Literature DB >> 3031608

Charons 36 to 40: multi enzyme, high capacity, recombination deficient replacement vectors with polylinkers and polystuffers.

I S Dunn, F R Blattner.   

Abstract

New phage lambda based cloning vectors, Charons 36-40, have been constructed which allow cloning of large (up to 24 kb) DNA fragments with up to sixteen cloning enzymes. Several of these could not be used previously with lambda vectors. Clones produced with these vectors can be propagated under recombination deficient conditions. A novel polystuffer method has been developed that permits vector arms to be purified by simple precipitation and which allows reliable identification of clones that have reincorporated any part of the stuffer. Three of the vectors are available with amber mutations in essential genes.

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Year:  1987        PMID： 3031608      PMCID： PMC340677          DOI： 10.1093/nar/15.6.2677

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  26 in total

1.  Insertion of DNA carrying ribosomal protein genes of Escherichia coli into Charon vector phages.

Authors:  B G Williams; F R Blattner; S R Jaskunas; M Nomura
Journal:  J Biol Chem       Date:  1977-10-25       Impact factor: 5.157

2.  EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system.

Authors:  P Leder; D Tiemeier; L Enquist
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

3.  Construction of coliphage lambda Charon vectors with BamHI cloning sites.

Authors:  D L Rimm; D Horness; J Kucera; F R Blattner
Journal:  Gene       Date:  1980-12       Impact factor: 3.688

4.  Novel bacteriophage lambda cloning vector.

Authors:  J Karn; S Brenner; L Barnett; G Cesareni
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

5.  New bacteriophage lambda vectors with positive selection for cloned inserts.

Authors:  J Karn; S Brenner; L Barnett
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

6.  Purification of genomic sequences from bacteriophage libraries by recombination and selection in vivo.

Authors:  B Seed
Journal:  Nucleic Acids Res       Date:  1983-04-25       Impact factor: 16.971

7.  Formal description of a DNA oriented computer language.

Authors:  J L Schroeder; F R Blattner
Journal:  Nucleic Acids Res       Date:  1982-01-11       Impact factor: 16.971

8.  Sequence of the lactose permease gene.

Authors:  D E Büchel; B Gronenborn; B Müller-Hill
Journal:  Nature       Date:  1980-02-07       Impact factor: 49.962

9.  Fractionation of DNA fragments by polyethylene glycol induced precipitation.

Authors:  J T Lis
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

10.  Purified lambda regulatory protein cII positively activates promoters for lysogenic development.

Authors:  H Simatake; M Rosenberg
Journal:  Nature       Date:  1981-07-09       Impact factor: 49.962
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  40 in total

1.  The nucleotide sequence of ribosomal 5S RNA from lettuce seeds.

Authors:  M Nalaskowska; T Opiola; J Barciszewski
Journal:  Nucleic Acids Res       Date:  1991-03-25       Impact factor: 16.971

2.  Precipitation of DNA by polyethylene glycol and ethanol.

Authors:  K R Paithankar; K S Prasad
Journal:  Nucleic Acids Res       Date:  1991-03-25       Impact factor: 16.971

3.  A 2.6 kb intron separates the signal peptide coding sequence of an anther-specific protein from the rest of the gene in sunflower.

Authors:  C Domon; J L Evrard; D T Pillay; A Steinmetz
Journal:  Mol Gen Genet       Date:  1991-10

4.  Immunoglobulin D switching can occur through homologous recombination in human B cells.

Authors:  M B White; C J Word; C G Humphries; F R Blattner; P W Tucker
Journal:  Mol Cell Biol       Date:  1990-07       Impact factor: 4.272

5.  Molecular characterization of a deletion-prone region of plasmid pAE1 of Alcaligenes eutrophus H1.

Authors:  W Y Chow; C K Wang; W L Lee; S S Kung; Y M Wu
Journal:  J Bacteriol       Date:  1995-07       Impact factor: 3.490

6.  The isolation and nucleotide sequence of the pyruvate decarboxylase gene from Kluyveromyces marxianus.

Authors:  P Holloway; R E Subden
Journal:  Curr Genet       Date:  1993-09       Impact factor: 3.886

7.  Genome sequencing on both strands: the Janus strategy.

Authors:  V Burland; D L Daniels; G Plunkett; F R Blattner
Journal:  Nucleic Acids Res       Date:  1993-07-25       Impact factor: 16.971

8.  Mycorrhizal morphotyping and molecular characterization of Chondrogaster angustisporus Giachini, Castellano, Trappe & Oliveira, an ectomycorrhizal fungus from Eucalyptus.

Authors:  M Lupatini; P A P Bonnassis; R B Steffen; V L Oliveira; Z I Antoniolli
Journal:  Mycorrhiza       Date:  2008-08-10       Impact factor: 3.387

9.  A 168 bp derivative of Suppressor-mutator/Enhancer is responsible for the maize o2-23 mutation.

Authors:  M J Aukerman; R J Schmidt
Journal:  Plant Mol Biol       Date:  1993-01       Impact factor: 4.076

10.  Maize regulatory gene opaque-2 encodes a protein with a "leucine-zipper" motif that binds to zein DNA.

Authors:  R J Schmidt; F A Burr; M J Aukerman; B Burr
Journal:  Proc Natl Acad Sci U S A       Date:  1990-01       Impact factor: 11.205
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