Literature DB >> 3033307

A baculovirus vector can express intron-containing genes.

K T Jeang, M Holmgren-Konig, G Khoury.   

Abstract

A simjan virus 40 genomic fragment containing the genes coding for the large T and small t antigens was inserted into the genome of the baculovirus Autographa californica nuclear polyhedrosis virus downstream of the strong polyhedrin promoter. Infection of eucaryotic Spodoptera frugiperda (SF9) cells with this recombinant virus produced significant amounts of small t antigen and little or no large T protein. Analysis by Northern blotting and S1 nuclease digestion revealed correct and preferential utilization of the small t splicing signals.

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Year:  1987        PMID： 3033307      PMCID： PMC254173          DOI： 10.1128/JVI.61.5.1761-1764.1987

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  19 in total

1.  Mapping of Early and Late Transcripts Encoded by the Autographa californica Nuclear Polyhedrosis Virus Genome: Is Viral RNA Spliced?

Authors:  H Lübbert; W Doerfler
Journal:  J Virol       Date:  1984-05       Impact factor: 5.103

2.  Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

Authors:  A J Berk; P A Sharp
Journal:  Cell       Date:  1977-11       Impact factor: 41.582

3.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

4.  Construction of an adenovirus-SV40 recombinant producing SV40 T antigen from an adenovirus late promoter.

Authors:  D Solnick
Journal:  Cell       Date:  1981-04       Impact factor: 41.582

5.  Properties of simian virus 40 small t antigen overproduced in bacteria.

Authors:  C S Thummel; T L Burgess; R Tjian
Journal:  J Virol       Date:  1981-02       Impact factor: 5.103

6.  A cycloheximide-enhanced protein in cytomegalovirus-infected cells.

Authors:  K T Jeang; W Gibson
Journal:  Virology       Date:  1980-12       Impact factor: 3.616

7.  Purification of biologically active simian virus 40 small tumor antigen.

Authors:  I Bikel; T M Roberts; M T Bladon; R Green; E Amann; D M Livingston
Journal:  Proc Natl Acad Sci U S A       Date:  1983-02       Impact factor: 11.205

8.  Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

Authors:  H Aviv; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1972-06       Impact factor: 11.205

9.  Ovalbumin is synthesized in mouse cells transformed with the natural chicken ovalbumin gene.

Authors:  E C Lai; S L Woo; M E Bordelon-Riser; T H Fraser; B W O'Malley
Journal:  Proc Natl Acad Sci U S A       Date:  1980-01       Impact factor: 11.205

10.  Production of human beta interferon in insect cells infected with a baculovirus expression vector.

Authors:  G E Smith; M D Summers; M J Fraser
Journal:  Mol Cell Biol       Date:  1983-12       Impact factor: 4.272
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  9 in total

Review 1.  Expression of eukaryotic genes in insect cultures.

Authors:  M J Fraser
Journal:  In Vitro Cell Dev Biol       Date:  1989-03

2.  Synthesis of functional human immunodeficiency virus tat protein in baculovirus as determined by a cell-cell fusion assay.

Authors:  K T Jeang; P R Shank; A B Rabson; A Kumar
Journal:  J Virol       Date:  1988-10       Impact factor: 5.103

3.  Purification and functional properties of simian virus 40 large and small T antigens overproduced in insect cells.

Authors:  C I Murphy; B Weiner; I Bikel; H Piwnica-Worms; M K Bradley; D M Livingston
Journal:  J Virol       Date:  1988-08       Impact factor: 5.103

4.  Multiple early transcripts and splicing of the Autographa californica nuclear polyhedrosis virus IE-1 gene.

Authors:  G E Chisholm; D J Henner
Journal:  J Virol       Date:  1988-09       Impact factor: 5.103

5.  Tissue-specific expression of silkmoth chorion genes in vivo using Bombyx mori nuclear polyhedrosis virus as a transducing vector.

Authors:  K Iatrou; R G Meidinger
Journal:  Proc Natl Acad Sci U S A       Date:  1990-05       Impact factor: 11.205

6.  Recombinant baculoviruses as vectors for identifying proteins encoded by intron-containing members of complex multigene families.

Authors:  K Iatrou; R G Meidinger; M R Goldsmith
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

7.  Expression of Aleutian mink disease parvovirus proteins in a baculovirus vector system.

Authors:  J Christensen; T Storgaard; B Bloch; S Alexandersen; B Aasted
Journal:  J Virol       Date:  1993-01       Impact factor: 5.103

8.  Expression and complex formation of simian virus 40 large T antigen and mouse p53 in insect cells.

Authors:  D R O'Reilly; L K Miller
Journal:  J Virol       Date:  1988-09       Impact factor: 5.103

9.  Exploiting the Intron-splicing Mechanism of Insect Cells to Produce Viral Vectors Harboring Toxic Genes for Suicide Gene Therapy.

Authors:  Haifeng Chen
Journal:  Mol Ther Nucleic Acids       Date:  2012-11-27       Impact factor: 10.183
  9 in total

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