Literature DB >> 3896935

Plasmids pEMBLY: new single-stranded shuttle vectors for the recovery and analysis of yeast DNA sequences.

C Baldari, G Cesareni.   

Abstract

We describe the construction and properties of pEMBLY plasmids. They belong to a new family of yeast shuttle vectors which are derived from plasmid vector pEMBL9 and offer the following improvement: relatively small size; large number of cloning sites; screening for insert-containing plasmids on indicator plates; different combinations of genes which complement auxotrophic deficiencies and sequences that support DNA replication in Saccharomyces cerevisiae; and ability to isolate the plasmid DNA in single-stranded (ss) form. The yeast S. cerevisiae can be efficiently transformed by these plasmids in both the ss and double-stranded (ds) forms. Finally, the presence of the phage f1 intergenic region allows one to obtain the cloned sequences in the ss form upon infection with the wild-type ss phage [Dotto et al., Virology 114 (1981) 463-473].

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Year:  1985        PMID： 3896935     DOI： 10.1016/0378-1119(85)90154-4

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  71 in total

1.  Physical and genetic mapping of Candida albicans: several genes previously assigned to chromosome 1 map to chromosome R, the rDNA-containing linkage group.

Authors:  B Wickes; J Staudinger; B B Magee; K J Kwon-Chung; P T Magee; S Scherer
Journal:  Infect Immun       Date:  1991-07       Impact factor: 3.441

2.  Isolation of a metal-activated transcription factor gene from Candida glabrata by complementation in Saccharomyces cerevisiae.

Authors:  P B Zhou; D J Thiele
Journal:  Proc Natl Acad Sci U S A       Date:  1991-07-15       Impact factor: 11.205

3.  Novel class of nuclear genes involved in both mRNA splicing and protein synthesis in Saccharomyces cerevisiae mitochondria.

Authors:  E B Asher; O Groudinsky; G Dujardin; N Altamura; M Kermorgant; P P Slonimski
Journal:  Mol Gen Genet       Date:  1989-02

4.  Different classes of polyadenylation sites in the yeast Saccharomyces cerevisiae.

Authors:  S Irniger; C M Egli; G H Braus
Journal:  Mol Cell Biol       Date:  1991-06       Impact factor: 4.272

5.  Cytochrome c impaled: investigation of the extended lipid anchorage of a soluble protein to mitochondrial membrane models.

Authors:  Erta Kalanxhi; Carmichael J A Wallace
Journal:  Biochem J       Date:  2007-10-15       Impact factor: 3.857

6.  The yeast DNA ligase gene CDC9 is controlled by six orientation specific upstream activating sequences that respond to cellular proliferation but which alone cannot mediate cell cycle regulation.

Authors:  J H White; A L Johnson; N F Lowndes; L H Johnston
Journal:  Nucleic Acids Res       Date:  1991-01-25       Impact factor: 16.971

7.  Genome-wide transposon mutagenesis in Saccharomyces cerevisiae and Candida albicans.

Authors:  Tao Xu; Nikë Bharucha; Anuj Kumar
Journal:  Methods Mol Biol       Date:  2011

8.  A new family of polymorphic genes in Saccharomyces cerevisiae: alpha-galactosidase genes MEL1-MEL7.

Authors:  G Naumov; H Turakainen; E Naumova; S Aho; M Korhola
Journal:  Mol Gen Genet       Date:  1990-10

9.  MET4, a leucine zipper protein, and centromere-binding factor 1 are both required for transcriptional activation of sulfur metabolism in Saccharomyces cerevisiae.

Authors:  D Thomas; I Jacquemin; Y Surdin-Kerjan
Journal:  Mol Cell Biol       Date:  1992-04       Impact factor: 4.272

10.  Efficient UV stimulation of yeast integrative transformation requires damage on both plasmid strands.

Authors:  M Ninković; M Alacević; F Fabre; Z Zgaga
Journal:  Mol Gen Genet       Date:  1994-05-10
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