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Literature DB >> 3034185

Methylation sensitivity of the restriction enzymes FnuDII and AccII.

Abstract

The restriction enzymes FnuDII and AccII are isoschizomers of the DNA sequence 5'-CGCG-3'. We have determined that 5-methylcytidine at either cytidine position in this recognition sequence inhibits DNA cleavage by FnuDII and AccII. A third isoschizomer, ThaI was previously shown to exhibit an identical methylation sensitivity. It is remarkable that 3 restriction enzymes derived from diverse microbiological sources exhibit this identical methylation sensitivity.

Entities: Chemical

Mesh：

Substances：

Year: 1987 PMID： 3034185 DOI： 10.1007/BF00410932

Source DB: PubMed Journal: Arch Microbiol ISSN： 0302-8933 Impact factor: 2.552

8 in total

1. The effect of site specific methylation on restriction endonuclease digestion.

Authors: M McClelland; M Nelson
Journal: Nucleic Acids Res Date: 1985 Impact factor: 16.971

2. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.

Authors: J G Sutcliffe
Journal: Cold Spring Harb Symp Quant Biol Date: 1979

3. Nucleotide sequence specificity of restriction endonucleases.

Authors: H O Smith
Journal: Science Date: 1979-08-03 Impact factor: 47.728

4. Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF.

Authors: S Jentsch
Journal: J Bacteriol Date: 1983-11 Impact factor: 3.490

5. Restriction and modification enzymes and their recognition sequences.

Authors: R J Roberts
Journal: Nucleic Acids Res Date: 1984 Impact factor: 16.971

6. Site specific endonuclease from Fusobacterium nucleatum.

Authors: A C Lui; B C McBride; G F Vovis; M Smith
Journal: Nucleic Acids Res Date: 1979-01 Impact factor: 16.971

7. Site-specific deoxyribonucleases in Bacillus subtilis and other Bacillus strains.

Authors: T Shibata; S Ikawa; C Kim; T Ando
Journal: J Bacteriol Date: 1976-10 Impact factor: 3.490

8. Methylation of either cytosine in the recognition sequence CGCG inhibits ThaI cleavage of DNA.

Authors: J S Strobl; E B Thompson
Journal: Nucleic Acids Res Date: 1984-11-12 Impact factor: 16.971

8 in total

5 in total

Methylation sensitivity of the restriction enzymes FnuDII and AccII.

1. The effect of site specific methylation on restriction endonuclease digestion.

2. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.

3. Nucleotide sequence specificity of restriction endonucleases.

4. Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF.

5. Restriction and modification enzymes and their recognition sequences.

6. Site specific endonuclease from Fusobacterium nucleatum.

7. Site-specific deoxyribonucleases in Bacillus subtilis and other Bacillus strains.

8. Methylation of either cytosine in the recognition sequence CGCG inhibits ThaI cleavage of DNA.

1. Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

2. Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.

3. Effect of site-specific methylation on restriction endonucleases and DNA modification methyltransferases.

4. Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases.

5. Cloning and characterization of the TneDI restriction: modification system of Thermotoga neapolitana.