Literature DB >> 3033300

Expression of polyomavirus large T antigen by using a baculovirus vector.

W C Rice, H E Lorimer, C Prives, L K Miller.   

Abstract

A gene encoding the large T antigen of polyomavirus was inserted into the baculovirus Autographa californica nuclear polyhedrosis virus so that gene expression was under the control of the strong, very late polyhedrin gene promoter. Significantly more large T antigen was produced in recombinant virus-infected insect cells than was observed in polyomavirus-transformed mouse cells. The insect-derived T antigen exhibited polyomavirus origin-specific DNA binding. The baculovirus expression system provides a convenient source of T antigen for in vitro studies.

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Year:  1987        PMID： 3033300      PMCID： PMC254162          DOI： 10.1128/JVI.61.5.1712-1716.1987

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  24 in total

1.  Polyomavirus small t antigen: overproduction in bacteria, purification, and utilization for monoclonal and polyclonal antibody production.

Authors:  D C Pallas; C Schley; M Mahoney; E Harlow; B S Schaffhausen; T M Roberts
Journal:  J Virol       Date:  1986-12       Impact factor: 5.103

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  DNA binding activity of polyoma virus large tumor antigen.

Authors:  S M Dilworth; A Cowie; R I Kamen; B E Griffin
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

4.  Expression of the large T protein of polyoma virus promotes the establishment in culture of "normal" rodent fibroblast cell lines.

Authors:  M Rassoulzadegan; Z Naghashfar; A Cowie; A Carr; M Grisoni; R Kamen; F Cuzin
Journal:  Proc Natl Acad Sci U S A       Date:  1983-07       Impact factor: 11.205

5.  Production of human alpha-interferon in silkworm using a baculovirus vector.

Authors:  S Maeda; T Kawai; M Obinata; H Fujiwara; T Horiuchi; Y Saeki; Y Sato; M Furusawa
Journal:  Nature       Date:  1985 Jun 13-19       Impact factor: 49.962

6.  Simian virus 40 and polyomavirus large tumor antigens have different requirements for high-affinity sequence-specific DNA binding.

Authors:  A Scheller; C Prives
Journal:  J Virol       Date:  1985-05       Impact factor: 5.103

7.  Species-specific in vitro synthesis of DNA containing the polyoma virus origin of replication.

Authors:  Y Murakami; T Eki; M Yamada; C Prives; J Hurwitz
Journal:  Proc Natl Acad Sci U S A       Date:  1986-09       Impact factor: 11.205

8.  Multiple binding sites for polyomavirus large T antigen within regulatory sequences of polyomavirus DNA.

Authors:  A Cowie; R Kamen
Journal:  J Virol       Date:  1984-12       Impact factor: 5.103

9.  Production of human beta interferon in insect cells infected with a baculovirus expression vector.

Authors:  G E Smith; M D Summers; M J Fraser
Journal:  Mol Cell Biol       Date:  1983-12       Impact factor: 4.272

10.  Expression of the influenza virus haemagglutinin in insect cells by a baculovirus vector.

Authors:  K Kuroda; C Hauser; R Rott; H D Klenk; W Doerfler
Journal:  EMBO J       Date:  1986-06       Impact factor: 11.598
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  12 in total

Review 1.  Expression of eukaryotic genes in insect cultures.

Authors:  M J Fraser
Journal:  In Vitro Cell Dev Biol       Date:  1989-03

2.  Loss of DNA-binding and new transcriptional trans-activation function in polyomavirus large T-antigen with mutation of zinc finger motif.

Authors:  A Bergqvist; M Nilsson; K Bondeson; G Magnusson
Journal:  Nucleic Acids Res       Date:  1990-05-11       Impact factor: 16.971

3.  Purification and functional properties of simian virus 40 large and small T antigens overproduced in insect cells.

Authors:  C I Murphy; B Weiner; I Bikel; H Piwnica-Worms; M K Bradley; D M Livingston
Journal:  J Virol       Date:  1988-08       Impact factor: 5.103

4.  Intracellular localization and DNA-binding properties of human papillomavirus type 18 E6 protein expressed with a baculovirus vector.

Authors:  S R Grossman; R Mora; L A Laimins
Journal:  J Virol       Date:  1989-01       Impact factor: 5.103

5.  Assembly of nascent DNA into nucleosome structures in simian virus 40 chromosomes by HeLa cell extract.

Authors:  K Sugasawa; Y Murakami; N Miyamoto; F Hanaoka; M Ui
Journal:  J Virol       Date:  1990-10       Impact factor: 5.103

6.  The DNA-binding properties of polyomavirus large T antigen are altered by ATP and other nucleotides.

Authors:  H E Lorimer; E H Wang; C Prives
Journal:  J Virol       Date:  1991-02       Impact factor: 5.103

7.  Species-specific replication of simian virus 40 DNA in vitro requires the p180 subunit of human DNA polymerase alpha-primase.

Authors:  F Stadlbauer; C Voitenleitner; A Brückner; E Fanning; H P Nasheuer
Journal:  Mol Cell Biol       Date:  1996-01       Impact factor: 4.272

8.  Polyomavirus large T antigen binds cooperatively to its multiple binding sites in the viral origin of DNA replication.

Authors:  Y C Peng; N H Acheson
Journal:  J Virol       Date:  1998-09       Impact factor: 5.103

9.  Expression and complex formation of simian virus 40 large T antigen and mouse p53 in insect cells.

Authors:  D R O'Reilly; L K Miller
Journal:  J Virol       Date:  1988-09       Impact factor: 5.103

10.  Simian virus 40 large T antigen stabilizes the TATA-binding protein-TFIIA complex on the TATA element.

Authors:  B Damania; P Lieberman; J C Alwine
Journal:  Mol Cell Biol       Date:  1998-07       Impact factor: 4.272
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