| Literature DB >> 30333092 |
Takeshi Hatani1, Shunsuke Funakoshi2, Thomas J Deerinck3, Eric A Bushong3, Takeshi Kimura4, Hiroshi Takeshima5, Mark H Ellisman6, Masahiko Hoshijima7, Yoshinori Yoshida8.
Abstract
Many studies have shown the feasibility of in vivo cardiac transplantation of human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) in animal experiments. However, nano-structural confirmation of the successful incorporation of the engrafted iPSC-CMs including electron microscopy (EM) has not been accomplished, partly because identification of graft cells in EM has proven to be difficult. Using APEX2, an engineered ascorbate peroxidase imaging tag, we successfully localized and analyzed the fine structure of sarcomeres and the excitation contraction machinery of iPSC-CMs 6 months after their engraftment in infarcted mouse hearts. APEX2 made iPSC-CMs visible in multiple imaging modalities including light microscopy, X-ray microscopic tomography, transmission EM, and scanning EM. EM tomography allowed assessment of the differentiation state of APEX2-positive iPSC-CMs and analysis of the fine structure of the sarcomeres including T-tubules and dyads.Entities:
Keywords: APEX2; Cardiac cell transplantation; Electron microscopy; Regeneration therapy; iPSC-derived cardiomyocytes
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Year: 2018 PMID： 30333092 PMCID： PMC7478876 DOI： 10.1016/j.bbrc.2018.10.020
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575