Barbara Moura Antunes1, Eduardo Zapaterra Campos1,2, Ronaldo Vagner Thomatieli Dos Santos3, José Cesar Rosa-Neto4, Emerson Franchini5, Nicolette C Bishop6, Fábio Santos Lira1. 1. Department of Physical Education, Exercise and Immunometabolism Research Group, Universidade Estadual Paulista, Presidente Prudente, São Paulo, Brazil. 2. Department of Physical Education, Federal University of Pernambuco, Recife, Brazil. 3. Department of Bioscience, Universidade Federal de São Paulo, Santos, São Paulo, Brazil. 4. Immunometabolism Research Group, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. 5. School of Physical Education and Sport, University of São Paulo, São Paulo, Brazil. 6. School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, United Kingdom.
Abstract
PURPOSE: The relationship between inflammatory markers and energetic metabolism has been explored. However, the relationship between exercise intensity and fitness status is unclear, and it is necessary to understand this relationship to apply specific exercise guidance. The purpose of the study was to analyze metabolic and inflammatory responses imposed by acute exercise sessions performed at moderate, heavy, and severe intensities and their relationship with the physical fitness status. METHODS: Nineteen healthy male volunteers performed three acute exercise sessions until exhaustion or up to 60 minutes on a cycle ergometer at moderate (90% of first ventilatory threshold [VT1]), heavy (midpoint between VT1 and second ventilatory threshold [VT2]), and severe (midpoint between VT2 and maximal aerobic power) intensities. Blood lactate, glucose, NEFA, endotoxin, and cytokines were determined for each exercise session. Peripheral and lipopolysaccharide (LPS)-stimulated release of tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, and IL-10 was analyzed before, after, and 60 minutes after sessions. RESULTS: In peripheral blood, severe intensity increased lactate, endotoxin, and TNF-α immediately after exercise and glucose at 60 min after exercise. There was a trend for IL-10 increase at 60 minutes after exercise in peripheral blood. Immediately after exercise, LPS-stimulated TNF-α, IL-6, IL-6/IL-10 ratio, and lactate levels were higher in the severe intensity while nonester fatty acid levels decreased at this time. At 60 minute after exercise, higher concentrations of glucose and a trend for increased IL-10 were observed in severe intensity. A positive correlation was observed between maximal aerobic power and IL-10 ( r = 0.513; P = 0.042), and negative correlations between maximal aerobic power and endotoxin ( r = -0.531; P = 0.034) and lactate ( r = -0.538; P = 0.031) in heavy intensity. CONCLUSION: Our data show a novel finding that higher cytokine responses occur at higher intensities, mainly in severe intensity. However, the anti-inflammatory (IL-10) response was physical fitness-dependent.
PURPOSE: The relationship between inflammatory markers and energetic metabolism has been explored. However, the relationship between exercise intensity and fitness status is unclear, and it is necessary to understand this relationship to apply specific exercise guidance. The purpose of the study was to analyze metabolic and inflammatory responses imposed by acute exercise sessions performed at moderate, heavy, and severe intensities and their relationship with the physical fitness status. METHODS: Nineteen healthy male volunteers performed three acute exercise sessions until exhaustion or up to 60 minutes on a cycle ergometer at moderate (90% of first ventilatory threshold [VT1]), heavy (midpoint between VT1 and second ventilatory threshold [VT2]), and severe (midpoint between VT2 and maximal aerobic power) intensities. Blood lactate, glucose, NEFA, endotoxin, and cytokines were determined for each exercise session. Peripheral and lipopolysaccharide (LPS)-stimulated release of tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, and IL-10 was analyzed before, after, and 60 minutes after sessions. RESULTS: In peripheral blood, severe intensity increased lactate, endotoxin, and TNF-α immediately after exercise and glucose at 60 min after exercise. There was a trend for IL-10 increase at 60 minutes after exercise in peripheral blood. Immediately after exercise, LPS-stimulated TNF-α, IL-6, IL-6/IL-10 ratio, and lactate levels were higher in the severe intensity while nonester fatty acid levels decreased at this time. At 60 minute after exercise, higher concentrations of glucose and a trend for increased IL-10 were observed in severe intensity. A positive correlation was observed between maximal aerobic power and IL-10 ( r = 0.513; P = 0.042), and negative correlations between maximal aerobic power and endotoxin ( r = -0.531; P = 0.034) and lactate ( r = -0.538; P = 0.031) in heavy intensity. CONCLUSION: Our data show a novel finding that higher cytokine responses occur at higher intensities, mainly in severe intensity. However, the anti-inflammatory (IL-10) response was physical fitness-dependent.
Authors: Eduardo da Silva Alves; Ronaldo Vagner Thomatiele Dos Santos; Fábio Santos de Lira; Alexandre Aparecido Almeida; Kate Edwards; Mateus Benvenutti; Sergio Tufik; Marco Túlio De Mello Journal: J Spinal Cord Med Date: 2020-04-16 Impact factor: 1.985
Authors: Ryan M Sapp; Catalina A Chesney; Lauren E Eagan; William S Evans; Evelyn M Zietowski; Steven J Prior; James M Hagberg; Sushant M Ranadive Journal: Physiol Rep Date: 2020-05
Authors: Gilson P Dorneles; Igor M da Silva; Maeli Andressa Santos; Viviane R Elsner; Simone G Fonseca; Alessandra Peres; Pedro R T Romão Journal: Sci Rep Date: 2020-12-10 Impact factor: 4.379
Authors: Henry B Ogden; Robert B Child; Joanne L Fallowfield; Simon K Delves; Caroline S Westwood; Joseph D Layden Journal: Nutrients Date: 2020-02-19 Impact factor: 5.717
Authors: Barbara Moura Antunes; José Cesar Rosa-Neto; Helena Angélica Pereira Batatinha; Emerson Franchini; Ana Maria Teixeira; Fábio Santos Lira Journal: Sci Rep Date: 2020-08-24 Impact factor: 4.379