Pedro Carvalho Cassino1, Larissa Schimidt Rosseti2, Osmar Ignácio Ayala3, Marco Antônio Utrera Martines3, Luciane Candeloro Portugual4, Claudio Goncalves de Oliveira4, Iandara Schettert Silva5, Ruy de Araujo Caldas6. 1. Fellow PhD degree, Postgraduate Program in Health and Development, Universidade Federal do Mato Grosso do Sul (UFMS), Campo Grande-MS, Brazil. Conception and design of the study, acquisition and interpretation of data, manuscript preparation. 2. Fellow Master degree, Postgraduate Program in Health and Development, UFMS, Campo Grande-MS, Brazil. Veterinary assistance in the experimental study. 3. PhD, Associate Professor, Department of Biological and Health Sciences. UFMS, Campo Grande-MS, Brazil. Statistical analysis, histopathological examinations. 4. PhD, Associate Professor, Chemistry Institute. UFMS, Campo Grande-MS, Brazil. Technical procedures. 5. PhD, Associate Professor, Postgraduate Program in Health and Development in the Midwest Region, UFMS, Campo Grande-MS, Brazil. Conception and design of the study. 6. Senior Visiting Professor, Postgraduate Program in Health and Development in the Midwest Region, UFMS, Campo Grande-MS, Brazil. English language, manuscript writing.
Abstract
PURPOSE: To analyze the therapeutic potentials of different hydroxyapatites used for the correction of bone defects in rats. METHODS: Forty rats, male, albino wistar, were distributed in 4 groups. They were submitted to a 3.5 mm defect in tibia. They received low purity hydroxyapatite, Strontium hydroxyapatite and hydroxyapatite doped with gallium, having a seven day evaluation time. Histopathology slides were stained with hematoxylin-eosin, for morphological evaluation. Were analyzed inflammatory processes, necrosis, presence of osteoclasts and osteoblasts, presence of the material, presence of white cells, neovascularization and bone neoformation. RESULTS: It was observed that the groups HAPSr and HAPGa, presented better results of trabecular bone, hyaline cartilage and bone marrow more organized. CONCLUSION: There was improvement in the repair of the bone defect produced, showing that these hydroxyapatites are effective osteoinductive, osteoconductive, osteintegrant agents and have biocompatibility, and may be indicated for use in defect repairs.
PURPOSE: To analyze the therapeutic potentials of different hydroxyapatites used for the correction of bone defects in rats. METHODS: Forty rats, male, albino wistar, were distributed in 4 groups. They were submitted to a 3.5 mm defect in tibia. They received low purity hydroxyapatite, Strontium hydroxyapatite and hydroxyapatite doped with gallium, having a seven day evaluation time. Histopathology slides were stained with hematoxylin-eosin, for morphological evaluation. Were analyzed inflammatory processes, necrosis, presence of osteoclasts and osteoblasts, presence of the material, presence of white cells, neovascularization and bone neoformation. RESULTS: It was observed that the groups HAPSr and HAPGa, presented better results of trabecular bone, hyaline cartilage and bone marrow more organized. CONCLUSION: There was improvement in the repair of the bone defect produced, showing that these hydroxyapatites are effective osteoinductive, osteoconductive, osteintegrant agents and have biocompatibility, and may be indicated for use in defect repairs.
Authors: Julia Risso Parisi; Kelly Rossetti Fernandes; Matheus de Almeida Cruz; Ingrid Regina Avanzi; Alan de França Santana; Giovanna Caroline Aparecida do Vale; Ana Laura Martins de Andrade; Cíntia Pereira de Góes; Carlos Alberto Fortulan; Eliandra de Sousa Trichês; Renata Neves Granito; Ana Claudia Muniz Rennó Journal: Mar Biotechnol (NY) Date: 2020-04-25 Impact factor: 3.619
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