Yuan Chen1, Elizabeth M Topp2. 1. Department of Industrial and Physical Pharmacy, College of Pharmacy, Purdue University, West Lafayette, Indiana 47907. 2. Department of Industrial and Physical Pharmacy, College of Pharmacy, Purdue University, West Lafayette, Indiana 47907. Electronic address: topp@purdue.edu.
Abstract
In this mini-review, the major types of photolytic labeling reagents are presented together with their reaction mechanisms. The applications of photolytic labeling in protein drug discovery and development are then discussed; these have expanded from studies of protein-protein interactions in vivo to protein-matrix interactions in lyophilized solids. The mini-review concludes with recommendations for further development of the approach, which include the need for new and more chemically diverse photo-reactive reagents and better understanding of the mechanisms of photolytic labeling reactions in various media.
In this mini-review, the major types of photolytic labeling reagents are presented together with their ren class="Chemical">action mechanisms. The applications of photolytic labeling in protein drug discovery and development are then discussed; these have expanded from studies of protein-protein interactions in vivo to protein-matrix interactions in lyophilized solids. The mini-review concludes with recommendations for further development of the approach, which include the need for new and more chemically diverse photo-reactive reagents and better understanding of the mechanisms of photolytic labeling reactions in various media.
Authors: Wei-Qin Zhao; Francesca Santini; Robert Breese; Dave Ross; Xiaohua Douglas Zhang; David J Stone; Marc Ferrer; Matthew Townsend; Abigail L Wolfe; Matthew A Seager; Gene G Kinney; Paul J Shughrue; William J Ray Journal: J Biol Chem Date: 2009-12-23 Impact factor: 5.157