Literature DB >> 3034186

Construction of a transposon containing a gene for polygalacturonate trans-eliminase from Klebsiella oxytoca.

M J Walker, J M Pemberton.   

Abstract

A DNA fragment containing a Klebsiella oxytoca gene for polygalacturonate trans-eliminase was cloned into the kanamycin resistance transposon Tn5. This new transposon, designated Tn5-Pga+, had a transposition frequency of 1 X 10(-6). The broad host range plasmid pR751::Tn5-Pga+ was conjugally transferred to a variety of genetic backgrounds. The ability to degrade polygalacturonate was expressed in Aeromonas hydrophila, Alcaligenes eutrophus, Azotomonas insolita, Escherichia coli, Pseudomonas putida and Rhodopseudomonas sphaeroides, but not in Zymomonas mobilis.

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Year:  1987        PMID： 3034186     DOI： 10.1007/BF00410941

Source DB:  PubMed          Journal:  Arch Microbiol        ISSN： 0302-8933            Impact factor:   2.552


  20 in total

1.  Construction of plasmid vectors with unique PstI cloning sites in a signal sequence coding region.

Authors:  K Talmadge; W Gilberg
Journal:  Gene       Date:  1980-12       Impact factor: 3.688

2.  A small cosmid for efficient cloning of large DNA fragments.

Authors:  B Hohn; J Collins
Journal:  Gene       Date:  1980-11       Impact factor: 3.688

3.  Novel bacteriophage lambda cloning vector.

Authors:  J Karn; S Brenner; L Barnett; G Cesareni
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

4.  The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

Authors:  J Vieira; J Messing
Journal:  Gene       Date:  1982-10       Impact factor: 3.688

5.  Multimerization of high copy number plasmids causes instability: CoIE1 encodes a determinant essential for plasmid monomerization and stability.

Authors:  D K Summers; D J Sherratt
Journal:  Cell       Date:  1984-04       Impact factor: 41.582

6.  Plasmids pEMBLY: new single-stranded shuttle vectors for the recovery and analysis of yeast DNA sequences.

Authors:  C Baldari; G Cesareni
Journal:  Gene       Date:  1985       Impact factor: 3.688

7.  Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors:  S N Cohen; A C Chang; L Hsu
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

8.  Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas.

Authors:  M Bagdasarian; R Lurz; B Rückert; F C Franklin; M M Bagdasarian; J Frey; K N Timmis
Journal:  Gene       Date:  1981-12       Impact factor: 3.688

9.  Enzymatic degradation of polygalacturonic acid by Yersinia and Klebsiella species in relation to clinical laboratory procedures.

Authors:  M P Starr; A K Chatterjee; P B Starr; G E Buchanan
Journal:  J Clin Microbiol       Date:  1977-10       Impact factor: 5.948

10.  Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325.

Authors:  X Soberon; L Covarrubias; F Bolivar
Journal:  Gene       Date:  1980-05       Impact factor: 3.688
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  4 in total

1.  Construction of a Tn5 derivative encoding bioluminescence and its introduction in Pseudomonas, Agrobacterium and Rhizobium.

Authors:  R Boivin; F P Chalifour; P Dion
Journal:  Mol Gen Genet       Date:  1988-07

2.  Molecular cloning and sequencing of a pectate lyase gene from Yersinia pseudotuberculosis.

Authors:  S Manulis; D Y Kobayashi; N T Keen
Journal:  J Bacteriol       Date:  1988-04       Impact factor: 3.490

3.  IS26-Mediated Formation of Transposons Carrying Antibiotic Resistance Genes.

Authors:  Christopher J Harmer; Ruth M Hall
Journal:  mSphere       Date:  2016-04-06       Impact factor: 4.389

4.  Sequences of two related multiple antibiotic resistance virulence plasmids sharing a unique IS26-related molecular signature isolated from different Escherichia coli pathotypes from different hosts.

Authors:  Carola Venturini; Karl A Hassan; Piklu Roy Chowdhury; Ian T Paulsen; Mark J Walker; Steven P Djordjevic
Journal:  PLoS One       Date:  2013-11-04       Impact factor: 3.240
  4 in total

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