Literature DB >> 2457111

Expression and complex formation of simian virus 40 large T antigen and mouse p53 in insect cells.

D R O'Reilly1, L K Miller.   

Abstract

Recombinant baculoviruses were constructed which express simian virus 40 large T antigen (SVT-Ag) or murine p53 to high levels in infected insect cells. Characterization of the expressed proteins revealed that they display many properties of the corresponding mammalian-derived proteins. Both proteins are of wild-type size, localize to the nucleus, are recognized by several SVT-Ag- or p53-specific monoclonal antibodies, and are phosphorylated in this system. Complexes are formed between baculovirus-derived SVT-Ag and p53 after coinfection of insect cells with both recombinant viruses. After infection of insect cells with either virus individually, each protein can self-associate to form a variety of oligomeric species. Pulse-chase experiments indicated that both SVT-Ag and p53 are highly stable in insect cells, even in the absence of complex formation.

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Year:  1988        PMID： 2457111      PMCID： PMC253427     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  55 in total

1.  Co-operation between the p53 protein tumor antigen and platelet-poor plasma in the induction of cellular DNA synthesis.

Authors:  L Kaczmarek; M Oren; R Baserga
Journal:  Exp Cell Res       Date:  1986-01       Impact factor: 3.905

2.  Monoclonal antibodies specific for simian virus 40 tumor antigens.

Authors:  E Harlow; L V Crawford; D C Pim; N M Williamson
Journal:  J Virol       Date:  1981-09       Impact factor: 5.103

3.  Intracellular location and kinetics of complex formation between simian virus 40 T antigen and cellular protein p53.

Authors:  F I Schmieg; D T Simmons
Journal:  J Virol       Date:  1984-11       Impact factor: 5.103

4.  Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.

Authors:  E Y Chen; P H Seeburg
Journal:  DNA       Date:  1985-04

5.  Production of human alpha-interferon in silkworm using a baculovirus vector.

Authors:  S Maeda; T Kawai; M Obinata; H Fujiwara; T Horiuchi; Y Saeki; Y Sato; M Furusawa
Journal:  Nature       Date:  1985 Jun 13-19       Impact factor: 49.962

6.  The cellular oncogene p53 can be activated by mutagenesis.

Authors:  J R Jenkins; K Rudge; P Chumakov; G A Currie
Journal:  Nature       Date:  1985 Oct 31-Nov 6       Impact factor: 49.962

7.  Cellular immortalization by a cDNA clone encoding the transformation-associated phosphoprotein p53.

Authors:  J R Jenkins; K Rudge; G A Currie
Journal:  Nature       Date:  1984 Dec 13-19       Impact factor: 49.962

8.  Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector.

Authors:  G E Smith; G Ju; B L Ericson; J Moschera; H W Lahm; R Chizzonite; M D Summers
Journal:  Proc Natl Acad Sci U S A       Date:  1985-12       Impact factor: 11.205

9.  Cooperation between gene encoding p53 tumour antigen and ras in cellular transformation.

Authors:  L F Parada; H Land; R A Weinberg; D Wolf; V Rotter
Journal:  Nature       Date:  1984 Dec 13-19       Impact factor: 49.962

10.  Precise epitope mapping of the murine transformation-associated protein, p53.

Authors:  A Wade-Evans; J R Jenkins
Journal:  EMBO J       Date:  1985-03       Impact factor: 11.598
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  43 in total

1.  Characterization of the tumor suppressor protein p53 as a protein kinase C substrate and a S100b-binding protein.

Authors:  J Baudier; C Delphin; D Grunwald; S Khochbin; J J Lawrence
Journal:  Proc Natl Acad Sci U S A       Date:  1992-12-01       Impact factor: 11.205

2.  Interactions required for binding of simian virus 40 T antigen to the viral origin and molecular modeling of initial assembly events.

Authors:  Danielle K Reese; Kodangattil R Sreekumar; Peter A Bullock
Journal:  J Virol       Date:  2004-03       Impact factor: 5.103

3.  Regulation of expression of a baculovirus ecdysteroid UDPglucosyltransferase gene.

Authors:  D R O'Reilly; L K Miller
Journal:  J Virol       Date:  1990-03       Impact factor: 5.103

Review 4.  Expression of eukaryotic genes in insect cultures.

Authors:  M J Fraser
Journal:  In Vitro Cell Dev Biol       Date:  1989-03

5.  Transcription of the baculovirus polyhedrin gene reduces the levels of an antisense transcript initiated downstream.

Authors:  B G Ooi; L K Miller
Journal:  J Virol       Date:  1990-06       Impact factor: 5.103

6.  Production of selenomethionyl-derivatized proteins in baculovirus-infected insect cells.

Authors:  Ciarán N Cronin; Kheng B Lim; Joe Rogers
Journal:  Protein Sci       Date:  2007-07-27       Impact factor: 6.725

7.  Thermally inactivated simian virus 40 tsA58 mutant T antigen cannot initiate viral DNA replication in vitro.

Authors:  I Reynisdóttir; D R O'Reilly; L K Miller; C Prives
Journal:  J Virol       Date:  1990-12       Impact factor: 5.103

8.  Synthesis of DNA containing the simian virus 40 origin of replication by the combined action of DNA polymerases alpha and delta.

Authors:  S H Lee; T Eki; J Hurwitz
Journal:  Proc Natl Acad Sci U S A       Date:  1989-10       Impact factor: 11.205

9.  Differential induction of structural changes in the simian virus 40 origin of replication by T antigen.

Authors:  J A Borowiec; F B Dean; J Hurwitz
Journal:  J Virol       Date:  1991-03       Impact factor: 5.103

10.  Murine p53 inhibits the function but not the formation of SV40 T antigen hexamers and stimulates T antigen RNA helicase activity.

Authors:  P N Friedman; E H Wang; K Meerovitch; N Sonenberg; C Prives
Journal:  Chromosoma       Date:  1992       Impact factor: 4.316
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