Literature DB >> 3034585

Expression and rescuing of a cloned human tumour necrosis factor gene using an EBV-based shuttle cosmid vector.

D Kioussis, F Wilson, C Daniels, C Leveton, J Taverne, J H Playfair.   

Abstract

A cosmid vector carrying the Epstein-Barr virus origin of replication, the EBNA-1 gene, the hygromycin phosphotransferase (hph) gene and pBR322 sequences has been constructed. This cosmid can replicate autonomously in the nucleus of human tissue culture cells, even when it carries a 35-kb long insert. The cosmid can be rescued from the transfected cells by cloning it directly into ampicillin-sensitive Escherichia coli. A gene for human tumour necrosis factor (TNF) cloned into this cosmid vector was introduced in tissue culture cells, where it was transcribed into mature mRNA.

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Year:  1987        PMID： 3034585      PMCID： PMC553403          DOI： 10.1002/j.1460-2075.1987.tb04762.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  30 in total

1.  Effect of tumour necrosis factor on cultured human melanoma cells.

Authors:  L Helson; S Green; E Carswell; L J Old
Journal:  Nature       Date:  1975-12-25       Impact factor: 49.962

2.  Transformation of mammalian cells with genes from procaryotes and eucaryotes.

Authors:  M Wigler; R Sweet; G K Sim; B Wold; A Pellicer; E Lacy; T Maniatis; S Silverstein; R Axel
Journal:  Cell       Date:  1979-04       Impact factor: 41.582

3.  Expression of an antigen associated with acute lymphoblastic leukemia in human leukemia-lymphoma cell lines.

Authors:  J Minowada; G Janossy; M F Greaves; T Tsubota; B I Srivastava; S Morikawa; E Tatsumi
Journal:  J Natl Cancer Inst       Date:  1978-06       Impact factor: 13.506

4.  Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

Authors:  A J Berk; P A Sharp
Journal:  Cell       Date:  1977-11       Impact factor: 41.582

5.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

6.  A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels.

Authors:  L Wieslander
Journal:  Anal Biochem       Date:  1979-10-01       Impact factor: 3.365

7.  Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.

Authors:  R F Weaver; C Weissmann
Journal:  Nucleic Acids Res       Date:  1979-11-10       Impact factor: 16.971

8.  A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors:  F L Graham; A J van der Eb
Journal:  Virology       Date:  1973-04       Impact factor: 3.616

9.  Possible importance of macrophage-derived mediators in acute malaria.

Authors:  I A Clark; J L Virelizier; E A Carswell; P R Wood
Journal:  Infect Immun       Date:  1981-06       Impact factor: 3.441

10.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

Authors:  J M Chirgwin; A E Przybyla; R J MacDonald; W J Rutter
Journal:  Biochemistry       Date:  1979-11-27       Impact factor: 3.162
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  23 in total

1.  Shuttle vectors conferring hygromycin B resistance to E. coli and to mammalian cells. Differential expression of carboxyterminal fusion proteins.

Authors:  F A Asselbergs; R Pronk
Journal:  Mol Biol Rep       Date:  1992-11       Impact factor: 2.316

2.  Analysis of mammalian MUC1 genes reveals potential functionally important domains.

Authors:  A P Spicer; T Duhig; B S Chilton; S J Gendler
Journal:  Mamm Genome       Date:  1995-12       Impact factor: 2.957

3.  Structure and methylation of the human calcitonin/alpha-CGRP gene.

Authors:  P M Broad; A J Symes; R V Thakker; R K Craig
Journal:  Nucleic Acids Res       Date:  1989-09-12       Impact factor: 16.971

4.  Localization of the genes for tumor necrosis factor and lymphotoxin between the HLA class I and III regions by field inversion gel electrophoresis.

Authors:  J Ragoussis; K Bloemer; E H Weiss; A Ziegler
Journal:  Immunogenetics       Date:  1988       Impact factor: 2.846

5.  The structure of the human glutathione S-transferase pi gene.

Authors:  I G Cowell; K H Dixon; S E Pemble; B Ketterer; J B Taylor
Journal:  Biochem J       Date:  1988-10-01       Impact factor: 3.857

6.  Epstein-Barr virus replication studies and their application to vector design.

Authors:  P M Brickell; M S Patel
Journal:  Mol Biotechnol       Date:  1995-06       Impact factor: 2.695

7.  Structure of human glutathione S-transferase class Mu genes.

Authors:  J B Taylor; J Oliver; R Sherrington; S E Pemble
Journal:  Biochem J       Date:  1991-03-01       Impact factor: 3.857

8.  Epstein-Barr virus episome-based promoter function in human myeloid cells.

Authors:  C A Hauer; R R Getty; M L Tykocinski
Journal:  Nucleic Acids Res       Date:  1989-03-11       Impact factor: 16.971

9.  Efficient cDNA cloning by direct phenotypic correction of a mutant human cell line (HPRT-) using an Epstein-Barr virus-derived cDNA expression vector.

Authors:  P B Belt; W Jongmans; J de Wit; J H Hoeijmakers; P van de Putte; C Backendorf
Journal:  Nucleic Acids Res       Date:  1991-09-25       Impact factor: 16.971

10.  Complementation of a Chlamydomonas reinhardtii mutant using a genomic cosmid library.

Authors:  S Purton; J D Rochaix
Journal:  Plant Mol Biol       Date:  1994-02       Impact factor: 4.076
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