. 3. Department of Medicine, Faculty of Medicine, Iran University of Medical Sciences, Tehran 1449614535, Iran. 4. Department of Microbiology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah 6716777816, Iran. 5. Department of Medical Virology, School of Public Health, Tehran University of Medical Sciences, Tehran 1417613151, Iran.
Abstract
AIM: We aimed to determine the possible inhibitory effects of zinc oxide nanoparticles (ZnO-NPs) and polyethylene glycol (PEG)-coated ZnO-NPs (ZnO-PEG-NPs) on herpes simplex virus type 1 (HSV-1). MATERIALS & METHODS: PEGylated ZnO-NPs were synthesized by the mechanical method. Antiviral activity was assessed by 50% tissue culture infectious dose (TCID50) and real-time PCR assays. To confirm the antiviral activity of ZnO-NPs on expression of HSV-1 antigens, indirect immunofluorescence assay was also conducted. RESULTS: 200 μg/ml ZnO-PEG-NPs could result in 2.5 log10 TCID50 reduction in virus titer, with inhibition rate of approximately 92% in copy number of HSV-1 genomic DNA. CONCLUSION: ZnO-PEG-NPs could be proposed as a new agent for efficient HSV-1 inhibition. Our results indicated that PEGylation is effective in reducing cytotoxicity and increasing antiviral activity of nanoparticles.
AIM: We aimed to determine the possible inhibitory effects of zinc oxide nanoparticles (ZnO-NPs) and polyethylene glycol (PEG)-coated ZnO-NPs (ZnO-PEG-NPs) on herpes simplex virus type 1 (HSV-1). MATERIALS & METHODS: PEGylated ZnO-NPs were synthesized by the mechanical method. Antiviral activity was assessed by 50% tissue culture infectious dose (TCID50) and real-time PCR assays. To confirm the antiviral activity of ZnO-NPs on expression of HSV-1 antigens, indirect immunofluorescence assay was also conducted. RESULTS: 200 μg/ml ZnO-PEG-NPs could result in 2.5 log10 TCID50 reduction in virus titer, with inhibition rate of approximately 92% in copy number of HSV-1 genomic DNA. CONCLUSION:ZnO-PEG-NPs could be proposed as a new agent for efficient HSV-1 inhibition. Our results indicated that PEGylation is effective in reducing cytotoxicity and increasing antiviral activity of nanoparticles.
Authors: Mina Michael Melk; Seham S El-Hawary; Farouk Rasmy Melek; Dalia Osama Saleh; Omar M Ali; Mohamed A El Raey; Nabil Mohamed Selim Journal: Int J Nanomedicine Date: 2021-12-18