Modularization of three-dimensional gold nanoparticles/ferrocene/liposome cluster for electrochemical biosensor.

For electrochemical biosensors, just like a computer, the modularization and coordinated operation of different components will facilitate the development of versatile biosensors and effectively reduce costs. However, the efficient synergy between different modules is always difficult. It would be a beneficial way to construct the multi-functional module. In this work, a three-dimensional gold nanoparticles/ferrocene/liposome cluster (GFLC) is fabricated and explored as a building block for the fabrication of an electrochemical biosensor, in which gold nanoparticles, ferrocene and liposome cluster work as a signal amplification component, a signal output component and a molecular recognition component, respectively. With the synergy of multi-functions, GFLC has been successfully applied for electrochemical analysis of lipopolysaccharide (LPS) in food samples. LPS can be linearly assayed in the range from 2 × 10-9 μg/mL to 8 μg/mL with a detection limit of 0.51 × 10-10 μg/mL. In view of the favorable modularization effect, GFLC shows a great potential in the development of electrochemical biosensor with considerable versatility and cost-efficiency.