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Literature DB >> 3033643

Structure and expression of human dihydropteridine reductase.

J Lockyer, R G Cook, S Milstien, S Kaufman, S L Woo, F D Ledley.

Abstract

Dihydropteridine reductase (DHPR; EC 1.6.99.7) catalyzes the NADH-mediated reduction of quinonoid dihydrobiopterin and is an essential component of the pterin-dependent aromatic amino acid hydroxylating systems. A cDNA for human DHPR was isolated from a human liver cDNA library in the vector lambda gt11 using a monospecific antibody against sheep DHPR. The nucleic acid sequence and amino acid sequence of human DHPR were determined from a full-length clone. A 112 amino acid sequence of sheep DHPR was obtained by sequencing purified sheep DHPR. This sequence is highly homologous to the predicted amino acid sequence of the human protein. Gene transfer of the recombinant human DHPR into COS cells leads to expression of DHPR enzymatic activity. These results indicate that the cDNA clone identified by antibody screening is an authentic and full-length cDNA for human DHPR.

Entities: Chemical Gene Species

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Year: 1987 PMID： 3033643 PMCID： PMC304863 DOI： 10.1073/pnas.84.10.3329

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

36 in total

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13 in total

1. Identity of 4a-carbinolamine dehydratase, a component of the phenylalanine hydroxylation system, and DCoH, a transregulator of homeodomain proteins.

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Journal: Proc Natl Acad Sci U S A Date: 1992-12-15 Impact factor: 11.205

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Authors: A Romstad; H S Kalkanoğlu; T Coşkun; M Demirkol; A Tokatli; A Dursun; T Baykal; I Ozalp; P Guldberg; F Güttler
Journal: Hum Genet Date: 2000-12 Impact factor: 4.132

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Authors: De-Yun Lu; Jun Ye; Lian-Shu Han; Wen-Juan Qiu; Hui-Wen Zhang; Jian-De Zhou; Pei-Zhong Bao; Ya-Fen Zhang; Xue-Fan Gu
Journal: World J Pediatr Date: 2014-08-15 Impact factor: 2.764

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Journal: Am J Hum Genet Date: 1994-05 Impact factor: 11.025

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Authors: D Concolino; G Muzzi; M Rapsomaniki; M T Moricca; M G Pascale; P Strisciuglio
Journal: J Inherit Metab Dis Date: 2008-04-15 Impact factor: 4.982